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Development of a coupling-phase SCAR marker linked to the powdery mildew resistance gene ‘er1’ in pea (Pisum sativum L.)

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Abstract

Pea powdery mildew is one of the major constraints in pea production worldwide, causing severe seed yield and quality loss. The resistance is governed by a single recessive gene er1 in majority of resistant cultivars, but er2 and Er3 have also been reported. The objective of the study was to find out tightly linked sequence characterized amplified regions (SCAR) markers to er1 gene using NILs. A total of 620 random amplified polymorphic DNA (RAPD) markers were screened for length polymorphism between seven sets of NILs. The 880 bp polymorphic band of the tightly linked RAPD marker OPX 04880 was cloned, sequenced and a SCAR marker ScOPX 04880 was developed. In a population of completely classified 208 F2 plants (supported by phenotypic data from 208 F2:3 and 4,390 F3:4 families) ScOPX 04880 was linked at 0.6 cM in coupling phase with er1 gene in the order ScOPX 04880–er1–ScOPD 10650. ScOPX 04880 will correctly differentiate homozygous resistant plants from the susceptible accessions with more than 99 % accuracy. In combination with repulsion phase marker ScOPD 10650, ScOPX 04880 can help in an error free marker-assisted selection.

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Notes

  1. Superscript indicates the donor parent for powdery mildew susceptible (PMS) or resistant (PMR) trait.

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Acknowledgments

The authors thank Dr. N.K. Singh, National Research Centre on Plant Biotechnology (NRCPB), New Delhi, India for his help and valuable suggestions in the course of the study. Rakesh K. Srivastava gratefully acknowledges SRF grant from the Council of Scientific and Industrial Research (CSIR), India; and IARI fellowship from the Indian Agricultural Research Institute (IARI), New Delhi, India.

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  1. Rakesh K. Srivastava

    Present address: Global Theme-Biotechnology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Building 305, Patancheru, Hyderabad, 502324, Andhra Pradesh, India

Authors and Affiliations

  1. Division of Genetics, Indian Agricultural Research Institute (IARI), Pusa Campus, New Delhi, 110012, India

    Rakesh K. Srivastava, Shashi K. Mishra & Ashok K. Singh

  2. Division of Germplasm Evaluation, National Bureau of Plant Genetic Resources (NBPGR), Pusa Campus, New Delhi, 110012, India

    Shashi K. Mishra

  3. National Research Centre on Plant Biotechnology (NRCPB), Indian Agricultural Research Institute (IARI), Pusa Campus, New Delhi, 110012, India

    Trilochan Mohapatra

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  1. Rakesh K. Srivastava
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Correspondence to Rakesh K. Srivastava.

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Srivastava, R.K., Mishra, S.K., Singh, A.K. et al. Development of a coupling-phase SCAR marker linked to the powdery mildew resistance gene ‘er1’ in pea (Pisum sativum L.). Euphytica 186, 855–866 (2012). https://doi.org/10.1007/s10681-012-0650-z

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