Skip to main content
SpringerLink
Log in

Induction of female 2n gametes and creation of tetraploids through sexual hybridization in cassava (Manihot esculenta)

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

In order to exploit the induced female 2n gametes for cassava (Manihot esculenta) breeding, a detailed histological observation of female gametogenesis was conducted to correlate with the morphological characteristics of inflorescences and female flower buds. The SC5 gynoecia at the stage of prophase I were selected for unreduced megaspores induction with 0.3 % colchicine and 1 % dimethyl sulfoxide-soaked cotton plugs for 2 days. The resulted female cyathia were artificially pollinated with ploidy-level mixed pollens of SC10. Out of 250 hybrid progenies, two plants which exhibited morphological variations were confirmed as sexual tetraploids by flow cytometry and chromosome counting. Our results demonstrated that induction of 2n gametes combined with hybridization can be an effective approach for sexual tetraploid production for cassava.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Alves AGC (2001) Cassava botany and physiology. In: Hillocks RJ, Thresh JM, Bellotti AC (eds) Cassava biology, production and utilization. CABI, Wallingford, pp 67–90

    Google Scholar 

  • Barcaccia G, Tavoletti S, Mariani A, Veronesi F (2003) Occurrence, inheritance and use of reproductive mutants in alfalfa improvement. Euphytica 133:37–56

    Article  Google Scholar 

  • Bonierbale M, Guevara C, Dixon AGO, Ng NQ, Asiedu R, Ng SYC (1997) Cassava. In: Fuccillo D, Sears L, Stapleton P (eds) Biodiversity in trust. Cambridge University Press, Cambridge, pp 1–20

    Google Scholar 

  • Bretagnolle F, Thompson JD (1995) Gametes with the somatic chromosome number: mechanisms of their formation and role in the evolution of autopolyploid plants. New Phytol 129:1–22

    Article  Google Scholar 

  • Brownfield L, Köhler C (2010) Unreduced gamete formation in plants: mechanisms and prospects. J Exp Bot 62:1659–1668

    Article  PubMed  Google Scholar 

  • Ceballos H, Okogbenin E, Pérez JC, Becerra LA, Debouck D (2010) Cassava. In: Bradshaw J (ed) Root and tuber crops. Springer, New York, pp 53–96

    Chapter  Google Scholar 

  • Cock JH (1982) Cassava: a basic energy source in the tropics. Science 218(4574):755–762

    Article  CAS  PubMed  Google Scholar 

  • Consiglio F, Carputo D, Monti L, Conicella C (2004) Exploitation of genes affecting meiotic non-reduction and nuclear restitution: Arabidopsis as a model? Sex Plant Reprod 17:97–105

    Article  Google Scholar 

  • De-Paula OC, Sajo MG (2011) Morphology and development of anthers and ovules in Croton and Astraea (Euphorbiaceae). Nord J Bot 29(4):505–511

    Article  Google Scholar 

  • Dewitte A, Van Laere K, Van Huylenbroeck J (2011) Use of 2n gametes in plant breeding. In: Abdurakhmonov IY (ed) Plant breeding. InTech Open Access Publisher, Croatia, pp 59–86

    Google Scholar 

  • Galbraith DH, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E (1983) Rapid flow cytometric analysis of the cell cycle in intact plant tissue. Science 220:1049–1051

  • Gallo PH, Micheletti PL, Boldrini KR, Risso-Pascotto C, Pagliarini MS, Valle CB (2007) 2n gamete formation in the genus Brachiaria (Poaceae: Paniceae). Euphytica 154:255–260

    Article  Google Scholar 

  • Gleadow RM, Evans JR, Mccaffery S, Cavagnaro TR (2009) Growth and nutritive value of cassava (Manihot esculenta Cranz.) are reduced when grown in elevated CO2. Plant Biol 11:76–82

    Article  CAS  PubMed  Google Scholar 

  • Hahn SK, Bai KV, Chukwuma R, Dixon AA, Ng SY (1991) Polyploidy breeding of cassava. In: Proceedings of the 9th symposium of the international society for tropical root crops, pp 102–109

  • Jennings DL, Iglesias C (2001) Breeding for crop improvement. In: Hillocks RJ, Thresh JM, Bellotti AC (eds) Cassava biology, production and utilization. CABI, Wallingford, pp 149–165

    Google Scholar 

  • Kapil RN (1961) Some embryological aspects of Euphorbia dulcis L. Phytomorphology 11:24–36

    Google Scholar 

  • Lai HG, Chen X, Li KM, Ou WJ, Ye JQ, Zhuang NS, Qin QL (2013) Meiotic observations of the microspore mother cells and unreduced diploidy pollen induction in Manihot esculenta. Chin J Trop Crops 34(5):821–828 (in Chinese with English abstract)

    Google Scholar 

  • Li YH, Kang XY, Wang SD, Zhang ZH, Chen HW (2008) Triploid induction in Populus alba × P. glandulosa by chromosome doubling of female gametes. Silvae Genet 57(1):37–40

