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Euphytica

, Volume 208, Issue 2, pp 225–236 | Cite as

Effects of mild drought stress on the morpho-physiological characteristics of a bambara groundnut segregating population

  • Hui Hui Chai
  • Festo Massawe
  • Sean Mayes
Article

Abstract

Bambara groundnut (Vigna subterranea (L) Verdc.) is a drought tolerant underutilised indigenous African legume. The present study aimed to examine the response of bambara groundnut under increasing drought stress and the effects of cumulative mild drought on final yields. The components of morpho-physiological traits were measured for a small F5 breeding cross of bambara groundnut exposed to progressive mild drought in controlled-environment tropical glasshouses. Drought stress reduced stomatal conductance significantly (F (1,130) = 2259.59, p < 0.01), with variation observed between lines of the segregating population (F (64,130) = 16.27, p < 0.01). Higher stomatal density and reduced leaf area were observed in drought treatment plants (p < 0.01). Mild drought stress negatively influenced 100-seed weight (F (1,258) = 19.4, p < 0.01) and harvest index (F (1,258) = 12.87, p < 0.01) by 8 and 15.6 %, compared to the control irrigated treatment, respectively. Bambara groundnut used a combination of mechanisms to tolerate drought stress, including stomatal regulation of gas exchange, reduction of leaf area and maintenance of a relatively high leaf water status and relatively high levels of photosynthesis. Strong genotypic variation observed for many traits in the F5 segregating population allows individual lines with potentially greater tolerance for drought, combined with higher yielding characteristics, to be selected for future breeding programmes in bambara groundnut.

Keywords

Breeding Genetic variation Mild drought stress Plant growth Stomatal conductance Stomatal density 

Notes

Acknowledgments

This study was supported by the University of Nottingham Malaysia Campus under Malaysia Intercampus Doctoral Award Scheme (MIDAS).

Supplementary material

10681_2015_1581_MOESM1_ESM.docx (713 kb)
Supplementary material 1 (DOCX 712 kb)

