, Volume 188, Issue 2, pp 153–162 | Cite as

Screening and selection of lentil (Lens Miller) germplasm resistant to seed bruchids (Bruchus spp.)

  • Irene Laserna-RuizEmail author
  • Marcelino De-Los-Mozos-PascualEmail author
  • Omar Santana-Méridas
  • Raúl Sánchez-Vioque
  • María Fernanda Rodríguez-Conde


Seed weevil (Bruchus spp.) is one of the most damaging pests to lentil crop, and the current chemical treatments to control this insect present numerous disadvantages. In this work, we have searched for resistant or almost resistant lentil cultivars to be used as potential sources of genes for resistance to bruchids as a more sustainable and environmentally friendly approach to diminish harvest loss. Thus, a lentil germplasm collection of 571 accessions from 27 different countries, and including wild species was screened for susceptibility to seed bruchids under natural field conditions in Central Spain. Infestation was evaluated in comparison with a check (lentil cultivar ‘Lyda’) according to the number of eggs per pod and the infestation rate in seeds (IR). A large variation of IR (0–70 %) was observed among accessions although, in general, wild species were consistently less affected by bruchids than the check. A total of 32 accessions, including Lens culinaris Medikus subsp. culinaris, Lens culinaris Medikus subsp. orientalis (Boiss.) Ponert, Lens nigricans (M. Bieb.) Godr., and Lens lamottei Cezfr., showed lower infestation rates than the check and were selected as potential sources of resistance to this pest. Potential mechanisms of resistance of selected accessions and their inclusion in a breeding program are outlined.


Lentil Lens spp. Screening Pest resistance Bruchus spp. Germplasm evaluation 



This study is a part of the project PAI09-0009-1064, which was funded by the Consejería de Educación y Ciencia, Junta de Comunidades de Castilla-La Mancha. Irene Laserna Ruiz was supported by a fellowship from Consejería de Agricultura, Junta de Comunidades de Castilla-La Mancha. Additional financing was provided by the European Social Fund. The authors are in debt to the Banco de Germoplasma Vegetal de Cuenca, BGV-CU (Cuenca, Spain), for supplying the plant material. We are grateful to Jose Vicente Reina Ureña, Miguel Ángel Escolano Tercero, Jose Miguel García Poves and Antonio Valiente Martínez for providing technical assistance.

Supplementary material

10681_2012_752_MOESM1_ESM.pdf (134 kb)
Supplementary material (PDF 135 kb)


