Validation of quantitative trait loci for aluminum tolerance in Chinese wheat landrace FSW
- 340 Downloads
Aluminum (Al) toxicity is one of the major constraints for wheat production in acidic soils worldwide and use of Al-tolerant cultivars is one of the most effective approaches to reduce Al damage in the acidic soils. A Chinese landrace, FSW, shows a high level of tolerance to Al toxicity and a mapping population of recombinant inbred lines (RILs) was developed from a cross between FSW and Al-sensitive US spring wheat cultivar Wheaton to validate the quantitative trait loci (QTL) previously identified in FSW. The mapping population was evaluated for net root growth (NRG) during Al stress in a nutrient solution culture and hematoxylin staining score (HSS) of root tips after Al stress. After 132 simple sequence repeat (SSR) markers from three chromosomes that were previously reported to have the QTLs were analyzed in the population, two QTLs for Al tolerance from FSW were confirmed. The major QTL on chromosome 4DL co-segregated with the Al-activated malate transporter gene (ALMT1), however, sequence analysis of the promoter region (Ups4) of ALMT1 gene indicated that FSW contained a marker allele that is different from the one that was reported to condition Al tolerance in the Brazilian source. Another QTL on chromosome 3BL showed a minor effect on Al tolerance in the population. The two QTLs accounted for about 74.9 % of the phenotypic variation for HSS and 72.1 % for NRG and demonstrated an epistatic effect for both HSS and NRG. SSR markers closely linked to the QTLs have potential to be used for marker-assisted selection (MAS) to improve Al tolerance in wheat breeding programs.
KeywordsChinese landrace Aluminum tolerance Simple sequence repeats QTL mapping
This project was partially supported by the National Research Initiative Competitive Grants CAP project 2011-68002-30029 from the USDA National Institute of Food and Agriculture and the scholarship to the first author from State Administration of Foreign Experts Affairs, China (no. CG2008320006). The authors would like to thank Dr. Paul St. Amand, USDA Central Small Grain Genotyping Center, and Dr. Chengsong Zhu, Department of Agronomy, Kansas State University, Manhattan KS, for technical assistance. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer. This is contribution no. 12-413-J from the Kansas Agricultural Experiment Station, Manhattan, KS.
- Aniol A, Gustafson JP (1984) Chromosome locations of genes controlling aluminum tolerance in wheat, rye, and triticale. Can J Genet Cytol 26:701–705Google Scholar
- Bot AJ, Nachtergaele FO, Young A (2000) Land resource potential and constraints at regional and country levels. Food and Agricultural Organization of the United Nations, Rome, pp 1–114Google Scholar
- Garvin DF, Carver BF (2003) Role of genotypes tolerant of acidity and aluminum toxicity. In: Rengel Z (ed) Handbook of soil acidity. Marcel Dekker, New York, pp 387–406Google Scholar
- Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175Google Scholar
- Raman H, Zhang KR, Cakir M, Appels R, Garvin DF, Maron LG, Kochian LV, Moroni JS, Raman R, Imtiaz M, Drake-Brockman F, Waters I, Martin P, Sasaki T, Yamamoto Y, Matsumoto H, Hebb DM, Delhaize E, Ryan (2005) Molecular characterization and mapping of ALMT1, the aluminium-tolerance gene of bread wheat (Triticum aestivum L.). Genome 48:781–791PubMedCrossRefGoogle Scholar
- Raman H, Ryan PR, Raman R, Stodart BJ, Zhang KL, Martin P, Wood R, Sasaki T, Yamamoto Y, Mackay M, Hebb DM, Delhaize E (2008) Analysis of TaALMT1 traces the transmission of aluminum resistance in cultivated common wheat (Triticum aestivum L.). Theor Appl Genet 116:343–354PubMedCrossRefGoogle Scholar
- van Ooijen JW, Voorrips RE (2001) JoinMap® 3.0. Software for the calculation of genetic linkage maps. Plant Res. Int, WageningenGoogle Scholar
- von Uexküll HR, Mutert E (1995) Global extent, development and economic impact of acid soils. In: Date RA, Grundon NJ, Rayment GE, Probert ME (eds) Plant-soil interactions at low pH: principles and management. Kluwer Academic Publisher, BostonGoogle Scholar
- Wang S, Basten CJ, Zeng ZB (2007) Windows QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh. http://statgen.ncsu.edu/qtlcart/WQTLCart.htm