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Generation and validation of unique male sex-specific sequence tagged sites (STS) marker from diverse genotypes of dioecious Jojoba-Simmondsia chinensis (Link) Schneider

Abstract

DNA fingerprinting studies have been carried out with the physiologically mature male and female plants of Jojoba using 80 ISSR primers with a view to generate sex-linked markers. After bulk segregant analysis, two unique ISSR markers, viz. ISSR8481500 and VIS111317 have been developed which can be used for determining the sex at the seedling stage. Of the eighty primers tested on the pooled male DNA and pooled female DNA samples, six ISSR primers were found to be associated with sex expression. Of the six, only two primers ISSR848 and VIS11 generated unique male sex specific bands of ~1,500 and ~1,300 bp which were consecutively present in all the male genotypes and absent in all the respective female genotypes. The remaining four primers when tried on individuals of different genotypes were confined to their sex specificity in only two female genotypes and absent in their male counterparts. One of the male-sex specific markers, VIS111317 has also been cloned and sequenced which showed homology with a sex linked gene, DD44 from dioecious Silene species. Furthermore, VIS111317 was converted into a male sex-specific sequence tagged sites (STS) marker of 584 bp. The male specific STS marker thus developed has been verified and validated on 100 populations of male and female individuals from ten different genotypes of Jojoba to endorse the diagnostic reliability of the STS marker. This can gainfully be employed for screening of sex at seedling stage which would be quite helpful for uprooting the undesired plants, thereby, saving resources like labor, water, fertilizers and space for highly desirable female plants.

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Acknowledgments

The authors are grateful to the Department of Science and Technology, Delhi, Government of India for the sanction of a Major research project (SERB/SR/SO/PS/05/2012) to Veena Agrawal and DST young scientist project [SR/FT/LS-109/2008 (G)] to Kuldeep Sharma. Monika Heikrujam is indebted to CSIR, India for the award of CSIR JRF and SRF. We are also thankful to Mr. LR Saini, AJORP, Rajsthan for providing plant materials.

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Correspondence to Veena Agrawal.

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Heikrujam, M., Sharma, K., Kumar, J. et al. Generation and validation of unique male sex-specific sequence tagged sites (STS) marker from diverse genotypes of dioecious Jojoba-Simmondsia chinensis (Link) Schneider. Euphytica 199, 363–372 (2014). https://doi.org/10.1007/s10681-014-1136-y

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  • DOI: https://doi.org/10.1007/s10681-014-1136-y

Keywords

  • Dioecious
  • Inter simple sequence repeats (ISSRs)
  • Sex specific marker
  • Simmondsia chinensis
  • Sequence tagged sites