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Screening for new sources of resistance to Clavibacter michiganensis subsp. michiganensis (Cmm) in tomato

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An Erratum to this article was published on 06 November 2012

Abstract

Bacterial canker of tomato, caused by Clavibacter michiganensis subsp. michiganensis (Cmm), is considered the most serious bacterial threat, resulting in high damages in production areas. Worldwide, Cmm is subjected to quarantine regulations.There is no cultivar in market containing Cmm resistance genes. This project aimed to screen tomatoes or wild relatives of tomato for resistance to Cmm, to be used for starting breeding programs. We have screened 24 different wild accessions of tomato and found several new tolerant sources: Solanum pimpinellifolium GI.1554, S. parviflorum LA735 and S. parviflorum LA2072. We also confirmed the tolerance which was reported previously in S. peruvianum LA2157, S. peruvianum PI127829, S. peruvianum LA385, S. habrochaites LA407 and S. lycopersicum cv. IRAT L3. No immunity was found. Also accessions showing a low disease score still contained high titers of bacteria as determined by a dilution plating method, using tow selective media. These results were confirmed with a TaqMan real time PCR assay, which was developed to determine and quantify Cmm in planta.

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Acknowledgments

We would like to thank Patricia van der Zouwen and Marjon Krijger from Wageningen University Plant Research International Biointeraction and Plant Health group for help, and the Scientific and Technological Research Council of Turkey (TÜBİTAK) for financial support.

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Authors and Affiliations

  1. Department of Plant Breeding, Wageningen University and Research Centre, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands

    Yusuf Sen, Zhu Feng, Henri Vandenbroucke, Richard G. F. Visser & A. W. van Heusden

  2. Plant Research International Biointeractions and Plant Health, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Jan van der Wolf

  3. Graduate School Experimental Plant Sciences, Wageningen, The Netherlands

    Yusuf Sen

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  1. Yusuf Sen
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  2. Zhu Feng
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  3. Henri Vandenbroucke
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  4. Jan van der Wolf
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  5. Richard G. F. Visser
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  6. A. W. van Heusden
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Correspondence to Yusuf Sen.

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Sen, Y., Feng, Z., Vandenbroucke, H. et al. Screening for new sources of resistance to Clavibacter michiganensis subsp. michiganensis (Cmm) in tomato. Euphytica 190, 309–317 (2013). https://doi.org/10.1007/s10681-012-0802-1

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