    Google Scholar 

  • Mendiburu AO, Peloquin SJ (1977) The significance of 2n gametes in potato breeding. Theor Appl Genet 49:53–61

    Article  CAS  PubMed  Google Scholar 

  • Nassar NMA, Ortiz R (2007) Cassava improvement: challenges and impacts. J Agric Sci 145:163–171

    Article  Google Scholar 

  • Nassar HNMA, Nassar NMA, Vieira C, Saraiva IS (1995) Cytogenetic behaviour of interspecific hybrid of Manihot neusana Nassar and cassava, M. esculenta Crantz, and its backcross progeny. Can J Plant Sci 75:675–678

    Article  Google Scholar 

  • Ogburia MN, Yabuya T, Adachi T (2002) A cytogenetic study of bilateral sexual polyploidization in cassava (Manihot esculenta Crantz). Plant Breed 121:278–280

    Article  Google Scholar 

  • Otto SP, Whitton J (2000) Polyploid incidence and evolution. Annu Rev Genet 34(1):401–437

    Article  CAS  PubMed  Google Scholar 

  • Perera PIP, Quintero M, Dedicova B, Kularatne JDJS, Ceballos H (2013) Comparative morphology, biology and histology of reproductive development in three lines of Manihot esculenta Crantz (Euphorbiaceae: Crotonoideae). AoB Plants 5:pls046. doi:10.1093/aobpla/pls046

  • Qiu DB, Xie SW, Kong WM (1991) Megasporogenesis and development of embryo sac of Hevea brasiliensis. Chin J Trop Crops 12(1):1–9 (in Chinese with English abstract)

    Google Scholar 

  • Ramanna MS, Jacobsen E (2003) Relevance of sexual polyploidization for crop improvement—a review. Euphytica 133(1):3–18

    Article  Google Scholar 

  • Sreekumari MT, Jos JS, Nair SG (1999) ‘Sree Harsha’: a superior triploid hybrid in cassava. Euphytica 106:1–6

    Article  Google Scholar 

  • Tokuoka T, Tobe H (1995) Embryology and systematics of Euphorbiaceaesens. lat.: a review and perspective. J Plant Res 108(1):97–106

    Article  Google Scholar 

  • Wang YR, Deng GS, Li Y, Jiang JZ, Sun YH (2010) Progress in the mechanism of colchicine function on the tubulin and its cytological effects. Acta Bot Boreal Occident Sin 30(12):2570–2576 (in Chinese with English abstract)

    CAS  Google Scholar 

  • Wu H, Zheng S, He Y, Yan G, Bi Y, Zhu Y (2007) Diploid female gametes induced by colchicines in oriental lilies. Sci Hortic 114(1):50–53

    Article  CAS  Google Scholar 

  • Xiao YQ, Zheng SX, Long CL, Zheng L, Guan WL, Zhao Y (2007) Initial study on 2n-gametes induction of Strelitzia reginae. J Yunnan Agric Univ 22(4):475–479 (in Chinese with English abstract)

    CAS  Google Scholar 

  • Younis A, Hwang YJ, Lim KB (2014) Exploitation of induced 2n-gametes for plant breeding. Plant Cell Rep 33(2):215–223

    Article  CAS  PubMed  Google Scholar 

  • Zhang J, Guo JH, Chen XT, Wang Y, Wang WQ, Lai HG, Wu KX, Ni YY, Huang Q (2012) Technique for microscopical slides of cassava leaf for chromosome observation. Chin J Trop Crops 33(1):20–23 (in Chinese with English abstract)

    CAS  Google Scholar 

  • Zhong C, Zhang SN, Yu XH, Li Y, Hou XL, Li SJ (2010) Studies on the Induction of 2n gamete in Chinese cabbage and the production of tetraploid by sexual polyploidization. Acta Hortic Sin 37(11):1789–1795

    Google Scholar 

Download references

Acknowledgments

This work was financially supported by the National Nature Science Foundation of China (Grant No. 31360354), The National Basic Research Program (973 Program China (No. 2010CB126606), the Natural Science Foundation of Hainan Province (Grant No. 313041), the Hainan Province Major Science and Technology Project (ZDZX2013023) and The western plan and subject key areas construction project of Hainan university (ZXBJH-XK001).

Author information

Authors and Affiliations

  1. Hainan key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Agronomy, Hainan University, Haikou, 570228, Hainan, China

    Hang-Gui Lai, Xia Chen & Liu Jin-Ping

  2. College of Applied Science and Technology, Hainan University, Danzhou, 571737, China

    Zheng Chen

  3. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, Hainan, China

    Jian-Qiu Ye & Kai-Mian Li

Authors
  1. Hang-Gui Lai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xia Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zheng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jian-Qiu Ye
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kai-Mian Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Liu Jin-Ping
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Liu Jin-Ping.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lai, HG., Chen, X., Chen, Z. et al. Induction of female 2n gametes and creation of tetraploids through sexual hybridization in cassava (Manihot esculenta). Euphytica 201, 265–273 (2015). https://doi.org/10.1007/s10681-014-1207-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-014-1207-0

Keywords

Search

Navigation