Skip to main content
SpringerLink
Log in

Inheritance and molecular tagging of MYMIV resistance gene in blackgram (Vigna mungo L. Hepper)

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

Yellow Mosaic disease (YMD) is one of the most destructive diseases of blackgram (Vigna mungo) causing heavy yield losses every year. Mungbean Yellow Mosaic India Virus (MYMIV) is one of the YMD causing begomoviruses prevalent in the major blackgram growing area (northern and central part) of India. Inheritance of MYMIV resistance gene was studied in blackgram using F1, F2 and F2:3 derived from cross DPU 88-31(resistant)× AKU 9904 (susceptible). The results of genetic analysis showed that a single dominant gene controls the MYMIV resistance in blackgram genotype DPU 88-31. The F2 population from the same cross was also used to tag and map the MYMIV resistance gene using SSR markers. Out of 361 markers, 31 were found polymorphic between the parents. However, marker CEDG 180 was found to be linked with resistance gene following the bulked segregant analysis. This marker was mapped in the F2 mapping population of 168 individuals at a map distance of 12.9 cm. The validation of this marker in nine resistant and seven susceptible genotypes has suggested its use in marker assisted breeding for developing MYMIV resistant genotypes in blackgram.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Anjum Tuba K, Gupta S, Datta S (2010) Mapping of MYMIV and powdery mildew resistant gene in blackgram. Electron J Plant Breed 1:1148–1152

    Google Scholar 

  • Basak J, Kundagrami S, Ghose TK, Pal A (2004) Development of yellow mosaic virus (YMV) resistance linked DNA marker in Vigna mungo from populations segregating for YMV reaction. Mol Breed 14:375–383

    Article  CAS  Google Scholar 

  • Biswas KK, Varma A (2001) Agroinoculation: a method of screening germplasm resistance to mungbean yellow mosaic geminivirus. Indian Phytopathol 54:134–141

    Google Scholar 

  • Blair MW, Pedraza F, Buendia HF, Gaitan-Solis E, Beebe SE, Gepts P, Tohme J (2003) Development of a genome-wide anchored microsatellite map for common bea (Phaseolus vulgaris L.). Theor Appl Genet 107:1362–1374

    Article  PubMed  CAS  Google Scholar 

  • Buhariwalla HK, Jayashree B, Eshwar K, Crouch JW (2005) Development of ESTs from chickpea roots and their use in diversity analysis of the Cicer species. BMC Plant Bio 5:16–29

    Article  Google Scholar 

  • Chatieng B, Kaga A, Tomooka N, Isemura J, Kurocla Y, Vaughan DA (2006) Development of a blackgram [Vigna mungo (L.) Hepper] linkage map and its comparison with an azukibean [Vigna angularis (Willd.) Ohwi & Ohashi]. Theor Appl Genet 113:1261–1269

    Article  Google Scholar 

  • Dahiya BS, Singh K, Brar JS (1977) Incorporation of resistance to mungbean yellow mosaic virus in blackgram (Vigna mungo L). Trop Grain Legume Bull 9:28–32

    Google Scholar 

  • Datta S, Kaashyap M, Kumar S (2010) Amplification of chickpea-specific SSR primers in Cajanus species and their validity in diversity analysis. Plant Breed 129:334–340

    Article  CAS  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Dwivedi S, Singh DP (1985) Inheritance of resistance to yellow mosaic virus in a wide cross of blackgram (Vigna mungo L). Z Pflanzenzuecht 95:281–284

    Google Scholar 

  • Fauquet CM, Bisaro DM, Briddon RW, Brown JK, Harrison BD, Rybicki EP, Stenger DC, Stanley J (2003) Revision of taxonomic criteria for species demarcation in the family Geminiviridae, an updated list of begomovirus species. Arch Virol 148:405–421

    Article  PubMed  CAS  Google Scholar 

  • Gaitan-Solis E, Duque MC, Edwards KJ, Tohme J (2002) Microsatellite repeats in common bean (Phaseolus vulgaris): isolation, characterization and cross-species amplification in Phaseolus ssp. Crop Sci 42:2128–2136

    Article  CAS  Google Scholar 

  • Gao Z, Eyers S, Thomas C, Ellis N, Maule A (2004) Identification of markers tightly linked to sbm recessive genes for resistance to pea seed-borne mosaic virus. Theor Appl Genet 109:488–494

