Euphytica

, 213:98

QTL analysis of resistance to powdery mildew in hop (Humulus lupulus L.)

  • J. A. Henning
  • D. H. Gent
  • M. S. Townsend
  • J. L. Woods
  • S. T. Hill
  • D. Hendrix
Article

DOI: 10.1007/s10681-017-1849-9

Cite this article as:
Henning, J.A., Gent, D.H., Townsend, M.S. et al. Euphytica (2017) 213: 98. doi:10.1007/s10681-017-1849-9
  • 165 Downloads

Abstract

Hop with powdery mildew [HPM: caused by Podosphaera macularis (Wallr.) U. Braun & S. Takam.] results in significant losses in hop production by reducing yield and quality. One means of increasing yield and quality is the production of resistant hop lines. Breeding for resistance can be significantly improved and accelerated by use of marker-assisted selection. The objective of this preliminary study was to identify QTLs and markers for genetic resistance to HPM. A bi-parental mapping population between the resistant line “Newport” and susceptible line ‘21110M’. Phenotypic data was scored under controlled greenhouse conditions. Significant differences among offspring were observed and disease resistance did not follow a distinct binomial distribution, suggesting quantitative genetic control. Genotyping-by-sequencing resulted in approximately 375 K SNP markers, which were filtered down to 2263 markers mapped to 10 linkage groups. Interval Mapping identified four QTLs with one on linkage group 1 and three located on linkage group 6. Composite interval mapping identified three QTLs, all located on linkage group 6. Mixed linear models identified 15 markers associated with expression of resistance to HPM. Three of these 15 SNPs were also identified in QTL-CIM analysis. Evaluation of the scaffolds containing the significant SNP markers identified seven putative genes—several of which appear involved in disease resistance in other plant species. The SNP markers identified in this study still require validation in unrelated populations prior to implementation in breeding programs.

Keywords

Disease resistance Genetic mapping Genome Hop Humulus Powdery Mildew QTL 

Supplementary material

10681_2017_1849_MOESM1_ESM.xlsx (828 kb)
Supplementary material 1 (XLSX 828 kb)
10681_2017_1849_MOESM2_ESM.docx (13 kb)
Supplementary material 2 (DOCX 13 kb)
10681_2017_1849_MOESM3_ESM.xls (32 kb)
Supplementary material 3 (XLS 33 kb)

Funding information

Funder NameGrant NumberFunding Note
Agricultural Research Service
  • CRIS #5358-21000-046-00D
Hop Research Foundation
    The Hop Breeding Company

      Copyright information

      © Springer Science+Business Media Dordrecht (outside the USA) 2017

      Authors and Affiliations

      1. 1.USDA-ARSCorvallisUSA
      2. 2.Department of Crop and Soil SciencesOregon State UniversityCorvallisUSA
      3. 3.Department of Botany and Plant PathologyOregon State UniversityCorvallisUSA
      4. 4.Department of Computer SciencesOregon State UniversityCorvallisUSA
      5. 5.Department of Biochemistry and BiophysicsOregon State UniversityCorvallisUSA

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