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Mapping of new resistance (Vr2, Rm1) and ornamental (Di2, pl) Mendelian trait loci in peach

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Abstract

Peach powdery mildew is one of the major diseases of the peach. Various sources of resistance to PPM have thus been identified, including the single dominant locus Vr2 carried by the peach rootstock ‘Pamirskij 5’. To map Vr2, a linkage map based on microsatellite markers was constructed from the F2 progeny (WP2) derived from the cross ‘Weeping Flower Peach’ × ‘Pamirskij 5’. Self-pollinations of the parents were also performed. Under greenhouse conditions, all progenies were scored after artificial inoculations in two classes of reactions to PPM (resistant/susceptible). In addition to Vr2, WP2 segregated for three other traits from ‘Weeping Flower Peach’: Rm1 for green peach aphid resistance, Di2 for double-flower and pl for weeping-growth habit. With their genomic locations unknown or underdocumented, all were phenotyped as Mendelian characters and mapped: Vr2 mapped at the top of LG8, at 3.3 cM, close to the CPSCT018 marker; Rm1 mapped at the bottom of LG1, at a position of 116.5 cM, cosegregating with the UDAp-467 marker and in the same region as Rm2 from ‘Rubira’®; Di2 mapped at 28.8 cM on LG6, close to the MA027a marker; and pl mapped at 44.1 cM on LG3 between the MA039a and SSRLG3_16m46 markers. Furthermore, this study revealed, for the first time, a pseudo-linkage between two traits of the peach: Vr2 and the Gr locus, which controls the red/green color of foliage. The present work therefore constitutes a significant preliminary step for implementing marker-assisted selection for the four major traits targeted in this study.

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Acknowledgements

This work was funded by grants from the INRA through the ‘InnovaFruit’ project. The authors would like to thank Stéphane Decroocq (INRA UMR-BFP, Bordeaux) for supplying the SSRLG3_16m46 marker used to map the pl locus and the staff of the Experimental Facilities of ‘Les Pins de L’Amarine’ and ‘Saint Maurice’ (INRA - UGAFL) for their technical contribution to tree management.

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  1. GAFL, INRA, 84140, Montfavet, France

    Thierry Pascal, Carole Confolent, Elodie Lecerf, Christophe Tuéro & Patrick Lambert

  2. Japan Agro Services SA, 30320, Marguerittes, France

    Romain Aberlenc

  3. HM CLAUSE, 49800, La Bohalle, France

    Mathilde Hoerter

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  1. Thierry Pascal
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  2. Romain Aberlenc
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  3. Carole Confolent
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Correspondence to Thierry Pascal.

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Pascal, T., Aberlenc, R., Confolent, C. et al. Mapping of new resistance (Vr2, Rm1) and ornamental (Di2, pl) Mendelian trait loci in peach. Euphytica 213, 132 (2017). https://doi.org/10.1007/s10681-017-1921-5

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