  1. Barulina H (1930) Lentils of the USSR and other countries. Trudy Prikl Bot Seleck Suppl 40:1–319Google Scholar
  2. Beniwal SPS, Bayaa B, Weigand S, MakkouK KH, Saxena MC (1993) Field guide to lentil diseases and insect pests.
  3. Chopra N, Pajni HR (1987) Resistance of different lentil varieties to the attack of Bruchus lentis. Lens Newslett 23(1/2):45–48Google Scholar
  4. Clement SL (2002) Insect resistance in the wild relatives of food legumes and wheat. In: McComb JA (eds) Plant breeding for the 11th millennium. Proceedings of the 12th Australasian plant breeding conference, Perth, WA, 15–20 Sept 2002Google Scholar
  5. Clement SL, El-Din Sharaf El-Din N, Weigand S, Lateef SS (1994) Research achievements in plant resistance to insect pests of cool season food legumes. Euphytica 73:41–50CrossRefGoogle Scholar
  6. Clement SL, McPhee KE, Elberson LR, Evans MA (2009) Pea weevil, Bruchus pisorum L. (Coleoptera: Bruchidae), resistance in Pisum sativum × Pisum fulvum interspecific crosses. Plant Breed 128:478–485CrossRefGoogle Scholar
  7. Cochran WG, Cox GM (1992) Experimental designs. Wiley, New YorkGoogle Scholar
  8. Cubero JI, Pérez de la Vega M, Fratini R (2009) Origin, phylogeny, domestication and spread. In: Erskine W, Muehlbauer FJ, Sharker A (eds) The lentil: botany, production and uses. CABI, UK, pp 13–33CrossRefGoogle Scholar
  9. Delobel A, Delobel B (2003) Les plantes hôtes des bruches (Coleoptera Bruchidae) de la faune de France, une analyse critique. Bull Mens Soc Linn Lyon 72(6):199–221Google Scholar
  10. Delobel A, Delobel B (2005) Les plantes hôtes des bruches (Coleoptera Bruchidae): données nouvelles et corrections. Bull Mens Soc Linn Lyon 74(7–8):277–291Google Scholar
  11. Domingues SJS, Melo FR, Aguiar JM, Affonso AG, Giulli JSA, Rose JL, Sales MP, Machado LF, Azevedo CR, Da Cunha PC, Ferreira-Uchoa A, Amâncio de Oliveira AE, Xavier-Filho J, Fernandes KVS (2006) Resistance of Vigna unguiculata (cowpea) seeds to Callosobruchus maculatus is restricted to cotyledonary tissues. J Sci Food Agric 86:1977–1985CrossRefGoogle Scholar
  12. Doss RP, Oliver JE, Proebsting WM, Potter SW, Kuy SR, Clement SL, Williamson RT, Carney JR, DeVillbiss ED (2000) Bruchins: insect-derived plant regulators that stimulate neoplasm formation. PNAS 97:6218–6223PubMedCrossRefGoogle Scholar
  13. Edwards O, Singh KB (2006) Resistance to insect pests: what do legumes have to offer? Euphytica 147:273–285CrossRefGoogle Scholar
  14. Erskine W, Tufail M, Russell A, Tyagi MC, Rahman MM, Saxena MC (1994) Current and future strategies in breeding lentil for resistance to biotic and abiotic stresses. Euphytica 73:127–135CrossRefGoogle Scholar
  15. Erskine W, Sarker A, Kumar S (2011) Crops that feed the world 3. Investing in lentil improvement toward a food secure word. Food Sec 3:127–139CrossRefGoogle Scholar
  16. Ferguson ME, Maxted N, Van Slageren M, Robertson LD (2000) A reassessment of the taxonomy of Lens Miller (Leguminosae, Papilionoideae, Vicieae). Bot J Linn Soc 133:41–59CrossRefGoogle Scholar
  17. Food and Agriculture Organization of the United Nations (FAO)/World Health Organization (WHO), Codex Alimentarius (1995)
  18. Food and Agriculture Organization of the United Nations, FAOStat (2010)
  19. Fratini R, Ruiz ML (2006) Interspecific hybridization in the genus Lens applying in vitro embryo rescue. Euphytica 150:271–280CrossRefGoogle Scholar
  20. Fratini R, Ruiz ML, Pérez de la Vega M (2004) Intra-specific and inter-sub-specific crossing in lentil (Lens culinaris Medik.). Can J Plant Sci 84:981–986CrossRefGoogle Scholar
