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Assessment of the uniformity and stability of grapevine cultivars using a set of microsatellite markers

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Abstract

Solidity of microsatellite markers is a key issue for varietal identification, especially when they are used for legal purposes, what includes their probable future use in the distinctness, uniformity and stability testing of new varieties needed for the granting of Plant Breeders’ Rights. Nine grapevine microsatellites (VVS2, VVMD5, VVMD27, VVMD28, ssrVrZAG29, ssrVrZAG62, ssrVrZAG67, ssrVrZAG83 and ssrVrZAG112), which had previously demonstrated its capacity to discriminate any grapevine variety, have been assessed to evaluate its uniformity and stability. 19 varieties were selected, representative of a high diversity for morphological, agronomical, cultural and historical aspects, as well as for microsatellite allele variability. Then, for each variety, uniformity and stability were evaluated through the analysis of 50 plants from each of three different plots, and five plants from each of seven additional plots. Material from 4,137 plants of 229 plots of the 19 varieties was sampled in seven countries. Of 3,654 plants analyzed with the set of nine microsatellites, 3,299 were of the right variety and used for the survey. An average of 172 individual values was studied for each allele of each microsatellite of each variety, and none differences were detected that could not be explained as technical variations, with the exception of several putative chimeras in two varieties. Of the total of 171 variety x microsatellite combinations, only in one combination (‘Merlot’ x VVMD27) the number of off-types exceeded the threshold allowed. The remaining 170 combinations have been found uniform and stable according to internationally accepted rules.

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Acknowledgments

This study was financially supported by the project VIN01-025 (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Agriculture Ministry of Spain). M.D. Vélez was funded by a pre-doctoral fellowship from the Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario (IMIDRA). The large sampling needed in this work has been possible thanks to the collaboration of numerous people and public and private institutions, in Spain and abroad. We are grateful to all of them. Specifically, plant material from wine varieties was obtained thanks to the collaboration of numerous Regulator Councils of wine Origin Denominations (see Materials and methods). Among the people who personally contributed to the sampling are: José María Hurtado (Superior Frutícola S.A., Murcia, Spain); Edit Hajdu (FVM Szölészeti és Borászati Kutató Intézete, Hungary); Patricio Hinrichsen (Centro Experimental La Platina-INIA, Chile); Tim Sheehan (Sheehan Genetics, USA); Jean Satterwhite (National Clonal Germplasm Repository for Nut Crops, USA); Erika Maul (Institute for Grapevine Breeding, Germany); Jorge Zerolo (Agrovolcán, Tenerife, Spain); Nuria Cid (Estación de Viticultura y Enología de Galicia, Orense, Spain); Peter Allderman (Top Fruit, RSA); Thierry Lacombe (DGPC-Diversité et Génomes des Plantes Cultivées, France); Miguel Lara (CIFA-Centro de Investigación y Formación Agraria, Jerez de la Frontera, Spain); Joaquín Borrego, Paz Fernández, Maite de Andrés, Carlos González, Alba Vargas, Gregorio Muñoz, Cristina Rubio and Mariano Cabellos (IMIDRA, Spain). We apologize for any non-deliberate omission in this list.

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  1. M. Dolores Vélez

    Present address: Departamento de Genética y Ecofisiología Forestal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Carretera A-6, km. 7,5, 28040, Madrid, Spain

  2. Javier Ibáñez

    Present address: Instituto de Ciencias de la Vid y del Vino (CSIC-UR-CAR), Complejo Científico Tecnológico. C/Madre de Dios 51, 26006, Logroño, Spain

Authors and Affiliations

  1. Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario (IMIDRA), Finca El Encín. Carretera A-2, PK 38,200, 28800, Alcalá de Henares, Spain

    M. Dolores Vélez & Javier Ibáñez

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  1. M. Dolores Vélez
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Correspondence to Javier Ibáñez.

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Vélez, M.D., Ibáñez, J. Assessment of the uniformity and stability of grapevine cultivars using a set of microsatellite markers. Euphytica 186, 419–432 (2012). https://doi.org/10.1007/s10681-012-0633-0

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