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Development and characterization of an eggplant (Solanum melongena) doubled haploid population and a doubled haploid line with high androgenic response

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Abstract

We developed an eggplant doubled haploid (DH) population from a commercial hybrid through androgenesis in microspore culture. Morphological variation, reproductive ability and androgenic responsiveness were evaluated. The DH population showed segregation in vegetative traits related to leaf, flower and fruit, and in reproductive traits such as fruit and seed setting or germination rate. The DH population and subsequent generations also presented variation in the androgenic response, with null, low and high response lines. From this population, we were able to identify the first eggplant highly androgenic DH line (DH36), remarkably similar to the donor hybrid in terms of morphology and reproductive ability, but stably producing four times more calli than the hybrid. The segregating DH population is potentially useful for genetic studies and mapping of several traits, whereas the highly androgenic line DH36 may be used as a model line to facilitate the study of eggplant androgenesis and embryogenesis for both basic and applied research.

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References

  • Alpsoy HC, Seniz V (2007) Researches on the in vitro androgenesis and obtaining haploid plants in some eggplant genotypes. Acta Hortic 729:137–141

    Article  CAS  Google Scholar 

  • Başay S, Şeniz V, Ellialtioğlu Ş (2011) Obtaining dihaploid lines by using anther culture in the different eggplant cultivars. J Food Agric Environ 9:188–190

    Google Scholar 

  • Bohanec B (2002) Doubled-haploid onions. In: Rabinowitch HD, Currah L (eds) Allium crop science: recent advances. CABI Publishing, Wallingford, pp 145–157

    Chapter  Google Scholar 

  • Corral-Martínez P, Seguí-Simarro JM (2012) Efficient production of callus-derived doubled haploids through isolated microspore culture in eggplant (Solanum melongena L.). Euphytica 187:47–61

    Article  Google Scholar 

  • Corral-Martínez P, Seguí-Simarro JM (2014) Refining the method for eggplant microspore culture: effect of abscisic acid, epibrassinolide, polyethylene glycol, naphthaleneacetic acid, 6-benzylaminopurine and arabinogalactan proteins. Euphytica 195:369–382

    Article  Google Scholar 

  • Corral-Martínez P, Parra-Vega V, Seguí-Simarro JM (2013) Novel features of Brassica napus embryogenic microspores revealed by high pressure freezing and freeze substitution: evidence for massive autophagy and excretion-based cytoplasmic cleaning. J Exp Bot 64:3061–3075

    Article  PubMed  Google Scholar 

  • Daghma DES, Hensel G, Rutten T, Melzer M, Kumlehn J (2014) Cellular dynamics during early barley pollen embryogenesis revealed by time-lapse imaging. Front Plant Sci 5:675

    Article  PubMed  PubMed Central  Google Scholar 

  • Doğramacı-Altuntepe M, Peterson TS, Jauhar PP (2001) Anther culture-derived regenerants of durum wheat and their cytological characterization. J Hered 92:56–64

    Article  PubMed  Google Scholar 

  • Dumas de Vaulx R, Chambonnet D (1982) Culture in vitro d’anthères d’aubergine (Solanum melongena L.): stimulation de la production de plantes au moyen de traitements à 35°C associés à de faibles teneurs en substances de croissance. Agronomie 2:983–988

    Article  Google Scholar 

  • Dumas de Vaulx R, Chambonnet D, Pochard E (1981) Culture in vitro d’anthères de piment (Capsicum annuum L.): amèlioration des taux d’obtenction de plantes chez différents génotypes par des traitments à +35°C. Agronomie 1:859–864

    Article  Google Scholar 

  • Eudes F, Shim Y-S, Jiang F (2014) Engineering the haploid genome of microspores. Biocatal Agric Biotechnol 3:20–23

    Google Scholar 

  • FAOSTAT (2016). http://faostat.fao.org. Accessed Dec 2016

  • Ferrie A, Caswell K (2011) Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell Tissue Organ Cult 104:301–309

    Article  Google Scholar 

  • Ferrie AMR, Keller WA (1995) Microspore culture for haploid plant production. In: Gamborg OL, Phillips GC (eds) Plant cell, tissue and organ culture: fundamental methods. Springer, Berlin, pp 155–164

    Chapter  Google Scholar 

  • Ferrie A, Möllers C (2011) Haploids and doubled haploids in Brassica spp. for genetic and genomic research. Plant Cell Tissue Organ Cult 104:375–386

    Article  Google Scholar 

  • Ferrie AMR, Palmer CE, Keller WA (1995) Haploid embryogenesis. In: Thorpe TA (ed) In vitro embryogenesis in plants. Kluwer Academic Publishers, Dordrecht, pp 309–344

    Chapter  Google Scholar 

  • Forster BP, Heberle-Bors E, Kasha KJ, Touraev A (2007) The resurgence of haploids in higher plants. Trends Plant Sci 12:368–375

