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Differentially expressed genes related to the formation of russet fruit skin in a mutant of ‘Dangshansuli’ pear (Pyrus bretchnederi Rehd.) determined by suppression subtractive hybridization

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Abstract

The mature fruit skin of ‘Dangshansuli’ pear (Pyrus bretchnederi Rehd.) is yellow-green, but the fruit of the mutant, ‘Xiusu’, is russetted. To understand the mechanism of russet formation, the suppression subtractive hybridization libraries were used to screen for differentially expressed genes between the wild type pear and the mutant. Three hundred and twenty nine and 366 unigenes were obtained from 934 and 929 colonies in the forward and reverse libraries, respectively. The results indicated that phenylpropanoid metabolism, ethylene metabolism and secondary metabolism might be involved in the russet formation process, and fruit skin is likely to be regulated by lignin synthesis, polyamine and H2O2 signalling. Furthermore, the accumulation of lignin and H2O2, together with up-regulated POD enzyme genes expression in the exocarp of ‘Xiusu’ compare with ‘Dangshansuli’ pear control, provided a comprehensive transcriptomics view on the coordination of the russet formation of mutant pear.

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Abbreviations

bHLH:

Basic helix-loop-helix

CCoAOMT:

Caffeoyl coenzyme A O-methyltransferase

COMT:

Caffeic acid O-methyltransferase

DAFB:

Day after full bloom

EG:

Endo-1,4-beta-d-glucanase

ISSR:

Inter-simple sequence repeat

PCR:

Polymerase chain reaction

RAPD:

Randomly amplified polymorphic DNA

SAMDC:

S-adenosylmethionine decarboxylase

SAMS:

S-adenosyl-l-methionine synthetase

SSH:

Suppression subtractive hybridization

TEM:

Transmission electron microscopy

XTH:

Xyloglucan endotransglucosylase/hydrolase

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Acknowledgments

This project was supported by the National Natural Science Foundation of China (31101519), the earmarked fund for China Agriculture Research System (CARS-29-14), and the Foundation for Young Talents in universities of Anhui Province (2012SQRL060ZD). The authors thank Prof. Christopher Brian Watkins (Cornell University, NY, USA) for helpful suggestions and paper revises.

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Authors and Affiliations

  1. Key Laboratory of Pomology, Anhui Agricultural University, Hefei, Anhui, People’s Republic of China

    Wei Heng, Li Liu, Meng-dong Wang, Bing Jia, Pu Liu, Zhen-feng Ye & Li-wu Zhu

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  1. Wei Heng
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  2. Li Liu
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  3. Meng-dong Wang
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  4. Bing Jia
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  5. Pu Liu
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  6. Zhen-feng Ye
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  7. Li-wu Zhu
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Correspondence to Li-wu Zhu.

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Wei Heng and Li Liu should be regarded as joint first authors.

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Heng, W., Liu, L., Wang, Md. et al. Differentially expressed genes related to the formation of russet fruit skin in a mutant of ‘Dangshansuli’ pear (Pyrus bretchnederi Rehd.) determined by suppression subtractive hybridization. Euphytica 196, 285–297 (2014). https://doi.org/10.1007/s10681-013-1032-x

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  • DOI: https://doi.org/10.1007/s10681-013-1032-x

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