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Euphytica

, 214:2 | Cite as

Hybrids of Passiflora: P. gardneri versus P. gibertii, confirmation of paternity, morphological and cytogenetic characterization

  • Gabriela de Oliveira Belo
  • Margarete Magalhães Souza
  • Gonçalo Santos Silva
  • Manuella Palmeira Lavinscky
Article
  • 188 Downloads

Abstract

Two new varieties of interspecific hybrids of Passiflora have been developed from the cross between P. gardneri versus P. gibertii, both registered under the Passiflora Society International. Twelve putative hybrids were analyzed. Hybridization was confirmed using RAPD and SSR markers. Primer UBC11 (5′-CCGGCCTTAC-3′) generated informative bands. Primer SSR Pe75 has amplified species-specific fragments and a heterozygote status was observed with two parent bands 300 and 350 bp. The molecular markers generated have been analyzed for the presence or absence of specific informative bands. Based on the morphological characterization, we have identified two hybrid varieties: P. ‘Gabriela’ and P. ‘Bella’. P. ‘Gabriela’ produced flowers in bluish tones, bluish petals on the adaxial and abaxial faces, light blue sepals on the adaxial and light green on the abaxial faces, corona with the base of filaments in intense lilac color and white apex. P. ‘Bella’ produced flowers in lilac tones, intense lilac petals on the adaxial and abaxial faces, dark lilac sepals with whitish edges on the adaxial and light green on the abaxial faces, corona with the base of filaments in intense lilac color and white apex. The cytogenetic analysis verified that the hybrids have the same chromosomal number as the parents (2n = 18); the formation of bivalents between the homeologous chromosomes (n = 9) was observad, leading to regular meiosis, which allows the sexual reproduction and use of these hybrids in breeding programs.

Keywords

Interspecific hybrids Passion flower P. ‘Gabriela’ P. ‘Bella’ RAPD SSR 

Notes

Acknowledgements

Thanks to Dr. Armando Carlos Cervi (posthumous homage), a taxonomist specialist in Passiflora, for the identification of the genitors; UESC, CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and FAPESB (Fundação de Amparo à Pesquisa do Estado da Bahia) for their financial support to research; CAPES for the scholarships granted to the first and third authors; CNPq for the scholarship awarded to the second author.

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Laboratório de Melhoramento de Plantas, Departamento de Ciências BiológicasUniversidade Estadual de Santa Cruz (UESC)IlhéusBrazil

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