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Reaction of sugar beet S1 lines and cultivars to different isolates of Macrophomina phaseolina and Rhizoctonia solani AG-2-2IIIB

Euphytica volume 190, pages 439–445 (2013)Cite this article

Abstract

Interactions of 17 sugar beet lines and cultivars with four isolates of Macrophomina phaseolina (the causal agent of charcoal rot) and one isolate of Rhizoctonia solani (the causal agent of crown and root rot) were studied in separate experiments under greenhouse conditions. The isolates of Macrophomina were taken from their host plants, sugar beet (two isolates), soybean and sesame. In the first experiment, the colonized toothpick was used as inoculum. In the second experiment, six-month-old sugar beet plants were inoculated with barley seeds colonized with M. phaseolina. For the inoculation of sugar beet lines with R. solani, the colonized corn seeds were used. Root symptoms were recorded four weeks after inoculation, by estimating the proportion of the root surface infected by the pathogens, using a 1–9 standard scale. Our results showed a significant difference among lines and cultivars in their resistance to these two pathogens. Line B8618 was found to be considerably resistant to the isolates of the both pathogens. The inoculation methods of Macrophomina isolates had no significant effect on the results. The interaction between isolate and cultivar was not also significant in Macrophomina-resistant lines. Therefore, it appears that the response of sugar beet lines to the tested fungal isolates was not differential. These resistant lines showed a high resistance to all the tested M. phaseolina isolates. Our results revealed that the Macrophomina-resistant lines also showed resistance to R. solani. Furthermore, the sugar beet drought tolerant lines (M293, M362 and M345) were susceptible to the tested M. phaseolina and R. solani isolates.

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Acknowledgments

The author is grateful to Iran National Science Foundation for financial support of the study. The drought tolerant S1 lines were kindly supplied by Dr. Masoud Ahmadi. The author is thankful to Dr. Abazar Rajabi and Dr. Omid Eini for the critical review of the manuscript.

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Authors and Affiliations

  1. Sugar Beet Seed Institute, Karaj, Alborz Iran, Islamic Republic of Iran

    Seyed Bagher Mahmoudi

  2. Islamic Azad University, Science and Research Branch, Tehran, Iran

    Sara Ghashghaie

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  1. Seyed Bagher Mahmoudi
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  2. Sara Ghashghaie
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Correspondence to Seyed Bagher Mahmoudi.

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Mahmoudi, S.B., Ghashghaie, S. Reaction of sugar beet S1 lines and cultivars to different isolates of Macrophomina phaseolina and Rhizoctonia solani AG-2-2IIIB. Euphytica 190, 439–445 (2013). https://doi.org/10.1007/s10681-012-0832-8

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  • DOI: https://doi.org/10.1007/s10681-012-0832-8

Keywords

  • Charcoal rot
  • Drought
  • Rhizoctonia solani
  • Sugar beet