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Obtainment of an intergeneric hybrid between Forsythia and Abeliophyllum

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Abstract

Forsythia suspensa and F. ‘Courtaneur’ were used as female parents to cross with Abeliophyllum distichum in 2011 and an intergeneric hybrid of F. suspensa × A. distichum was obtained, though with very low seed set. The morphological characteristics, flower fragrance and volatile organic compounds of flowers were analysed. The intergeneric hybrid had intermediate morphological characteristics of both parents and flower fragrance and was confirmed as a true intergeneric hybrid by amplified fragment length polymorphism (AFLP) markers. Compared with its mother parent (F. suspensa), flowers of the intergeneric hybrid are pale yellow with delicate fragrance. Volatile organic compounds of flowers were retrieved by purge-and-trap techniques, and determined by gas chromatography and mass spectrometry (GC–MS). The main volatile organic components of F. suspensa were isoprenoids, while the main volatile organic components of A. distichum and the hybrid of F. suspensa × A. distichum were aliphatics. To determine the time and the site of intergeneric hybridizing barriers occured, the pollen tubes’ behavior after pollination was observed under fluorescence microscopy. It was found that significant pre-fertilization incompatibility existed in intergeneric crossing combinations [F. ‘Courtaneur’ (Pin) × A. distichum (Thrum) and F. suspensa (Pin) × A. distichum (Thrum)], and only a few pollen tubes of A. distichum penetrated into the ovaries of Forsythia. In our research, an intergeneric hybrid between Forsythia and Abeliophyllum was obtained for the first time, which will provide a solid foundation for expanding the flower color range of Forsythia and breeding fragrant-flowered cultivars.

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Acknowledgements

This study was funded by by the Fundamental Research Fund for the Central University (2015ZCQ-YL-03).

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  1. JianShuang Shen and TingLiang Xu have contributed equally to this work.

Authors and Affiliations

  1. Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment and College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, China

    JianShuang Shen, TingLiang Xu, Chao Shi, TangRen Cheng, Jia Wang, HuiTang Pan & QiXiang Zhang

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  1. JianShuang Shen
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  2. TingLiang Xu
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  3. Chao Shi
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Correspondence to HuiTang Pan.

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Shen, J., Xu, T., Shi, C. et al. Obtainment of an intergeneric hybrid between Forsythia and Abeliophyllum . Euphytica 213, 95 (2017). https://doi.org/10.1007/s10681-017-1880-x

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