Euphytica

, 213:20 | Cite as

Genetics of resistance in lettuce to races 1 and 2 of Verticillium dahliae from different host species

  • German V. Sandoya
  • Suraj Gurung
  • Dylan P. Short
  • Krishna V. Subbarao
  • Richard W. Michelmore
  • Ryan J. Hayes
Article

Abstract

Race 1 resistance against Verticillium dahliae in lettuce was originally shown in the cultivar La Brillante to be conditioned by a single dominant gene (Verticillium resistance 1, Vr1). Multiple, morphologically diverse sources of germplasm have been identified as resistant to race 1. In this study, allelism tests indicated that resistance in these different lettuce cultivars is closely linked or allelic to the Vr1 gene. The Vr1 gene is defeated by race 2 isolates of V. dahliae. Only partial resistance to race 2 isolates is available in a few plant introductions (PIs). Greenhouse and field experiments conducted with these PIs demonstrated partial resistance to V. dahliae race 1 as well as race 2 isolates from lettuce. Cultivars resistant to race 1 and PIs with partial resistance to race 2 were challenged with several race 1 and 2 isolates originating from hosts other than lettuce. This indicated that cultivars resistant to race 1 and the breeding lines derived from them would also be resistant to race 1 isolates from other hosts; similarly, the partial resistance would be effective against race 1 and 2 isolates from hosts other than lettuce. Nevertheless, there were specific interactions that warrant further study. Although race 1 currently predominates in the major lettuce production area of the Salinas Valley, CA, breeding lettuce for resistance to V. dahliae should take both races into account.

Keywords

Breeding Disease Disease resistance Diversity Genetic Host-pathogen interaction Lactuca sativa Vegetable Verticillium wilt 

