Production and characterization of alien chromosome addition lines in Allium fistulosum carrying extra chromosomes of Allium roylei using molecular and cytogenetic analyses
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Allium roylei was employed for the production of alien chromosome addition lines in A. fistulosum. Interspecific hybridization between A. fistulosum and A. roylei successfully produced F1 hybrids. Chromosome doubling of an F1 hybrid was carried out to produce amphidiploids. After two times backcrossing of the amphidiploids with A. fistulosum, a BC2 generation was obtained with chromosome numbers (2n) ranging from 16 to 23. Alien monosomic addition lines (AMAL, FF + nR, 2n = 17) appeared with the highest frequency. Furthermore, multiple addition lines (MAL, 2n = 18–23) were also observed with lower frequencies. Five AMALs (FF + 1R, +3R, +4R, +5R, and +8R) and ten MALs (2n = 18–23) were characterized using isozyme and DNA markers. The extra chromosomes from A. roylei clearly altered the biochemical characteristics of the MALs. Variations in sugar, cysteine sulfoxide, and flavonoid contents were observed among the MALs in various amounts. Allium fistulosum–A. roylei allotriploids (2n = 24, FFR) showed significantly higher saponin content and antifungal activities of saponin extracts against isolates of Fusarium oxysporum f. sp. cepae in comparison with A. fistulosum. This first report of A. fistulosum–A. roylei addition lines opens the possibility of developing novel A. fistulosum cultivars with enhanced nutritional value and disease resistance.
KeywordsAllium fistulosum Allium roylei Alien addition lines Antifungal activity Biochemical variation
The authors are very thankful to Dr. Shigenori Yaguchi from the Department of Food Science and Technology, National Fisheries University; Dr. Shin-ichi Masuzaki from Forensic Science Laboratory, Yamaguchi Prefectural Police Headquarters; Takane Furuta from Laboratory of Plant Chromosome and Gene Stock, Faculty of Science Hiroshima University and Tomoko Morita for their contribution to this study.
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