    Article  CAS  PubMed  Google Scholar 

  • Frary A, Doganlar S, Daunay MC (2007) Eggplant. In: Kole C (ed) Vegetables. Springer, Berlin, pp 287–313

    Chapter  Google Scholar 

  • Germanà MA (2011a) Anther culture for haploid and doubled haploid production. Plant Cell Tissue Organ Cult 104:283–300

    Article  Google Scholar 

  • Germanà MA (2011b) Gametic embryogenesis and haploid technology as valuable support to plant breeding. Plant Cell Rep 30:839–857

    Article  PubMed  Google Scholar 

  • Hoekstra S, Vanzijderveld MH, Louwerse JD, Heidekamp F, Vandermark F (1992) Anther and microspore culture of Hordeum vulgare L. cv. Igri. Plant Sci 86:89–96

    Article  CAS  Google Scholar 

  • Immonen S, Robinson J (2000) Stress treatments and Ficoll for improving green plant regeneration in triticale anther culture. Plant Sci 150:77–84

    Article  CAS  Google Scholar 

  • Kaeppler SM, Kaeppler HF, Rhee Y (2000) Epigenetic aspects of somaclonal variation in plants. Plant Mol Biol 43:179–188

    Article  CAS  PubMed  Google Scholar 

  • Kasha KJ, Simion E, Miner M, Letarte J, Hu TC (2003) Haploid wheat isolated microspore culture protocol. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Doubled haploid production in crop plants. Kluwer Academic Publishers, Dordrecht, pp 77–82

    Chapter  Google Scholar 

  • Li H, Soriano M, Cordewener J, Muiño JM, Riksen T, Fukuoka H, Angenent GC, Boutilier K (2014) The histone deacetylase inhibitor Trichostatin A promotes totipotency in the male gametophyte. Plant Cell 26:195–209

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Malik MR, Wang F, Dirpaul J, Zhou N, Hammerlindl J, Keller W, Abrams SR, Ferrie AMR, Krochko JE (2008) Isolation of an embryogenic line from non-embryogenic Brassica napus cv. Westar through microspore embryogenesis. J Exp Bot 59:2857–2873

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Miah MAA, Earle ED, Khush GS (1985) Inheritance of callus formation ability in anther cultures of rice, Oryza sativa L. Theor Appl Genet 70:113–116

    CAS  PubMed  Google Scholar 

  • Miyoshi K (1996) Callus induction and plantlet formation through culture of isolated microspores of eggplant (Solanum melongena L.). Plant Cell Rep 15:391–395

    Article  CAS  PubMed  Google Scholar 

  • Nitsch JP (1972) Haploid plants from pollen. Z Pflanzenzücht 67:3–18

    Google Scholar 

  • Nitsch C, Nitsch JP (1967) Induction of flowering in vitro in stem segments of Plumbago indica L. I. Production of vegetative buds. Planta 72:355

    Article  CAS  PubMed  Google Scholar 

  • Nuez F, Llácer G (2001) La horticultura española. Ediciones de Horticultura, S.L., Reus

    Google Scholar 

  • Oleszczuk S, Rabiza-Swider J, Zimny J, Lukaszewski AJ (2011) Aneuploidy among androgenic progeny of hexaploid triticale (XTriticosecale Wittmack). Plant Cell Rep 30:575–586

    Article  CAS  PubMed  Google Scholar 

  • Petolino JF, Jones AM, Thompson SA (1988) Selection for increased anther culture response in maize. Theor Appl Genet 76:157–159

    Article  CAS  PubMed  Google Scholar 

  • Quimio CA, Zapata FJ (1990) Diallel analysis of callus induction and green-plant regeneration in rice anther culture. Crop Sci 30:188–192

    Article  Google Scholar 

  • Rivas-Sendra A, Corral-Martínez P, Camacho-Fernández C, Seguí-Simarro JM (2015) Improved regeneration of eggplant doubled haploids from microspore-derived calli through organogenesis. Plant Cell Tissue Organ Cult 122:759–765

    Article  Google Scholar 

  • Rizza F, Mennella G, Collonnier C, Shiachakr D, Kashyap V, Rajam MV, Prestera M, Rotino GL (2002) Androgenic dihaploids from somatic hybrids between Solanum melongena and S. aethiopicum group Gilo as a source of resistance to Fusarium oxysporum f. sp. melongenae. Plant Cell Rep 20:1022–1032

    Article  CAS  Google Scholar 

  • Rotino GL (1996) Haploidy in eggplant. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants. Kluwer Academic Publishers, Dordrecht, pp 115–141

    Chapter  Google Scholar 

  • Rotino GL, Restaino F, Gjomarkaj M, Massimo M, Falavigna A, Schiavi M, Vicini E (1991) Evaluation of genetic variability in embryogenetic and androgenetic lines of eggplant. Acta Hortic 300:357–362

