Abstract
Organic acids play a primary role in defining grape flavor and wine organoleptic properties. China-originated grape species contain high level acid, which limits to a certain extent, the marketing of fruits and their processing products. The objective of this study is to compare the changes of four organic acids along with grape berry development in cultivars native to China and Europe as well as some hybrids, and to preliminarily ascertain the reason for the high acid concentration in Chinese species. The results indicate that although the variation of titratable acidity during berry development strongly correlates with malic acid, the most significant difference amongst species at harvest lay in the concentration of tartaric acid. The Chinese-type species, V. quinquangularis, V. davidi and V. amurensis, contained higher levels of tartaric acid than European species. But this difference could be narrowed by interspecific hybridization. L-IdnDH, a key enzyme involved in tartaric acid synthesis, presented high homology in amino acid sequence for these species. Grape species with high level of tartaric acid did not have high transcript abundance of L-IdnDH (XM_002267626.2 and NM_001280954.1), but showed a slower decline in L-IdnDH amount during berry development.
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References
Amerine M, Berg H, Kunkee R, Ough C, Singleton V (1982) The composition of grapes. In: Webb AD (ed) The technology of wine making. AVI, Westport, pp 77–139
Boso Alonso S, Kassemeyer HH (2008) Different susceptibility of European grapevine cultivars for downy mildew. Vitis 47(1):39–49
Boulton R, Singleton V, Bisson L, Kunkee R (1995) selection of state of ripeness for harvest and harvesting. In: Boulton R (ed) Principles and practices of wihemaking. International Thomson, Champman & Hall, pp 52–60
DeBolt S, Cook DR, Ford CM (2006) l-Tartaric acid synthesis from vitamin C in higher plants. Proc Natl Acad Sci 103(14):5608–5613
DeBolt S, Melino V, Ford CM (2007) Ascorbate as a biosynthetic precursor in plants. Ann Bot 99(1):3–8
Fuleki T, Pelavo E, Palabay R (1993) Carboxylic acid composition of authentic varietal and commercial grape juices. J AOAC Int 76(3):591–600
García Romero E, Sánchez Muñoz G, Martín Alvarez P, Cabezudo Ibáñez M (1993) Determination of organic acids in grape musts, wines and vinegars by high-performance liquid chromatography. J Chromatogr A 655(1):111–117
Iland P, Coombe B (1988) Malate, tartrate, potassium, and sodium in flesh and skin of Shiraz grapes during ripening: concentration and compartmentation. Am J Enol Viticult 39(1):71–76
Lamikanra O, Inyang ID, Leong S (1995) Distribution and effect of grape maturity on organic acid content of red muscadine grapes. J Agric Food Chem 43(12):3026–3028
Li D, Wan Y, Wang Y, He P (2008) Relatedness of resistance to anthracnose and to white rot in Chinese wild grapes. Vitis 47(4):213–215
Liang Z, Wu B, Fan P, Yang C, Duan W, Zheng X, Liu C, Li S (2008) Anthocyanin composition and content in grape berry skin in Vitis germplasm. Food Chem 111(4):837–844
Liang Z, Sang M, Ma A, Zhao S, Zhong G-y, Li S (2011) Inheritance of sugar and acid contents in the ripe berries of a tetraploid × diploid grape cross population. Euphytica 182(2):251–259
Liu HF, Wu BH, Fan PG, Xu HY, Li SH (2007) Inheritance of sugars and acids in berries of grape (Vitis vinifera L.). Euphytica 153(1–2):99–107
Ma Y, Zhang Y, Shao H, Lu J (2010) Differential physio-biochemical responses to cold stress of cold-tolerant and non-tolerant grapes (Vitis L.) from China. J Agron Crop Sci 196(3):212–219
Malipiero U, Ruffner H, Rast D (1987) Ascorbic to tartaric acid conversion in grapevines. J Plant Physiol 129(1):33–40
