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Euphytica

, Volume 212, Issue 3, pp 501–514 | Cite as

Chromosome markers confirm origin of Heliconia hybrids and triploids

  • Marília Gabriela de Santana Costa
  • Brunno Sérgio Ferreira Leite
  • Vivian Loges
  • Ebenézer Bernardes Correia Silva
  • Andreza Santos da Costa
  • Walma Nogueira Ramos Guimarães
  • Ana Christina Brasileiro-Vidal
Article

Abstract

Heliconia L., the only genus in the Heliconiaceae family, comprises about 380 species with high ornamental potential. However, the identification of genotypes—mainly based on morphological characteristics—has generated synonyms. Therefore, it becomes necessary to apply novel techniques to differentiate genotypes and elucidate the origin of hybrids and triploids. The aim of the present work was the cytogenetic characterization of 18 Heliconia genotypes, including three hybrids, two triploids and their possible parent plants, by means of chromosome counting, staining with the fluorochromes CMA and DAPI, and FISH for 45S and 5S rDNA sites. The analyses revealed 16 diploid (2n = 2x = 24) and two triploid genotypes (2n = 3x = 36), with new counts for eight genotypes, including the triploid ‘Suriname Sassy’. The karyotypes were symmetric, with chromosomes varying from 0.88 to 3.35 µm. Regarding the CMA/DAPI staining and FISH, one CMA+/DAPI0/5S rDNA band was observed in the proximal region of two and three small chromosomes in the diploid and triploid genotypes, respectively. The CMA++/DAPI/45S rDNA markings varied from two to four sites per genotype, revealing some interesting heteromorphisms. The distribution of 5S and 45S rDNA sites, both CMA-positive, corroborated the origin of the triploid genotypes ‘Sassy’ and ‘Suriname Sassy’ from diploid genotypes of H. psittacorum, as well as the origin of the hybrids of H. psittacorum × H. spathocircinata (‘Golden Torch’ and ‘Golden Torch Adrian’) and H. caribaea × H. bihai cv. ‘Jacquinii’ from their respective parent species.

Keywords

45S rDNA Chromosome makers CMA/DAPI FISH Hybrids Triploids 

Notes

Acknowledgments

The authors would like to thank the research funding organs CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) and CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) for financial support, and the IAC (Instituto Agronômico de Campinas), in the person of Dr. Carlos Castro, for providing rhizomes of H. spathocircinata, Dr. Marcelo Guerra, for allowing the use of his laboratory, Dr. Ana Maria Benko Iseppon, for her suggestions and the Bem-Te-Vi Farm.

Supplementary material

10681_2016_1780_MOESM1_ESM.tif (12.2 mb)
Online Resource 1 Metaphases of Heliconia genotypes stained with the fluorochromes CMA (yellow) and DAPI (pseudo-colored in grey): (a) H. latispatha Bentham cv. ‘Distans’; (b) H. stricta Huber; (c) H. stricta Huber cv. ‘Fire Bird’; (d) H. wagneriana Petersen; (e) H. collinsiana Griggs; (f) H. pendula Wawra; (g) H. rauliniana Barreiros; and (h) H. rostrata Ruiz & Pavón. Bar in h corresponds to 10 µm. Supplementary material 1 (TIFF 12527 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Marília Gabriela de Santana Costa
    • 1
  • Brunno Sérgio Ferreira Leite
    • 2
  • Vivian Loges
    • 1
  • Ebenézer Bernardes Correia Silva
    • 3
  • Andreza Santos da Costa
    • 1
  • Walma Nogueira Ramos Guimarães
    • 1
    • 4
  • Ana Christina Brasileiro-Vidal
    • 1
    • 2
  1. 1.Departamento de Agronomia da Universidade Federal Rural de Pernambuco (UFRPE)RecifeBrazil
  2. 2.Departamento de Genética da Universidade Federal de Pernambuco (UFPE)RecifeBrazil
  3. 3.Departamento de Ciências da Natureza do Instituto Federal de Alagoas (IFAL)MaceióBrazil
  4. 4.Departamento de Biologia da Universidade Federal Rural de Pernambuco (UFRPE)RecifeBrazil

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