    Google Scholar 

  • Rotino GL, Sihachakr D, Rizza F, Vale G, Tacconi MG, Alberti P, Mennella G, Sabatini E, Toppino L, D’Alessandro A, Acciarri N (2005) Current status in production and utilization of dihaploids from somatic hybrids between eggplant (Solanum melongena L.) and its wild relatives. Acta Physiol Plant 27:723–733

    Article  CAS  Google Scholar 

  • Salas P, Prohens J, Seguí-Simarro JM (2011) Evaluation of androgenic competence through anther culture in common eggplant and related species. Euphytica 182:261–274

    Article  CAS  Google Scholar 

  • Salas P, Rivas-Sendra A, Prohens J, Seguí-Simarro JM (2012) Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures. Euphytica 184:235–250

    Article  Google Scholar 

  • Sanguineti MC, Tuberosa R, Conti S (1990) Field evaluation of androgenetic lines of eggplant. Acta Hortic 280:177–182

    Article  Google Scholar 

  • Seguí-Simarro JM (2016) Androgenesis in Solanaceae. In: Germanà MA, Lambardi M (eds) In vitro embryogenesis. Springer, New York, pp 209–244

    Chapter  Google Scholar 

  • Seguí-Simarro JM, Nuez F (2008) Pathways to doubled haploidy: chromosome doubling during androgenesis. Cytogenet Genome Res 120:358–369

    Article  PubMed  Google Scholar 

  • Seguí-Simarro JM, Corral-Martínez P, Parra-Vega V, González-García B (2011) Androgenesis in recalcitrant solanaceous crops. Plant Cell Rep 30:765–778

    Article  PubMed  Google Scholar 

  • Snape JW, Sitch LA, Simpson E, Parker BB (1988) Tests for the presence of gametoclonal variation in barley and wheat doubled haploids produced using the miah system. Theor Appl Genet 75:509–513

    Article  Google Scholar 

  • Soriano M, Li H, Jacquard C, Angenent GC, Krochko J, Offringa R, Boutilier K (2014) Plasticity in cell division patterns and auxin transport dependency during in vitro embryogenesis in Brassica napus. Plant Cell 26:2568–2581

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Toppino L, Mennella G, Rizza F, D’Alessandro A, Sihachakr D, Rotino GL (2008) ISSR and isozyme characterization of androgenetic dihaploids reveals tetrasomic inheritance in tetraploid somatic hybrids between Solanum melongena and Solanum aethiopicum group Gilo. J Hered 99:304–315

    Article  CAS  PubMed  Google Scholar 

  • Uzun S (2007) Effect of light and temperature on the phenology and maturation of the fruit of eggplant (Solanum melongena) grown in greenhouses. N Z J Crop Hortic Sci 35:51–59

    Article  Google Scholar 

  • van der Weerden GM, Barendse GWM (2007) A web-based searchable database developed for the EGGNET project and applied to the Radboud University Solanaceae database. Acta Hortic 745:503–506

    Article  Google Scholar 

  • Veilleux RE (1998) Gametoclonal variation in crop plants. In: Jain SM, Brar DS, Ahloowalia BS (eds) Somaclonal variation and induced mutations in crop improvement. Springer, Dordrecht, pp 123–133

    Chapter  Google Scholar 

  • Yamagishi M, Yano M, Fukuta Y, Fukui K, Otani M, Shimada T (1996) Distorted segregation of RFLP markers in regenerated plants derived from anther culture of an F1 hybrid of rice. Genes Genet Syst 71:37–41

    Article  CAS  Google Scholar 

  • Zhang FL, Takahata Y (2001) Inheritance of microspore embryogenic ability in Brassica crops. Theor Appl Genet 103:254–258

    Article  CAS  Google Scholar 

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Acknowledgements

We would like to thank the reviewers of this manuscript for their critical and helpful comments. This work was supported by Grant AGL2014-55177-R to JMSS from Spanish Ministerio de Economía y Competitividad (MINECO) jointly funded by FEDER. ARS is supported by a Predoctoral Fellowship from the FPI Program of Universitat Politècnica de València.

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  1. Cell Biology Group, COMAV Institute, Universitat Politècnica de València, Valencia, Spain

    Alba Rivas-Sendra, Manuel Campos-Vega, Antonio Calabuig-Serna & Jose M. Seguí-Simarro

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  1. Alba Rivas-Sendra
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  2. Manuel Campos-Vega
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  3. Antonio Calabuig-Serna
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  4. Jose M. Seguí-Simarro
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Correspondence to Jose M. Seguí-Simarro.

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Rivas-Sendra, A., Campos-Vega, M., Calabuig-Serna, A. et al. Development and characterization of an eggplant (Solanum melongena) doubled haploid population and a doubled haploid line with high androgenic response. Euphytica 213, 89 (2017). https://doi.org/10.1007/s10681-017-1879-3

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