    PubMed  CAS  Google Scholar 

  • Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research, 2nd edn. Wiley, New York, p 680

    Google Scholar 

  • Gupta S, Kumar S, Singh RA, Chandra S (2005) Identification of a single dominant gene for resistance to mungbean yellow mosaic virus in blackgram. SABRAO J Breed Genet 37:85–89

    Google Scholar 

  • Gupta SK, Souframanien J, Gopalakrishna T (2008) Construction of a genetic linkage map of blackgram based on molecular markers and comparative studies. Genome 51:628–637

    Article  PubMed  CAS  Google Scholar 

  • Gupta S, Gupta DS, Anjum TK, Pratap A, Kumar J (2013) Transferability of simple sequence repeat markers in blackgram. Aust J Agric Sci (in press)

    Google Scholar 

  • Han OK, Kaga A, Isemusa T, Wang XW, Tomooka N, Vaughan DA (2005) A genetic linkage map for azukibean [Vigna angularis (Willd.) Ohwi & Ohashi]. Theor Appl Genet 111:1278–1287

    Article  PubMed  CAS  Google Scholar 

  • Haq QMI, Rouhibakhsh A, Ali A, Malathi VG (2011) Infectivity analysis of blackgram isolate of MYMV and genetic assortment with MYMIV in selected host. Virus Genes 42:429–439

    Article  PubMed  CAS  Google Scholar 

  • Jacob SS, Vanitharani R, Karthikeyan AS, Chinchore Y, Thilaichidambaram P, Veluthambi K (2003) MYMV Vi agroinfection by codelivering of DNA A and DNA B from one Agrobacterium strain. Plant Dis 87:247–251

    Article  CAS  Google Scholar 

  • Jeong SC, Saghai Maroof MA (2004) Detection and genotyping of SNPs tightly linked to two disease resistance loci, Rsv1 and Rsv3, of soybean. Plant Breed 123:305–310

    Article  CAS  Google Scholar 

  • Kaushal RP, Singh BM (1988) Inheritance of disease resistance in blackgram (Vigna mungo) to MYMV. Indian J Agric Sci 58:123–124

    Google Scholar 

  • Kumar SV, Tan SG, Quah SC, Yusoff K (2002a) Isolation of microsatellite in mungbean, Vigna radiata. Mol Eco Notes 2:96–98

    Article  CAS  Google Scholar 

  • Kumar SV, Tan SG, Quah SC, Yusoff K (2002b) Isolation of microsatellite markers in mungbean, Vigna radiata. Mol Eco Notes 2:293–295

    Article  CAS  Google Scholar 

  • Kumar J, Choudhary AK, Solanki RK, Pratap A (2011a) Towards marker-assisted selection in pulses: a review. Plant Breed 130:297–313

    Article  CAS  Google Scholar 

  • Kumar J, Pratap A, Solanki RK, Gupta DS, Goyal A, Chaturvedi SK, Nadarajan N, Kumar S (2011b) Genomic resources for improving food legume crops. J Agric Sci 150:289–318

    Article  Google Scholar 

  • Li DC, Fatokun CA, Ubi B, Singh BB, Scoles GJ (2001) Determining genetic similarity and relationships among cowpea breeding lines and cultivars by microsatellite markers. Crop Sci 41:89–197

    Google Scholar 

  • Maiti S, Basak J, Kundagrami S, Kundu A, Pal A (2011) Molecular marker-assisted genotyping of mungbean yellow mosaic India virus resistant germplasms of mungbean and urdbean. Mol Biotech 47:95–104

    Article  CAS  Google Scholar 

  • Malathi VG, John P (2008) Gemini viruses infecting legumes. In: Rao GP, Lava Kumar P, Holguin-Pena RJ (eds) Characterization, diagnosis and management of plant viruses. Studium Press, Houston, pp 97–123

    Google Scholar 

  • Mandal B, Varma A, Malathi VG (1997) Systemic infection of V. mungo using the cloned DNAs of the blackgram isolate of mungbean yellow mosaic geminivirus through agroinoculation and transmission of the progeny virus by whiteflies. J Phytopathol 145:505–510

    Article  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  Google Scholar 

  • Naimuddin AM, Pratap A (2011) First report of natural infection of mungbean yellow mosaic India virus in two wild species of Vigna. New Disease Rep 23:21

