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Meiotic chromosome pairing in intergeneric hybrids of colchicaceous ornamentals revealed by genomic in situ hybridization (GISH)

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Abstract

Diploid and triploid intergeneric hybrids obtained by crosses among Gloriosa superba ‘Lutea’ (2n = 2x = 22), G. ‘Marron Gold’ (2n = 4x = 44), Littonia modesta (2n = 2x = 22), and Sandersonia aurantiaca (2n = 2x = 24) were analyzed for their meiotic chromosome pairing in pollen mother cells by genomic in situ hybridization (GISH) with digoxigenin-labeled total DNA of one parent as probe. Chromosomes from each parent could be clearly distinguished in pollen mother cells of all the five intergeneric hybrids by GISH. For three diploid hybrids, L. modesta × G. superba ‘Lutea’ (2n = 2x = 22), L. modesta × S. aurantiaca (2n = 2x = 23) and S. aurantiaca × G. superba ‘Lutea’ (2n = 2x = 23), 0.04−0.27 autosyndetic bivalents (intragenomic pairing of non-homologous chromosomes) and 0.13−0.36 allosyndetic bivalents (intergenomic chromosome pairing) were observed per pollen mother cell, indicating that there are some homologous chromosomal regions within each genome and among the genomes of Gloriosa, Littonia and Sandersonia. Differences in the average number of allosyndetic bivalents per pollen mother cell among different genome combinations may reflect the evolutionary distances among the three genera, and Gloriosa and Littonia may be closely related to each other, while Sandersonia may have relatively distant relationships with Gloriosa and Littonia. For two triploid hybrids, L. modesta × G. ‘Marron Gold’ (2n = 3x = 33) and S. aurantiaca × G. ‘Marron Gold’ (2n = 3x = 34), no allosyndetic bivalents were observed. Based on the results obtained in the present study, possible utilization of the diploid and triploid intergeneric hybrids for further breeding of colchicaceous ornamentals is discussed.

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Acknowledgments

This work was supported in part by a Grant-in-Aid for Scientific Research (No. 20580023) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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  1. Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, Niigata, 950-2181, Japan

    Tomonari Kishimoto, Miki Yamakawa, Daisuke Nakazawa, Junji Amano, Sachiko Kuwayama & Masaru Nakano

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  1. Tomonari Kishimoto
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  2. Miki Yamakawa
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  3. Daisuke Nakazawa
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  4. Junji Amano
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  5. Sachiko Kuwayama
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  6. Masaru Nakano
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Correspondence to Masaru Nakano.

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Kishimoto, T., Yamakawa, M., Nakazawa, D. et al. Meiotic chromosome pairing in intergeneric hybrids of colchicaceous ornamentals revealed by genomic in situ hybridization (GISH). Euphytica 200, 251–257 (2014). https://doi.org/10.1007/s10681-014-1152-y

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  • DOI: https://doi.org/10.1007/s10681-014-1152-y

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