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Genetic analysis of seed mineral accumulation affected by phosphorus deprivation in Brassica napus

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Abstract

The mineral content of plant seeds depends on both environmental and genetic factors. The aim of this study was to detect quantitative trait loci (QTLs), and their candidate genes, for the accumulation of phosphorus (P), calcium (Ca), magnesium (Mg), zinc (Zn), copper (Cu), iron (Fe), and manganese (Mn) in seeds of Brassica napus under normal and low P conditions using an F 10 recombinant inbred line (RIL) population. Two-year field trials were conducted to investigate seed mineral accumulation. The results showed a significant decrease in most of the minerals in the BE RIL population, as well as in two parental lines, when grown in a low P environment compared to a normal P environment. In total, 60 putative QTLs were identified, 33 of which overlapped with each other in nine genomic regions in seven linkage groups. Twenty-one of the 60 significant QTLs co-located with eight seed weight QTLs, and only five overlapped with three seed yield QTLs. Moreover, only six QTLs for the same minerals were identified both in normal and low P levels. By comparative mapping of Arabidopsis and B. napus, 148 orthologs of 97 genes involved in the homeostasis of the seven minerals in Arabidopsis were associated with 47 QTLs corresponding to 24 chromosomal regions. These results offer insight into the genetic basis of mineral accumulation across different P conditions in seeds of B. napus and allow the potential utilization of QTLs in biofortification.

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References

  • Aastveit AH, Aastveit K (1993) Effects of genotype-environment interactions on genetic correlations. Theor Appl Genet 86:1007–1013

    Article  Google Scholar 

  • Blair M, Astudillo C, Grusak M, Graham R, Beebe S (2009) Inheritance of seed iron and zinc concentrations in common bean (Phaseolus vulgaris L.). Mol Breed 23:197–207

    Article  CAS  Google Scholar 

  • Brennan RF, Longnecker NE (2001) Effects of the concentration of manganese in the seed in alleviating manganese deficiency of Lupinus angustifolius L. Aust J Exp Agric 41:1199–1205

    Article  CAS  Google Scholar 

  • Broadley MR, White PJ, Hammond JP, Zelko I, Lux A (2007) Zinc in plants. New Phytol 173:677–702

    Article  PubMed  CAS  Google Scholar 

  • Cichy KA, Caldas GV, Snapp SS, Blair MW (2009) QTL analysis of seed iron, zinc, and phosphorus levels in an Andean bean population. Crop Sci 49:1742–1750

    Article  CAS  Google Scholar 

  • Conn SJ, Berninger P, Broadley MR, Gilliham M (2012) Exploiting natural variation to uncover candidate genes that control element accumulation in Arabidopsis thaliana. New Phytol 193:859–866

    Article  PubMed  CAS  Google Scholar 

  • Cordell D, Drangert JO, White S (2009) The story of phosphorus: global food security and food for thought. Glob Environ Change 19:292–305

    Article  Google Scholar 

  • Ding GD, Yang M, Hu YF, Liao Y, Shi L, Xu FS, Meng JL (2010) Quantitative trait loci affecting seed mineral concentrations in Brassica napus grown with contrasting phosphorus supplies. Ann Bot 105:1221–1234

    Article  PubMed  CAS  Google Scholar 

  • Ding G, Zhao Z, Liao Y, Hu Y, Shi L, Long Y, Xu F (2012) Quantitative trait loci for seed yield and yield-related traits, and their responses to reduced phosphorus supply in Brassica napus. Ann Bot 109:747–759

    Article  PubMed  CAS  Google Scholar 

  • Doerge RW, Churchill GA (1996) Permutation tests for multiple loci affecting a quantitative character. Genetics 142:285–294

    PubMed  CAS  Google Scholar 

  • Duan HY, Shi L, Ye XS, Wang YH, Xu FS (2009) Identification of phosphorous efficient germplasm in oilseed rape. J Plant Nutr 32:1148–1163

    Article  CAS  Google Scholar 

  • Garcia-Oliveira AL, Tan L, Fu Y, Sun C (2009) Genetic identification of quantitative trait loci for contents of mineral nutrients in rice grain. J Integr Plant Biol 51:84–92

    Article  PubMed  CAS  Google Scholar 

  • Guerinot ML (2000) The ZIP family of metal transporters. Biochim Biophys Acta Biomembr 1465:190–198

    Article  CAS  Google Scholar 

  • Higuchi K, Suzuki K, Nakanishi H, Yamaguchi H, Nishizawa NK, Mori S (1999) Cloning of nicotianamine synthase, novel genes involved in the biosynthesis of phytosiderophores. Plant Physiol 119:471–480

