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Linkage map construction and QTL identification of P-deficiency tolerance in Oryza rufipogon Griff. at early seedling stage

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Abstract

Low phosphorus availability is a major factor limiting rice productivity. In this study, a population of backcross recombinant inbred lines (BILs) derived from an inter-specific cross (Oryza sativa L. × O. rufipogon Griff.) was used for genetic linkage map construction and quantitative trait locus (QTL) mapping. The results showed that a linkage map consisting of 153 markers was constructed. Twenty-one out of 231 BILs were tolerant of low-phosphorus according to the index to P-deficiency tolerance. Twenty-three QTLs on chromosomes 1, 2, 3, 7, 8, 9 and 11 were detected, of which eight QTLs showed high (22.93–37.32%) contribution to phenotypic variation. In addition, most of QTLs in this study (18 out of 23 QTLs) were located and overlapped on the chromosome 1, 3 and 11, which individually explained 6.07–34.70% phenotypic variation, indicating that there might be multiple main effect QTLs related to P-deficiency tolerance in O. rufipogon, and these QTLs might cluster in the same region. These results would provide helpful information for cloning and utilizing the P-deficiency tolerance-responsive genes from O. rufipogon.

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Acknowledgements

This research was partially supported by the National Natural Science Foundation of China (31260255, 31360147 and 31660384), the Development Program for Young Scientists of Jiangxi Province, China (20112BCB23007) and the Scientific Planning Project of Jiangxi Provincial Education Department (GJJ12184 and KJLD12059).

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Authors and Affiliations

  1. College of Life Science, Jiangxi Normal University, Nanchang, 330022, China

    Xiang-Dong Luo, Jian Liu, Liang-Fang Dai, Fan-Tao Zhang & Jian-Kun Xie

  2. Rice Research Institute, Jiangxi Academy of Agricultural Science, Nanchang, 330200, China

    Yong Wan

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  1. Xiang-Dong Luo
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  2. Jian Liu
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  3. Liang-Fang Dai
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  4. Fan-Tao Zhang
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  5. Yong Wan
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  6. Jian-Kun Xie
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Correspondence to Xiang-Dong Luo or Jian-Kun Xie.

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Luo, XD., Liu, J., Dai, LF. et al. Linkage map construction and QTL identification of P-deficiency tolerance in Oryza rufipogon Griff. at early seedling stage. Euphytica 213, 96 (2017). https://doi.org/10.1007/s10681-017-1884-6

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  • DOI: https://doi.org/10.1007/s10681-017-1884-6

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