References

  1. Ahmad NS (2012) Genetic analysis of plant morphology in bambara groundnut (Vigna subterranea (L.) Verdc.). PhD thesis, University of Nottingham, NottinghamGoogle Scholar
  2. Ahmed FE, Suliman ASH (2010) Effect of water stress applied at different stages of growth on seed yield and water-use efficiency of Cowpea. Agric Biol J N Am 1:534–540Google Scholar
  3. Anyia AO, Herzog H (2004) Genotypic variability in drought performance and recovery in Cowpea under controlled environment. J Agron Crop Sci 190:151–159CrossRefGoogle Scholar
  4. Araus JL, Bort J, Tambussi EA (2007) Water use efficiency in C3 cereals under Mediterranean conditions: a review of some physiological aspects. In: Lamaddalena N, Shatanawi M, Todorovic M, Bogliotti C, Albrizio R (eds) Water use efficiency and water productivity: WASAMED project. CIHEAM, Bari, pp 189–203Google Scholar
  5. Babekir AM (1989) Growth, dry matter and yield of bambara groundnut (V. subterranea) and groundnut (A. hypogaea) under irrigated and droughted conditions. MSc thesis, University of Nottingham, NottinghamGoogle Scholar
  6. Baigorri H, Antolin MC, Sanchez-Diaz M (1999) Reproductive responses of two morphologically different pea cultivars to drought. Eur J Agron 10:119–128CrossRefGoogle Scholar
  7. Begemann F (1988) Ecogeographic differentiation of bambara groundnut (Vigna subterranea) in the collection of the International Institute of Tropical Agriculture (IITA). Wissenschaftlicher Fachverlag, Giessen, p 153Google Scholar
  8. Berchie JN, Opoku M, Adu-Dapaah H, Agyemang A, Sarkodie-Addo J, Asare E, Addo J, Akuffo H (2012) Evaluation of five bambara groundnut (Vigna subterranea (L.) Verdc.) landraces to heat and drought stress at Tono-Navrongo, Upper East region of Ghana. Afr J Agric Res 7:250–256CrossRefGoogle Scholar
  9. Blair MW, Galeano CH, Tovar E, Torres MCM, Castrillon AV, Beebe SE, Rao IM (2012) Development of a Mesoamerican intra-genepool genetic map for quantitative trait loci detection in a drought tolerant x susceptible common bean (Phaseolus vulgaris L.) cross. Mol Breed 29:71–88PubMedCentralCrossRefPubMedGoogle Scholar
  10. Burgess J (2006) Country pasture/forage resource profiles—Botswana. FAO, RomeGoogle Scholar
  11. Cattivelli L, Di Fonzo N, Mastrangelo AM, Mazzucco L, Rascio A, Russo M (2000) Molecular aspects of abiotic stress resistance in durum wheat. In: Royo C, Nachit M, Di Fonzo N, Araus JL (eds) Durum wheat improvement in the Mediterranean region: new challenges. CIHEAM, Zaragoza, pp 207–213Google Scholar
  12. Collino DJ, Dardanelli JL, Sereno R, Racca RW (2001) Physiological responses of argentine peanut varieties to water stress—light interception, radiation use efficiency and partitioning of assimilates. Field Crops Res 70:177–184CrossRefGoogle Scholar
  13. Collinson ST, Clawson EJ, Azam-Ali SN, Black CR (1997) Effects of soil moisture deficits on water relations of bambara groundnut (Vigna subterranea L. Verdc.). J Exp Bot 48:877–884CrossRefGoogle Scholar
  14. Datta SC (1998) Plant physiology. New Age International, New Delhi, p 104Google Scholar
  15. Duke JA (1981) Handbook of legumes of world economic importance. Plenum Press, New York, pp 307–310CrossRefGoogle Scholar
  16. Ebdon JS, Kopp KL (2004) Relationships between water use efficiency, carbon isotope discrimination, and turf performance in genotypes of Kentucky bluegrass during drought. Crop Sci 44:1754–1762CrossRefGoogle Scholar
  17. El-Sharkawy MA, Cock JH, Hernandez ADP (1985) Stomatal response to air humidity and its relation to stomatal density in a wide range of warm climate species. Photosynth Res 7:137–149CrossRefPubMedGoogle Scholar
  18. Farquhar GD, Ehleringer JR, Hubick T (1989) Carbon isotope discrimination and photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 40:503–537CrossRefGoogle Scholar
  19. Food and Agriculture Organization of the United Nations (FAO) (2009) FAOStat. Food and Agriculture Organisation of the United Nations, Rome. http://faostat.fao.org/default.aspx. Accessed 26 Mar 2013
  20. Hall AE, Patel PN (1985) Breeding for resistance to drought and heat. In: Singh SR, Rachie KO (eds) Cowpea research, production and utilization. Wiley, New York, pp 137–151Google Scholar
  21. Heller J, Begemann F, Mushonga J (1997) Bambara groundnut (Vigna subterranea (L.) Verdc.). In: Promoting the conservation and use of underutilized and neglected crops (ed) 9. Proceedings of the workshop on Conservation and Improvement of Bambara Groundnut (Vigna subterranea (L.) Verdc.), 14–16 Nov 1995, Harare, Zimbabwe, International Plant Genetic Resources Institute (IPGRI), RomeGoogle Scholar
  22. IPGRI (2000) Descriptors for bambara groundnut (Vigna subterranea). International Plant Genetic Resources Institute, Rome; International Institute of Tropical Agriculture, Ibadan; The International Bambara Groundnut Network, HamburgGoogle Scholar
  23. Jorgensen ST, Liu F, Ouedraogo M, Ntundu WH, Sarrazin J, Christiansen JL (2010) Drought responses of two bambara groundnut (Vigna subterannea L. Verdc.) landraces collected from a dry and a humid area of Africa. J Agron Crop Sci 196:412–422CrossRefGoogle Scholar