    Article  PubMed  CAS  Google Scholar 

  • House W (1999) Trace element bioavailability as exemplified by iron and zinc. Field Crop Res 60:115–141

    Article  Google Scholar 

  • Klein MA, Grusak MA (2009) Identification of nutrient and physical seed trait QTL in the model legume Lotus japonicus. Genome 52:677–691

    Article  PubMed  CAS  Google Scholar 

  • Kochian LV (2012) Plant nutrition: rooting for more phosphorus. Nature 488:466–467

    Article  PubMed  CAS  Google Scholar 

  • Lonergan PF, Pallotta MA, Lorimer M, Paull JG, Barker SJ, Graham RD (2009) Multiple genetic loci for zinc uptake and distribution in barley (Hordeum vulgare). New Phytol 184(1):168–179

    Article  PubMed  CAS  Google Scholar 

  • Long Y, Shi JQ, Qiu D, Li RY, Zhang CY, Wang J, Hou JN, Zhao JW, Shi L, Park BS, Choi SR, Lim YP, Meng JL (2007) Flowering time quantitative trait Loci analysis of oilseed brassica in multiple environments and genomewide alignment with Arabidopsis. Genetics 177:2433–2444

    PubMed  CAS  Google Scholar 

  • Lung’aho MG, Mwaniki AM, Szalma SJ, Hart JJ, Rutzke MA, Kochian LV, Glahn RP, Hoekenga OA (2011) Genetic and physiological analysis of iron biofortification in Maize Kernels. PLoS ONE 6(6):e20429

    Article  PubMed  Google Scholar 

  • Lynch JP (2011) Root phenes for enhanced soil exploration and phosphorus acquisition: tools for future crops. Plant Physiol 156:1041–1049

    Article  PubMed  CAS  Google Scholar 

  • Malhi SS (2009) Effectiveness of seed-soaked Cu, autumn-versus spring-applied Cu, and Cu-treated P fertilizer on seed yield of wheat and residual nitrate-N for a Cu-deficient soil. Can J Plant Sci 89:1017–1030

    Article  CAS  Google Scholar 

  • Norton GJ, Deacon CM, Xiong LZ, Huang SY, Meharg AA, Price AH (2010) Genetic mapping of the rice ionome in leaves and grain: identification of QTLs for 17 elements including arsenic, cadmium, iron and selenium. Plant Soil 329:139–153

    Article  CAS  Google Scholar 

  • Peleg Z, Cakmak I, Ozturk L, Yazici A, Jun Y, Budak H, Korol AB, Fahima T, Saranga Y (2009) Quantitative trait loci conferring grain mineral nutrient concentrations in durum wheat × wild emmer wheat RIL population. Theor Appl Genet 119:353–369

    Article  PubMed  CAS  Google Scholar 

  • Raboy V (1997) Accumulation and storage of phosphate and minerals. In: Larkins BA, Vasil IK (eds) Cellular and molecular biology of plant seed development. Kluwer, Dordrecht, pp 441–477

    Chapter  Google Scholar 

  • Raboy V, Young KA, Dorsch JA, Cook A (2001) Genetics and breeding of seed phosphorus and phytic acid. J Plant Physiol 158:489–497

    Article  CAS  Google Scholar 

  • Raghothama KG (1999) Phosphate acquisition. Annu Rev Plant Physiol Plant Mol Biol 50:665–693

    Article  PubMed  CAS  Google Scholar 

  • Sankaran R, Huguet T, Grusak M (2009) Identification of QTL affecting seed mineral concentrations and content in the model legume Medicago truncatula. Theor Appl Genet 119:241–253

    Article  PubMed  CAS  Google Scholar 

  • Schachtman DP, Shin R (2007) Nutrient sensing and signaling: NPKS. Annu Rev Plant Biol 58:47–69

    Article  PubMed  CAS  Google Scholar 

  • Schranz ME, Lysak MA, Mitchell-Olds T (2006) The ABC’s of comparative genomics in the Brassicaceae: building blocks of crucifer genomes. Trends Plant Sci 11:535–542

    Google Scholar 

  • Shi RL, Li HW, Tong YP, Jing RL, Zhang FS, Zou CQ (2008) Identification of quantitative trait locus of zinc and phosphorus density in wheat (Triticum aestivum L.) grain. Plant Soil 306:95–104

    Article  CAS  Google Scholar 

  • Simić D, Mladenović Drinić S, Zdunić Z, Jambrović A, Ledencan T, Brkić J, Brkić A, Brkić I (2012) Quantitative trait loci for biofortification traits in maize grain. J Hered 103(1):47–54

