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Mapping of QTLs in tomato line FLA456 associated with resistance to a virus causing tomato yellow leaf curl disease

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Abstract

Tomato (yellow) leaf curl disease (TYLCD) is a serious threat to tomato production in the tropics and subtropics. The genetics of resistance to Tomato yellow leaf curl Thailand virus Taiwan strain (TYLCTHV-[TW]) in a highly resistant tomato line FLA456 was studied through quantitative trait loci (QTL) analysis. Four QTLs named qTy4.1, qTy6.1, qTy10.1 and qTy11.1 were detected on chromosomes 4, 6, 10, and 11, respectively, through evaluation of an F6 recombinant inbred line (RIL) population derived from a cross between FLA456 (resistant) and CLN1621L (susceptible). Gene action of all QTLs was recessive based on disease reaction of the F1. The markers SINAC1 and SLM4-34 flanked qTy4.1 on chromosome 4, and SLM11-12 and SLM11-17 defined qTy11.1, which co-located with the previously identified Ty-5 and Ty-2 loci, respectively. qTy6.1 was flanked by the markers SLM6-55 and TES-0014, and qTy10.1 by the markers SLM10-80-SLM10-46 on chromosomes 6 and 10. The LOD values of the putative QTLs ranged from 2.79 to 13.76. The phenotypic variance explained by each QTL ranged from 7.1 to 31.9 %. The four QTLs collectively contributed about 60.5 % of the phenotypic variation in resistance against TYLCTHV-[TW]. Group mean severity scores of those RILs possessing three or four qTy were generally lower than RIL groups with only one or no qTy. Given the diversity of begomoviruses that cause TYLCD across the regions, the new QTLs from FLA456 would be valuable in tomato breeding for developing varieties with durable resistance. Two QTL intervals (qTy4.1 and qTy10.1) contained virus resistance candidate genes such as CTV.22 and eukaryotic translation initiation factor 4E.

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Acknowledgments

The authors thank Julie Chu for technical support, Fang-I Ho and Eguru Sreenivasa Rao for providing SSR primer pairs, Dolores Ledesma for statistical assistance, Maureen Mecozzi for editing the manuscript, and Professor John Scott for providing seed of FLA456. Financial support from the Federal Ministry for Economic Cooperation and Development, Germany, project number 03.7860.4-001.00 and the Asia and Pacific Seed Association (APSA-Bangkok) is gratefully acknowledged.

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Author notes

  1. P. Kadirvel

    Present address: Directorate of Oilseeds Research, Indian Council of Agricultural Research, Rajendranagar, Hyderabad, 500030, India

  2. S. Geethanjali

    Present address: Coconut Research Station, Tamil Nadu Agricultural University, Aliyarnagar, Pollachi, Coimbatore, 642101, India

  3. R. de la Peña

    Present address: East-West Seed, 50/1 Moo 2, Sanoi-Bangbuathong Road, Sainoi, Nonthaburi, 11150, Thailand

Authors and Affiliations

  1. AVRDC—The World Vegetable Center, PO Box 42, Shanhua, Tainan, 74199, Taiwan

    P. Kadirvel, R. de la Peña, R. Schafleitner, S. Huang, S. Geethanjali, L. Kenyon, W. Tsai & P. Hanson

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Correspondence to P. Hanson.

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Kadirvel, P., de la Peña, R., Schafleitner, R. et al. Mapping of QTLs in tomato line FLA456 associated with resistance to a virus causing tomato yellow leaf curl disease. Euphytica 190, 297–308 (2013). https://doi.org/10.1007/s10681-012-0848-0

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