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QTL for spikelet number from a high-yielding rice variety, Hoshiaoba, detected in an introgression line with the genetic background of an indica rice variety, IR64

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Abstract

Total spikelet number per panicle (TSN) is thought to be one of the most important agronomic traits associated with grain production in rice (Oryza sativa L.). We previously reported the development of 334 introgression lines (ILs) with variations in agronomic traits in the genetic background of indica rice variety IR64. Among these, an IL derived from high-yielding rice variety Hoshiaoba showed significantly higher TSN than IR64. We therefore have conducted a quantitative trait locus (QTL) analysis for TSN in an F2 population of IL × IR64 to ascertain the genetic basis for the high TSN of the IL. The analysis revealed the presence of a QTL for TSN on chromosome 7, where several QTLs for grain number have been previously reported. We developed a near-isogenic line (NIL) for this QTL by using DNA marker-assisted selection and characterized its effect. The NIL showed significantly higher TSN than IR64. These results suggest that the QTL and the NIL will be useful materials in breeding programs aimed at increasing TSN.

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Acknowledgments

This paper reports the results obtained in the IRRI–Japan Collaborative Research Project (phases III, IV, V, and VI), which was funded by the Ministry of Agriculture, Forestry and Fisheries through the Ministry of Foreign Affairs of Japan. This work was partially supported by Research Fellowships from the Japan Society for the Promotion of Science for Young Scientists (22-6429 for Y.K. and 23-7274 for D.F.).

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Author notes

  1. Daisuke Fujita & Nobuya Kobayashi

    Present address: National Institute of Crop Science (NICS), National Agriculture and Food Research Organization (NARO), 2-1-18 Kannondai, Tsukuba, Ibaraki, 305-8518, Japan

Authors and Affiliations

  1. Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki, 305-8686, Japan

    Yohei Koide, Kazuhiro Sasaki, Tsutomu Ishimaru, Yoshimichi Fukuta & Nobuya Kobayashi

  2. International Rice Research Institute (IRRI), DAPO Box 7777, Metro Manila, Philippines

    Yohei Koide, Daisuke Fujita, Analiza G. Tagle, Kazuhiro Sasaki, Tsutomu Ishimaru & Nobuya Kobayashi

Authors
  1. Yohei Koide
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  2. Daisuke Fujita
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  3. Analiza G. Tagle
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  4. Kazuhiro Sasaki
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  5. Tsutomu Ishimaru
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  6. Yoshimichi Fukuta
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  7. Nobuya Kobayashi
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Correspondence to Nobuya Kobayashi.

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Koide, Y., Fujita, D., Tagle, A.G. et al. QTL for spikelet number from a high-yielding rice variety, Hoshiaoba, detected in an introgression line with the genetic background of an indica rice variety, IR64. Euphytica 192, 97–106 (2013). https://doi.org/10.1007/s10681-013-0882-6

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  • DOI: https://doi.org/10.1007/s10681-013-0882-6

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