  21. Gulati A, Schryer P, Mchughen A (2002) Production of fertile transgenic lentil (Lens culinaris Medik.) plants using particle bombardment. In Vitro Cell Dev Biol Plant 38:316–324CrossRefGoogle Scholar
  22. Gupta D, Sharma SK (2007) Widening the gene pool of cultivated lentils through introgression of alien chromatin from wild Lens subspecies. Plant Breed 126:58–61CrossRefGoogle Scholar
  23. Hardie DC, Baker GJ, Marshall DR (1995) Field screening of Pisum accessions to evaluate their susceptibility to the pea weevil (Coleoptera: Bruchidae). Euphytica 84:155–161CrossRefGoogle Scholar
  24. Hussain S, Nasir M, Bretag T (2000) Lentil germplasm evaluation for resistance against Ascochyta blight caused by Ascochyta lentis. Pak J Agric Res 16(2):118–121Google Scholar
  25. Hussain S, Ali A, Zahid MA, Iqbal SM (2008) Multiple disease resistance in lentil germplasm from Pakistan. Pak J Bot 40(2):897–903Google Scholar
  26. International Plant Genetic Resouces Institute (IPGRI) (2001) The design and analysis of evaluation trials of genetic resources collections. A guide for genebank managers. IPGRI Technical Bulletin No. 4. International Plant Genetic Resources Institute, Rome, ItalyGoogle Scholar
  27. Jermy T, Szentesi Á (2003) Evolutionary aspects of host plant specialisation—a study on bruchids (Coleoptera: Bruchidae). Oikos 101:196–204CrossRefGoogle Scholar
  28. Kogan M (1982) Plant resistance in pest management. In: Metcalf RL, Luckmann WH (eds) Introduction to insect pest management. Wiley, New York, pp 93–134Google Scholar
  29. Kumar Y, Mishra SK, Tyagi MC, Singh SP, Sharma B (2005) Linkage between genes for leaf colour, plant pubescence, number of leaflets and plant height in lentil (Lens culinaris Medik.). Euphytica 145:41–48CrossRefGoogle Scholar
  30. Ladizinsky G, Braun D, Goshen D, Muehlbauer FJ (1984) The biological species of the genus Lens L. Bot Gaz 154:253–261CrossRefGoogle Scholar
  31. Lattanzio V, Terzano R, Cicco N, Cardinali A, Di Venere D, Linsalata V (2005) Seed coat tannins and bruchid resistance in stored cowpea seeds. J Sci Food Agric 85:839–846CrossRefGoogle Scholar
  32. Ministerio de Medio Ambiente y Medio Rural y Marino (MARM) (2010) Anuario de estadística 2010.
  33. Morton RL, Schroeder HE, Bateman KS, Chrispeeld MJ, Armstrong E, Higgins TJV (2000) Bean α-amylase inhibitor 1 in transgenic peas (Pisum sativum) provides complete protection from pea weevil (Bruchus pisorum) under field conditions. PNAS 97(8):3820–3825PubMedCrossRefGoogle Scholar
  34. Mozos-Pascual M (1992) Brúquidos (Coleoptera: Bruchidae) asociados al cultivo de la lenteja (Lens culinaris Medikus) en Castilla-La Mancha: especies implicadas y valoración de la plaga. Bol Sanid Veg Plagas 18:347–353Google Scholar
  35. Mozos-Pascual M (1999) Resultados de Investigación Agraria. El falso gorgojo de la lenteja: biología y control. Hoja informativa no 20. Dirección General de Desarrollo Rural. Servicio de Investigación y Tecnología Agraria. Junta de Comunidades de Castilla-La Mancha, ToledoGoogle Scholar
  36. Mozos-Pascual M, Hernández M (1995) Evaluación de la susceptibilidad al ataque de brúquidos (Coleoptera: Bruchidae) y otros caracteres agronómicos en germoplasma del género Lens (Leguminosae). In: XIV Jornadas de la Asociación española de Entomología, Cuenca, 3–7 julio 1995, p 172Google Scholar
  37. Muehkbauer FJ, Kaiser WJ (1994) Using host plant resistance to manage biotic stresses in cool season food legumes. Euphytica 73:1–10CrossRefGoogle Scholar
  38. Muehlbauer FJ (1993) Use of wild species as a source of resistance in cool-season food legume crops. In: Singh KB, Saxena MC (eds) Breeding for stress tolerance in cool-season food legumes. Wiley, UK, pp 359–372Google Scholar