References

  1. Anonymus (2014) Monterey County Crop Report. Vol. 2016. County of Monterey Agricultural CommissionerGoogle Scholar
  2. Atallah ZK, Hayes RJ, Subbarao KV (2011) Fifteen years of verticillium wilt Of lettuce in America’s salad bowl: a tale of immigration, subjugation, and abatement. Plant Dis 95:784–792CrossRefGoogle Scholar
  3. Bhat RG, Subbarao KV (1999) Host range specificity in Verticillium dahliae. Phytopathology 89:1218–1225CrossRefPubMedGoogle Scholar
  4. Brunner E, Domhof S, Langer F (2002) Nonparametric analysis of longitudinal data in factorial experiments. Wiley, New YorkGoogle Scholar
  5. de Jonge R, van Esse HP, Maruthachalam K, Bolton MD, Santhanam P, Saber MK, Zhang Z, Usami T, Lievens B, Subbarao KV, Thomma B (2012) Tomato immune receptor Ve1 recognizes effector of multiple fungal pathogens uncovered by genome and RNA sequencing. Proc Natl Acad Sci USA 109:5110–5115CrossRefPubMedPubMedCentralGoogle Scholar
  6. Gurung S, Short DPG, Atallah ZK, Subbarao KV (2014) Clonal expansion of Verticillium dahliae in lettuce. Phytopathology 104:641–649CrossRefPubMedGoogle Scholar
  7. Gurung S, Short DPG, Hu X, Sandoya GV, Hayes RJ, Koike ST, Subbarao KV (2015) Host range of Verticillium isaacii and Verticillium klebahnii from artichoke, spinach, and lettuce. Plant Dis 99:933–938CrossRefGoogle Scholar
  8. Hayes RJ, Vallad GE, Qin Q-M, Grube RC, Subbarao KV (2007) Variation for resistance to verticillium wilt in lettuce (Lactuca sativa L.). Plant Dis 91:439–445CrossRefGoogle Scholar
  9. Hayes RJ, Maruthachalam K, Vallad GE, Klosterman SJ, Simko I, Luo YG, Subbarao KV (2011a) Iceberg lettuce breeding lines with resistance to verticillium wilt caused by race 1 isolates of Verticillium dahliae. HortScience 46:501–504Google Scholar
  10. Hayes RJ, Maruthachalam K, Vallad GE, Klosterman SJ, Subbarao KV (2011b) Selection for resistance to verticillium wilt caused by Race 2 isolates of Verticillium dahliae in accessions of lettuce (Lactuca sativa L.). HortScience 46:201–206Google Scholar
  11. Hayes RJ, McHale LK, Vallad GE, Truco MJ, Michelmore RW, Klosterman SJ, Maruthachalam K, Subbarao KV (2011c) The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante. Theor Appl Genet 123:509–517CrossRefPubMedGoogle Scholar
  12. Inderbitzin P, Bostock RM, Davis RM, Usami T, Platt HW, Subbarao KV (2011) Phylogenetics and taxonomy of the fungal vascular wilt pathogen Verticillium, with the descriptions of five new species. PLoS ONE 6:e28341CrossRefPubMedPubMedCentralGoogle Scholar
  13. Jansky S, Rouse DI, Kauth PJ (2004) Inheritance of resistance to Verticillium dahliae in diploid interspecific potato hybrids. Plant Dis 88:1075–1078CrossRefGoogle Scholar
  14. Kabir Z, Bhat RG, Subbarao KV (2004) Comparison of Media for Recovery of Verticillium dahliae from Soil. Plant Dis 88:49–55CrossRefGoogle Scholar
  15. Maruthachalam K, Atallah ZK, Vallad GE, Klosterman SJ, Hayes RJ, Davis RM, Subbarao KV (2010) Molecular variation among isolates of Verticillium dahliae and polymerase chain reaction-based differentiation of races. Phytopathology 100:1222–1230CrossRefPubMedGoogle Scholar
  16. Mert M, Kurt S, Gencer O, Akiscan Y, Boyaci K, Tok FM (2005) Inheritance of resistance to verticillium WILT (Verticillium dahliae) in cotton (Gossypium hirsutum L.). Plant Breeding 124:102–104CrossRefGoogle Scholar
  17. Pegg GF, Brady BL (2002) Verticillium wilts. CABI Publishing, New YorkCrossRefGoogle Scholar
  18. Ryder EJ (1999) Lettuce, endive and chicory. Crop production science in horticulture series. CABI Publishing, New YorkGoogle Scholar
  19. Shah DA, Madden LV (2004) Nonparametric analysis of ordinal data in designed factorial experiments. Phytopathology 94:33–43CrossRefPubMedGoogle Scholar
  20. Short DPG, Gurung S, Maruthachalam K, Atallah ZK, Subbarao KV (2014) Verticillium dahliae race 2-specific PCR reveals a high frequency of race 2 strains in commercial spinach seed lots and delineates Race structure. Phytopathology 104:779–785CrossRefPubMedGoogle Scholar
  21. Short DPG, Sandoya G, Vallad GE, Koike ST, Xiao C-L, Wu B-M, Gurung S, Hayes RJ, Subbarao KV (2015) Dynamics of verticillium species microsclerotia in field soils in response to fumigation, cropping patterns, and flooding. Phytopathology 105:638–645CrossRefPubMedGoogle Scholar
  22. Subbarao KV, Hubbard JC, Greathead AS (1997) Verticillium wilt. In: Davis RM, Subbarao KV, Raid RN, Kurtz EA (eds) Compendium of lettuce diseases, vol 26–27. The American Phytopathological Society, St. PaulGoogle Scholar
  23. Vallad GE, Qin Q-M, Subbarao KV (2004) Verticillium wilt of cool season vegetable crops: their distribution, impact, and management. Recent research and developments of plant pathology, vol 3. Research Signpost, Trivandrum, pp 189–210Google Scholar
  24. Vallad GE, Qin QM, Grube R, Hayes RJ, Subbarao KV (2006) Characterization of race-specific interactions among isolates of Verticillium dahliae pathogenic on lettuce. Phytopathology 96:1380–1387CrossRefPubMedGoogle Scholar
  25. Ye G, Smith KF (2008) Marker-assisted gene pyramiding for inbred line development: basic principles and practical guidelines. Int J Plant Breed 2:1–10CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht (outside the USA) 2017

Authors and Affiliations

  • German V. Sandoya
    • 1
    • 2
  • Suraj Gurung
    • 3
    • 4
  • Dylan P. Short
    • 3
    • 5
  • Krishna V. Subbarao
    • 3
  • Richard W. Michelmore
    • 1
    • 2
  • Ryan J. Hayes
    • 6
  1. 1.The Genome Center and Department of Plant SciencesUniversity of California, DavisDavisUSA
  2. 2.Everglades Research Education Center, Horticultural Sciences Department – IFASUniversity of FloridaBelle GladeUSA
  3. 3.Department of Plant PathologyUniversity of California, DavisSalinasUSA
  4. 4.Sakata Seed of AmericaSalinasUSA
  5. 5.Division of Plant and Soil SciencesWest Virginia UniversityMorgantownUSA
  6. 6.National Forage Seed Production Research CenterUnited States Department of Agriculture, USDACorvallisUSA

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