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Whole-genome re-sequencing and fine mapping of an orange petal color gene (Bnpc1) in spring Brassica napus L. to a 151-kb region

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Abstract

The petal color of B. napus is usually yellow, but white, cream, golden yellow, orange, and other colors have also been reported. The pigmentation mechanisms and evolutionary processes of petal color in B. napus remain elusive. An analysis of the inheritance of the orange petal color (OPC) trait indicated that this trait was controlled by two recessive genes, termed Bnpc1 and Bnpc2. For fine mapping of the Bnpc1 gene, two backcrossing populations, BC4 and BC5, were developed from the cross between an OPC parent, X118, and a yellow petal color parent, R258. Whole-genome re-sequencing with bulked segregation analysis using BC4 individuals was conducted, and as a result, two major candidate intervals of Bnpc1 were identified on chromosome C09 of B. napus (C09: 1.000–5.464 and 6.491–7.777 Mb). Subsequently, the genetic and physical linkage maps were constructed for the BC5 population using 10 simple sequence repeat, two sequence-characterized amplified region and 12 insertion-deletion (InDel) markers. The Bnpc1 gene was located in scaffold_38 on C09 of B. napus and flanked by Indel-31 and Indel-5, with genetic distances of 0.083 and 0.042 cM, respectively. The interval distance between the two markers was 151 kb (4.624–4.775 Mb) and was included in the first re-sequenced candidate region (C09: 1.000–5.464 Mb). The present study may facilitate cloning of the Bnpc1 gene as well as marker-assisted selection for developing OPC B. napus varieties and revealing the mechanism of petal color formation in B. napus.

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Abbreviations

OPC :

Orange petal color

YPC :

Yellow petal color

ZEP :

Zeaxanthin epoxidase

InDel:

Insertion-deletion

SSR:

Simple sequence repeat

SCAR:

Sequence-characterized amplified region

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Acknowledgements

The authors are grateful to Dr. Kun Lu and Dr. Zhi Zhao for critical technical assistance.

Funding

This research was financially supported by the National Key Research and Development Plan of China (2016YFD0100202), the Applied Basic Research Program of Qinghai Province (2015-ZJ-704), the National System of Technology of the Rapeseed Industry (NYCYTX-00516), and the Key Laboratory of Spring Rapeseed Genetic Improvement (2017-ZJ-Y09).

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  1. State Key Laboratory of Plateau Ecology and Agriculture of Qinghai University, Key Laboratory of Spring Rapeseed Genetic Improvement, Spring Rapeseed Research and Development Center of Qinghai Province, Academy of Agricultural and Forestry Sciences, Qinghai University, Xining, 810016, Qinghai, China

    Yanmei Yao, Kaixiang Li, Haidong Liu, Shaomin Guo, Lu Xiao & Dezhi Du

  2. Department of Plant Science, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada

    Robert W. Duncan

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  1. Yanmei Yao
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  2. Kaixiang Li
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Yao, Y., Li, K., Liu, H. et al. Whole-genome re-sequencing and fine mapping of an orange petal color gene (Bnpc1) in spring Brassica napus L. to a 151-kb region. Euphytica 213, 165 (2017). https://doi.org/10.1007/s10681-017-1959-4

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