    Article  Google Scholar 

  • Pal SS, Dhaliwal HS, Bains SS (1991) Inheritance of resistance to yellow mosaic virus in some Vigna species. Plant Breed 106:168–171

    Article  Google Scholar 

  • Reddy KR, Singh DP (1995) Inheritance of resistance to mungbean yellow mosaic virus. Madras Agric J 88:199–201

    Google Scholar 

  • Shoba D, Manivannan N, Vindhiyavarman P, Nigam SN (2012) SSR markers associated for late leaf spot disease resistance by bulked segregant analysis in groundnut (Arachis hypogaea L). Euphytica. doi:10.1007/s10681-012-0718-9

    Google Scholar 

  • Singh DP (1980) Inheritance of resistance to yellow mosaic virus in blackgram (Vigna mungo L.). Theor Appl Genet 52:233–235

    Google Scholar 

  • Singh RA, Gurha SN (1994) Influence of cropping season on the incidence of yellow mosaic virus in mungbean genotypes. Indian J Pulses Res 7:206–208

    Google Scholar 

  • Somta P, Seehalak W, Srinives P (2009) Development, characterization and cross-species amplification of mungbean (Vigna radiata) genic microsatellite markers. Conserv Genet doi. doi:10.1007/s10592-009-9860-x

    Google Scholar 

  • Souframanien J, Gopalakrishna T (2006) ISSR and SCAR markers linked to the mungbean yellow mosaic virus (MYMV) resistance gene in blackgram [Vign amungo (L.) Hepper]. Plant Breed 125:619–622

    Article  CAS  Google Scholar 

  • Stevens WL (1939) Solution to a geometrical problem in probability. Ann Eugen 9:315–320

    Article  Google Scholar 

  • Urrea CA (1996) A codominant randomly amplified polymorphic DNA (RAPD) marker useful for indirect selection of bean golden mosaic virus resistance in common bean. J Am Soc Hortic Sci 12:1035–1039

    Google Scholar 

  • Varma A, Malathi G (2003) Emerging geminivirus problems: a serious threat to crop production. Ann Appl Biol 142:145–164

    Article  CAS  Google Scholar 

  • Verma RPS, Singh DP (1986) The allelic relationship of genes giving resistance to mungbean yellow mosaic virus in blackgram. Theor Appl Genet 72:737–738

    Article  Google Scholar 

  • Wang XW, Kaga A, Tomooka N, Vaughan DA (2004) The development of SSR markers by a new method in plants and their application to gene flow studies in azuki bean [Vigna angularis (Willd.) Ohwi & Ohashi]. Theor Appl Genet 109:352–360

    PubMed  CAS  Google Scholar 

  • Yu YG, Saghai Maroof MA, Buss GR (1996) Divergence and allelomorphic relationship of a soybean virus resistance gene based on tightly linked DNA microsatellite and RFLP markers. Theor Appl Genet 92:64–69

    Article  CAS  Google Scholar 

  • Zhang MC, Wang DM, Zheng Z, Humphry M, Liu CJ (2008) Development of PCR based markers for a major locus conferring powdery mildew resistance in mungbean (Vigna radiata). Plant Breed 127:429–432

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by research grant from Department of Biotechnology, Government of India. The authors thank Director, IIPR and Head, Crop Improvement Division, IIPR for providing necessary facilities required to carry out this study.

Author information

Authors and Affiliations

  1. AICRP on MULLaRP Unit, Indian Institute of Pulses Research (Indian Council of Agricultural Research), Kanpur, Uttar Pradesh, 208 024, India

    Sanjeev Gupta

  2. Molecular Breeding Laboratory, Division of Crop Improvement, Indian Institute of Pulses Research (Indian Council of Agricultural Research), Kanpur, Uttar Pradesh, 208 024, India

    Debjyoti Sen Gupta, Tuba K. Anjum, Aditya Pratap & Jitendra Kumar

Authors
  1. Sanjeev Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Debjyoti Sen Gupta
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tuba K. Anjum
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Aditya Pratap
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jitendra Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sanjeev Gupta.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gupta, S., Gupta, D.S., Anjum, T.K. et al. Inheritance and molecular tagging of MYMIV resistance gene in blackgram (Vigna mungo L. Hepper). Euphytica 193, 27–37 (2013). https://doi.org/10.1007/s10681-013-0884-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-013-0884-4

Keywords

Search

Navigation