  39. Muehlbauer FJ, Slinkard AE (1981) Genetics and breeding methology. In: Webb C, Hawtin G (eds) Lentils. CABI, UK, pp 69–90Google Scholar
  40. Muehlbauer FJ, Kaiser WJ, Clement SL, Summerfield RJ (1995) Production and breeding of lentil. Adv Agron 54:283–332CrossRefGoogle Scholar
  41. Muehlbauer FJ, Cho S, Sarker A, McPhee KE, Coyney CJ, Rajesh PN, Ford R (2006) Application of biotechnology in breeding for resistance to biotic and abiotic stress. Euphytica 147:149–165CrossRefGoogle Scholar
  42. Painter RH (1951) Insect resistance in crop plants. University of Kansas Press, Lawrence, p 520Google Scholar
  43. Pajni HR, Mittal K (1984) Observations on infestation or Lens culinaris by Bruchus lentis in the Chandigarh area of India. Lens Newslett 11(1):27–28Google Scholar
  44. Pajni HR, Sidhu NS, Girdhar AK (1996) Infestation of Lens culinaris Medik. by Bruchus lentis Froel. as affected by cropping pattern and other practices in Chandigarh Area, India. Lens Newslett 23(1/2):45–48Google Scholar
  45. Pereira M, Rodrigues I, Grossi-de-Sá MF, Xavier-Filho J (2000) Do legume storage proteins play a role in defending seeds against bruchids? Plant Physiol 124:515–522CrossRefGoogle Scholar
  46. Price PW (1991) The plant vigour hypothesis and herbivore attack. Oikos 62:244–251CrossRefGoogle Scholar
  47. Roberts EH, Summerfield RJ, Muehlbauer FJ, Short RW (1986) Flowering in lentil (Lens culinaris Medic.): the duration of the photoperiodic inductive phase as a function of accumulated daylength above the critical photoperiod. Ann Bot 58:235–248Google Scholar
  48. Sarker RH, Biswas A, Mustafa BM, Mahbub S, Hoque MI (2003) Agrobacterium-mediated tansformation of gentil (Lens culinaris Medik.). Plant Tissue Cult 13(1):1–12Google Scholar
  49. Stevenson PC, Dhillon MK, Sharma HC, El Bouhssini M (2007) Insect pests of lentil and their management. In: Yadav SS, McNeil D, Stevenson PC (eds) Lentil. An ancient crop for modern times. Springer, The Netherlands, pp 342–343Google Scholar
  50. Szentesi Á, Jermy T (1995) Predispersal seed predation in leguminous species: seed morphology and bruchid distribution. Oikos 73:23–32CrossRefGoogle Scholar
  51. Tomooka N, Kashiwaba K, Vaughan DA, Ishimoto M, Egawa Y (2000) The effectiveness of evaluating wild species: searching for sources of resistance to bruchid beetles in the genus Vigna subgenus Ceratotropis. Euphytica 115:27–41CrossRefGoogle Scholar
  52. Tullu A, Diederichsen A, Suvorova G, Vandenberg A (2010) Genetic and genomic resources of lentil: status, use and prospects. Plant Genet Resour Charact Util 9(1):19–29CrossRefGoogle Scholar
  53. Ujagir R, Byrne OMT (2009) Insect pests and their management. In: Erskine W, Muehlbauer FJ, Sharker A (eds) The lentil: botany, production and uses. CABI, UK, pp 282–305Google Scholar
  54. Yus-Ramos R (2007) Genera de Coleópteros de la Península Ibérica e Islas Baleares: familia Bruchidae (Coleoptera, Chrysomeloidea). Bol Asoc Esp Entomol 31(1–2):65–114Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Irene Laserna-Ruiz
    • 1
    Email author
  • Marcelino De-Los-Mozos-Pascual
    • 1
    Email author
  • Omar Santana-Méridas
    • 1
    • 2
  • Raúl Sánchez-Vioque
    • 1
    • 2
  • María Fernanda Rodríguez-Conde
    • 1
  1. 1.Centro Agrario de Albaladejito, Junta de Comunidades de Castilla-La ManchaCuencaSpain
  2. 2.Instituto de Recursos Humanos para la Ciencia y la Tecnología (INCRECYT), Fundación Parque Científico y Tecnológico de AlbaceteAlbaceteSpain

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