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Transfer of architectural traits from perennial Helianthus mollis Lam. to sunflower (H. annuus L.) and localisation of introgression

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Abstract

Common sunflower (i.e., Helianthus annuus) is occasionally crossed with other Helianthus species to gain favourable agronomic traits. An interspecific cross between H. annuus and Helianthus mollis was used to examine events that occurred during the five first generations. First generation hybrid plants were verified using GISH, and F1 hybrid (#17) was back-crossed to common sunflower to obtain BC1 (#4) and BC2 (#21). Some BC2 progeny possessed new traits, i.e., compact (Compact plant architecture is due to short inter-node length, short petiole length and short leaf lamina length) architecture without and with corrugated leaves, which were not observed in the parents. These traits were selected for and fixed after the BC2-S3 (#18) to produce a H. mollis introgression line (namely HM374). Although the size of the population was small, we tentatively construct a genetic map using data from the BC2 generation. Two linkage groups were identified carrying H. mollis specific AFLP markers that were associated with compact plant architecture, and one unlinked marker was shown to explain variation for the corrugated leaves. Introgression line HM374 was used to test the inheritance of compact plant architecture in a cross with an elite high oleic line, OL75HO. Microsatellite (SSR) markers were used to genotype F1 and F2 offspring’s. Our data suggested that the line HM374 was still heterozygous for introgression after eight generations. HM374 displays new architecture traits (i.e. compact architecture with or without corrugated leaves) and carries H. mollis fragments anchored on LG1 and LG11 of sunflower public map.

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Acknowledgments

Marie Coque presently in Biogemma Inc contributed to this work by 2003. Thanks are due to Jessica Barb (The University of Georgia) for English improvements and valuable suggestions and to the sunflower team in Mauguio: Bruno Lorentz, Jean-Paul Boudon, and Catherine Weinachter-Kahane who provided excellent technical assistance..

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  1. Catherine Breton

    Present address: INRA UMR-AGAP, Equipe Davem Campus Supagro Bât 22, 34060, Montpellier Cedex 1, France

Authors and Affiliations

  1. The University of Georgia, Center for Applied Genetic Technologies, 111 Riverbend Road, Athens, GA, 30602, USA

    Catherine Breton

  2. INRA UMR 1097, Campus Supagro, 2 Place Viala, Bât 33, 34060, Montpellier Cedex 1, France

    Julie Wargnier & André Bervillé

  3. INRA UMR 1097, Chemin Du Mézouls, 34130, Mauguio, France

    Alain Gil & Hervé Serieys

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  1. Catherine Breton
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Correspondence to Catherine Breton.

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Breton, C., Gil, A., Wargnier, J. et al. Transfer of architectural traits from perennial Helianthus mollis Lam. to sunflower (H. annuus L.) and localisation of introgression. Euphytica 186, 557–572 (2012). https://doi.org/10.1007/s10681-012-0656-6

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