Melino V, Soole K, Ford C (2009) Ascorbate metabolism and the developmental demand for tartaric and oxalic acids in ripening grape berries. BMC Plant Biol 9(1):145
Muñoz-Robredo P, Robledo P, Manríquez D, Molina R, Defilippi BG (2011) Characterization of sugars and organic acids in commercial varieties of table grapes. Chil J Agric Res 71(3):452–458
Ruffner HP (1982) Metabolism of tartaric and malic acids in Vitis: a review-part B. Vitis 21:346–358
Ruffner HP, Hawker JS (1977) Control of glycolysis in ripening berries of Vitis vinifera. Phytochemistry 16(8):1171–1175
Ruffner HP, Kliewer WM (1975) Phosphoenolpyruvate carboxykinase activity in grape berries. Plant Physiol 56(1):67–71
Ruffner HP, Hawker JS, Hale CR (1976) Temperature and enzymic control of malate metabolism in berries of Vitis vinifera. Phytochemistry 15(12):1877–1880
Sabir A, Kafkas E, Tangolar S (2010) Distribution of major sugars, acids, and total phenols in juice of five grapevine (Vitis spp.) cultivars at different stages of berry development. Span J Agric Res 8(2):425–433
Saito K, Morita SI, Kasai Z (1984) Synthesis of l-dextro-tartaric acid from 5-keto-d-gluconic acid in Pelargonium. Plant Cell Physiol 25:1223–1232
Shiraishi M (1995) Proposed descriptors for organic acids to evaluate grape germplasm. Euphytica 81(1):13–20
Soyer Y, Koca N, Karadeniz F (2003) Organic acid profile of Turkish white grapes and grape juices. J Food Compost Anal 16(5):629–636
Staudt G, Kassemeyer H (1995) Evaluation of downy mildew resistance in various accessions of wild Vitis species. Vitis 34(4):225–228
Sweetman C, Deluc LG, Cramer GR, Ford CM, Soole KL (2009) Regulation of malate metabolism in grape berry and other developing fruits. Phytochemistry 70(11–12):1329–1344
Sweetman C, Wong DC, Ford CM, Drew DP (2012) Transcriptome analysis at four developmental stages of grape berry (Vitis vinifera cv. Shiraz) provides insights into regulated and coordinated gene expression. BMC Genomics 13(1):691
Volschenk H, Van Vuuren H H, Viljoen-Bloom M (2006) Malic acid in wine: origin, function and metabolism during vinification. S Afr J Enol Vitic 27(2):123
Wan Y, Schwaninger H, He P, Wang Y (2007) Comparison of resistance to powdery mildew and downy mildew in Chinese wild grapes. Vitis 46(3):132
Wang Y, Liu Y, He P, Chen J, Lamikanra O, Lu J (1995) Evaluation of foliar resistance to Uncinula necator in Chinese wild Vitis species. Vitis 34(3):159–164
Wen YQ, Li JM, Zhang ZZ, Zhang YF, Pan QH (2010) Antibody preparation, gene expression and subcellular localization of l-Idonate dehydrogenase in grape berry. Biosci Biotechnol Biochem 74(12):2413–2417
Winkler AJ, Cook JA, Kliewer WM, Lider LA (1962) General Viticulture. Berkeley and Los Angeles, California
Xu C, Zhang Y, Cao L, Lu J (2010) Phenolic compounds and antioxidant properties of different grape cultivars grown in China. Food Chem 119(4):1557–1565
Xu K, Wang A, Brown S (2012) Genetic characterization of the Ma locus with pH and titratable acidity in apple. Mol Breed 30(2):899–912
Zhu L, Zhang Y, Lu J (2012) Phenolic contents and compositions in skins of red wine grape cultivars among various genetic backgrounds and originations. Int J Mol Sci 13(3):3492–3510
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This work was financially supported by the Beijing Municipal Natural Science Foundation (No. 6092014 to Pan QH).
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Ya-Qin Wen and Jing Cui equally contributed to this work.
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Wen, YQ., Cui, J., Zhang, Y. et al. Comparison of organic acid levels and L-IdnDH expression in Chinese-type and European-type grapes. Euphytica 196, 63–76 (2014). https://doi.org/10.1007/s10681-013-1014-z
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DOI: https://doi.org/10.1007/s10681-013-1014-z