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Main effect QTLs associated with arsenic phyto-toxicity tolerance at seedling stage in rice (Oryza sativa L.)

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Abstract

Arsenic phyto-toxicity is a limiting factor affecting rice production in the Indo-Gangetic flood plain. In this study, 98 microsatellite markers were used to map quantitative trait loci (QTL) conferring arsenic phyto-toxicity tolerance (AsT) at seedling stage from a F2 population derived from a cross between an arsenic sensitive indica rice cultivar (BRRI dhan45) and an arsenic tolerant indica rice cultivar (BRRI dhan47) using selective genotyping technique. Arsenic phyto-toxicity tolerance of F2:3 population was assessed with an arsenic stress of 1.25 mg L−1 under hydroponic culture in a climate chamber. AsT indices for shoot length, root length and root-shoot biomass were estimated in terms of comparative growth in treated plants compared to that in control plants. A total of four main effect QTLs with significant LOD comprising two for relative growth in shoot length, one for each of root length and root-shoot biomass under arsenic stress were identified on chromosomes 8 and 12 using composite interval mapping approach. A large effect QTL for arsenic phyto-toxicity tolerance in root length contributing 24.9 % phenotypic variation was identified on chromosome 8 with plausible pleiotropic effect on shoot length and root-shoot biomass.

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Acknowledgments

The authors gratefully acknowledge financial support for this research from the United States Dept. of Agriculture Food for Progress Program at Cornell University (FCC-388-2009) and administrative assistance from Bangabandhu Sheikh Mujibur Rahman Agricultural University.

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Authors and Affiliations

  1. Plant Breeding Division, Bangladesh Rice Research Institute, Gazipur, 1701, Bangladesh

    Md. Abu Syed, K. M. Iftekharuddaula & Partha S. Biswas

  2. Bangabandhu Sheikh Mujibur Rahman, Agricultural University, Gazipur, 1706, Bangladesh

    Md. Abu Syed, M. A. Khaleque Mian, Md. Golam Rasul & G. K. M. Mustafizur Rahmam

  3. Former Director Research, Bangladesh Rice Research Institute, Gazipur, 1701, Bangladesh

    Golam M. Panaullah

  4. Department of Crop and Soil Sciences, Cornell University, Ithaca, NY, 14850, USA

    Julie G. Lauren & John M. Duxbury

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  1. Md. Abu Syed
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  2. K. M. Iftekharuddaula
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  3. M. A. Khaleque Mian
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Correspondence to Partha S. Biswas.

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Syed, M.A., Iftekharuddaula, K.M., Mian, M.A.K. et al. Main effect QTLs associated with arsenic phyto-toxicity tolerance at seedling stage in rice (Oryza sativa L.). Euphytica 209, 805–814 (2016). https://doi.org/10.1007/s10681-016-1683-5

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