    Article  PubMed  Google Scholar 

  • Vance CP, Uhde-Stone C, Allan DL (2003) Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. New Phytol 157:423–447

    Article  CAS  Google Scholar 

  • Vreugdenhil D, Aarts MGM, Koornneef M, Nelissen H, Ernst WHO (2004) Natural variation and QTL analysis for cationic mineral content in seeds of Arabidopsis thaliana. Plant Cell Environ 27:828–839

    Article  CAS  Google Scholar 

  • Wang HZ (2010) Review and future development of rapeseed industry in China. Chin J Oil Crop Sci 32(2):300–302

    Google Scholar 

  • Wang SC, Bastern J, Zeng ZB (2011) Windows QTL cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh

    Google Scholar 

  • Waters BM, Grusak MA (2008) Quantitative trait locus mapping for seed mineral concentrations in two Arabidopsis thaliana recombinant inbred populations. New Phytol 179:1033–1047

    Article  PubMed  CAS  Google Scholar 

  • Waters BM, Sankaran RP (2011) Moving micronutrients from the soil to the seeds: genes and physiological processes from a biofortification perspective. Plant Sci 180:562–574

    Article  PubMed  CAS  Google Scholar 

  • White PJ, Broadley MR (2009) Biofortification of crops with seven mineral elements often lacking in human diets—iron, zinc, copper, calcium, magnesium, selenium and iodine. New Phytol 182:49–84

    Article  PubMed  CAS  Google Scholar 

  • White PJ, Brown PH (2010) Plant nutrition for sustainable development and global health. Ann Bot 105:1073–1080

    Article  PubMed  CAS  Google Scholar 

  • White PJ, Veneklaas EJ (2012) Nature and nurture: the importance of seed phosphorus content. Plant Soil 357:1–8

    Article  CAS  Google Scholar 

  • Yan X, Wu P, Ling H, Xu G, Xu F, Zhang Q (2006) Plant nutriomics in China: an overview. Ann Bot 98:473–482

    Article  PubMed  CAS  Google Scholar 

  • Yang M, Ding GD, Shi L, Feng J, Xu FS, Meng JL (2010) Quantitative trait loci for root morphology in response to low phosphorus stress in Brassica napus. Theor Appl Genet 121:181–193

    Article  PubMed  CAS  Google Scholar 

  • Zeng ZB (1994) Precision mapping of quantitative trait loci. Genetics 136:1457–1468

    PubMed  CAS  Google Scholar 

  • Zhang D, Cheng H, Geng LY, Kan GZ, Cui SY, Meng QC, Gai JY, Yu DY (2009) Detection of quantitative trait loci for phosphorus deficiency tolerance at soybean seedling stage. Euphytica 167:313–322

    Google Scholar 

  • Zhang X, Zhang G, Guo L, Wang H, Zeng D, Dong G, Qian Q, Xue D (2011) Identification of quantitative trait loci for Cd and Zn concentrations of brown rice grown in Cd-polluted soils. Euphytica 180:173–179

    Article  CAS  Google Scholar 

  • Zhao J, Jamar DC, Lou P, Wang Y, Wu J, Wang X, Bonnema G, Koornneef M, Vreugdenhil D (2008) Quantitative trait loci analysis of phytate and phosphate concentrations in seeds and leaves of Brassica rapa. Plant Cell Environ 31:887–900

    Article  PubMed  CAS  Google Scholar 

  • Zhu YG, Smith SE (2001) Seed phosphorus (P) content affects growth, and P uptake of wheat plants and their association with arbuscular mycorrhizal (AM) fungi. Plant Soil 231:105–112

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Basic Research and Development Program [2011CB100301], the National Natural Science Foundation [31201672], China and the Specialized Research Found for the Doctoral Program of Higher Education, Ministry of Education of China [4010-121045]. This work was also partly supported by the open funds of the National Key Laboratory of Crop Genetic Improvement, Wuhan, China.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, China

    Guangda Ding, Lei Shi, Hua Zhao, Kede Liu & Fangsen Xu

  2. Microelement Research Centre, Huazhong Agricultural University, Wuhan, 430070, China

    Guangda Ding, Lei Shi, Hua Zhao, Hongmei Cai & Fangsen Xu

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  1. Guangda Ding
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  2. Lei Shi
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  3. Hua Zhao
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  4. Hongmei Cai
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  5. Kede Liu
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  6. Fangsen Xu
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Correspondence to Fangsen Xu.

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Ding, G., Shi, L., Zhao, H. et al. Genetic analysis of seed mineral accumulation affected by phosphorus deprivation in Brassica napus . Euphytica 193, 251–264 (2013). https://doi.org/10.1007/s10681-013-0933-z

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