  24. Jorgensen ST, Ntundu WH, Ouedraogo M, Christiansen JL, Liu F (2011) Effect of a short and severe intermittent drought on transpiration, seed yield, yield components, and harvest index in four landraces of bambara groundnut. Int J Plant Prod 5:25–36Google Scholar
  25. Kgathi DL, Mazonde I, Murray-Hudson M (2012) Water implications of biofuel development in semi-arid sub-saharan Africa: case studies for four Countries. In: Janssen R, Rutz D (eds) Bioenergy for sustainable development in Africa. Springer, New York, pp 261–280CrossRefGoogle Scholar
  26. Kumawat G, Raje RS, Bhutani S, Pal JK, Mithra ASVRC, Gaikwad K, Sharma TR, Singh NK (2012) Molecular mapping of QTLs for plant type and earliness traits in pigeonpea (Cajanus cajan L. Millsp.). BMC Genet 13:84–95PubMedCentralCrossRefPubMedGoogle Scholar
  27. Liu F, Andersen MN, Jensen CR (2003) Loss of pot set caused by drought stress is associated with water status and ABA content of reproductive structures in soybean. Funct Plant Biol 30:271–280CrossRefGoogle Scholar
  28. Mabhaudhi T, Modi AT, Beletse YG (2013) Growth, phenological and yield responses of a bambara groundnut (Vigna subterranea L. Verdc) landrace to imposed water stress: II. Rain shelter conditions. Water SA 39:191–198Google Scholar
  29. Massawe FJ, Mwale SS, Azam-Ali SN, Roberts JA (2005) Breeding in bambara groundnut (Vigna subterranea (L) Verdc.): Strategic considerations. Afr J Biotech 4:463–471Google Scholar
  30. Meng L, Li L, Chen W, Xu Z, Liu L (1999) Effect of water stress and temperature on leaf size and number of epidermal cells in grain sorghum. Crop Sci 14:751–755Google Scholar
  31. Mohammadkhani N, Heidari R (2008) Drought-induced accumulation of soluble sugar and proline in two maize varieties. World Appl Sci J 3:448–453Google Scholar
  32. Mwale SS, Azam-Ali SN, Massawe FJ (2007) Growth and development of bambara groundnut (Vigna subterranea) in response to soil moisture 1. Dry matter and yield. Eur J Agron 26:345–353CrossRefGoogle Scholar
  33. National Academy of Sciences (NAS) (1979) Bambara groundnuts. In: Rachie KO (ed) Tropical legumes, resources for the future. National Academy of Science, Washington DC, pp 47–53Google Scholar
  34. Okcu G, Kaya MD, Atak M (2005) Effects of salt and drought stresses on germination and seedling growth of pea (Pisum sativum L.). Turk J Agric For 29:237–242Google Scholar
  35. Pedercini M, Kanamaru H, Derwisch S (2012) Potential impacts of climate change on food security in Mali. Natural Resources Management and Environment Department,FAO, RomeGoogle Scholar
  36. Rachie KO, Roberts LM (1974) Grain legumes of the lowland tropics. Adv Agron 26:355Google Scholar
  37. Sapeta H, Costa JM, Lourenco T, Maroco J, Linde PVD, Oliveira MM (2013) Drought stress response in Jatropha curcas: growth and physiology. Environ Exp Bot 85:76–84CrossRefGoogle Scholar
  38. Sellschope JPF (1962) Cowpeas, Vigna unguiculata (L.) walp. Field Crop Abstr 15:259–266Google Scholar
  39. Singh P (1991) Influence of water deficits on phenology, growth and dry matter allocation in chickpea (Cicer arietinum). Field Crops Res 28:1–15CrossRefGoogle Scholar
  40. Somal TLC, Yapa PAJ (1998) Accumulation of proline in Cowpea under nutrient, drought and saline stresses. J Plant Nutr 21:2465–2473CrossRefGoogle Scholar
  41. Songsri P, Jogloy S, Junjittakarn J, Kesmala T, Vorasoot N, Holbrook CC, Patanothai A (2013) Association of stomatal conductance and root distribution with water use efficiency of peanut under different soil water regimes. Aust J Crop Sci 7:948–955Google Scholar
  42. Sprent J (2009) Legume nodulation: a global perspective. Wiley Blackwell, Chichester, p 99CrossRefGoogle Scholar
  43. Stadler F (2009) Analysis of differential gene expression under water-deficit stress and genetic diversity in bambara groundnut (Vigna subterranea (L.) Verdc.) using novel high-throughput technologies. PhD Thesis, Technical University of Munich, MunichGoogle Scholar
  44. Tilahun A, Schubert S (2003) Mechanisms of drought resistance in grain: II Stomatal regulation and root growth. SINET Ethiop J Sci 26:137–144Google Scholar
  45. VSN International (2012) Genstat for Windows, 15th edn. VSN International, Hemel Hempstead. Web page: Genstat.co.uk. Assessed 3 November 2013Google Scholar
  46. Vurayai R, Emongor V, Moseki B (2011) Physiological responses of bambara groundnut (Vigna subterranea (L.) Verdc) to short periods of water stress during different development stages. Asian J Agric Sci 3:37–43Google Scholar
  47. Xu ZH, Zhou GS (2008) Reponses of leaf stomatal density to water status and its relationship with photosynthesis in a grass. J Exp Bot 59:3317–3325PubMedCentralCrossRefPubMedGoogle Scholar
  48. Zhu Q (2002) Salt and drought stress signal transduction in plants. Annu Rev Plant Biol 53:247–273PubMedCentralCrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Biotechnology Research Centre, School of BiosciencesUniversity of NottinghamSemenyihMalaysia
  2. 2.Crops For the FutureSemenyihMalaysia
  3. 3.Plant and Crop Sciences, School of BiosciencesUniversity of NottinghamLoughboroughUK

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