Abstract
During the last few decades, the physiological and genetic background of dormancy, and correlated pre-harvest sprouting (PHS) have been intensively investigated. Special attention has often been paid to genetic factors that may explain and predict PHS susceptible behaviour. A major candidate is the Vp1 gene which is involved in embryo development and maturation as well as in dormancy establishment. In this study, Vp1 gene expression during kernel development was studied in wheat, triticale and rye as a potential biomarker for selecting PHS tolerant varieties in cereal breeding programs. Plants of known PHS tolerant and PHS susceptible varieties were grown under controlled conditions from flowering until harvest ripeness. During that period, kernels were regularly harvested for RNA extraction and cDNA synthesis. Calibrated and normalized relative Vp1 expression levels were obtained in an RT-qPCR assay. During kernel development, Vp1 expression levels generally showed a typical peak during the soft dough stage, after which they decreased and remained low until harvest maturity. Differences in Vp1 expression levels could be observed between the PHS susceptible and PHS tolerant varieties of wheat, with the PHS tolerant variety showing higher levels of relative Vp1 expression compared to the PHS susceptible variety. In triticale, however, this difference was only seen once and could not be confirmed in further experiments. It seems that the Vp1 gene in triticale behaves in a similar way as in rye, in which no specific trends could be observed.
Similar content being viewed by others
Abbreviations
- ABA:
-
Abscisic acid
- CNRQ:
-
Calibrated and normalized relative quantity
- DM:
-
Dry matter
- DPA:
-
Days post anthesis
- GA:
-
Gibberellic acid
- GI:
-
Germination index
- PHS:
-
Pre-harvest sprouting
- PM:
-
Physiological maturity
- RT-qPCR:
-
Real-time reverse transcriptase polymerase chain reaction
References
Bailey PC, McKibbin RS, Lenton JR, Holdsworth MJ, Flintham JE, Gale MD (1999) Genetic map locations for orthologous Vp1 genes in wheat and rice. Theor Appl Genet 98:281–284
Benech-Arnold R (2004) Inception, maintenance, and termination of dormancy in grain crops: physiology, genetics and environmental control. In: Benech-Arnold R, Sanchez R (eds) Handbook of seed physiology: application to agriculture. Food Products Press, New York, pp 169–198
Biddulph TB, Mares DJ, Plummer JAS, Setter TL (2005) Drought and high temperature increases preharvest sprouting tolerance in a genotype without grain dormancy. Euphytica 143:277–283. doi:10.1007/s10681-005-7882-0
Black M, Bewley JD, Halmer P (2006) The encyclopedia of seeds: science, technology and uses. CAB International, Wallingford
Bradford KJ, Nonogaki H (eds) (2007) Seed development, dormancy and germination. Blackwell Publishing Ltd, Oxford
Carrari F, Perez-Flores L, Lijavetzky D, Enciso S, Sanchez R, Benech-Arnold R, Iusem N (2001) Cloning and expression of a sorghum gene with homology to maize vp1. Its potential involvement in pre-harvest sprouting resistance. Plant Mol Biol 45:631–640
Carrari F, Benech-Arnold R, Osuna-Fernandez R, Hopp E, Sanchez R, Iusem N, Lijavetzky D (2003) Genetic mapping of the Sorghum bicolor Vp1 gene and its relationship with preharvest sprouting resistance. Genome 46:253–258
Chang C, Zhang H, Feng J, Yin B, Si H, Ma C (2010) Identifying alleles of Viviparous-1B associated with pre-harvest sprouting in micro-core collections of Chinese wheat germplasm. Mol Breeding 25:481–490
Chang C, Zhang HP, Zhao QX, Feng JM, Si HQ, Lu J, Ma CX (2011) Rich allelic variations of Viviparous-1A and their associations with seed dormancy/pre-harvest sprouting of common wheat. Euphytica 179:343–353
De Laethauwer S, Messens K, Derycke V, Gryson N, Reheul D, Haesaert G (2006) An approach to developing a marker assisted selection system for tolerance to pre-harvest sprouting in Triticale. In: Botes W, Bores D, Darvey N et al. (eds). 6th International Triticale Symposium, Stellenbosch, pp 39–44
De Laethauwer S, Reheul D, De Riek J, Haesaert G (2009) The use of Vp1 in real time RT-PCR to select for pre-harvest sprouting tolerance in triticale. Euphytica 168:379–384
Fan J, Niu X, Wang Y, Ren G, Zhuo T, Yang Y, Lu BR, Liu Y (2007) Short, direct repeats (SDRs)-mediated post-transcriptional processing of a transcription factor gene OsVP1 in rice (Oryza sativa). J Exp Bot 58:3811–3817. doi:10.1093/jxb/erm231
Giraudat J, Hauge BM, Valon C, Smalle J, Parcy F, Goodman HM (1992) Isolation of the arabidopsis ABI3 gene by positional cloning. Plant Cell 4:1251–1261. doi:10.1105/tpc.4.10.1251
Gualano N, Benech-Arnold R (2009) The effect of water and nitrogen availability during grain filling on the timing of dormancy release in malting barley crops. Euphytica 168:291–301. doi:10.1007/s10681-009-9948-x
Gupta PK, Varshney RK (2000) The development and use of microsatellite markers for genetic analysis and plant breeding with emphasis on bread wheat. Euphytica 113:163–185
Hellemans J, Mortier G, De Paepe A, Speleman F, Vandesompele J (2007) qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol 8:R19
Hess JR, Carman JG, Banowetz GM (2002) Hormones in wheat kernels during embryony. J Plant Physiol 159:379–386
Hoecker U, Vasil IK, McCarty DR (1995) Integrated control of seed maturation and germination programs by activator and repressor functions of Viviparous-1 of maize. Genes Dev 9:2459–2469. doi:10.1101/gad.9.20.2459
Hoecker U, Vasil IK, McCarty DR (1999) Signaling from the embryo conditions Vp1-mediated repression of α-amylase genes in the aleurone of developing maize seeds. Plant J 19:371–377
Hollung K, Espelund M, Schou K, Jakobsen KS (1997) Developmental, stress and ABA modulation of mRNA levels for bZip transcription factors and Vp1 in barley embryos and embryo-derived suspension cultures. Plant Mol Biol 35:561–571
Jones HD, Peters NCB, Holdsworth MJ (1997) Genotype and environment interact to control dormancy and differential expression of the VIVIPAROUS1 homologue in embryos of Avena fatua. Plant J 12:911–920
Koebner RMD (2004) Marker-assisted selection in the cereals: the dream and the reality. In: Gupta PK, Varshney RK (eds) Cereal genomics. Kluwer Academic Publishers, Dordrecht, pp 317–330
McCarty DR, Carson CB, Stinard PS, Robertson DS (1989) Molecular analysis of viviparous-1: an abscisic acid-insensitive mutant of maize. Plant Cell 1:523–532. doi:10.1105/tpc.1.5.523
McKibbin R, Wilkinson M, Bailey P, Flintham J, Andrew L, Lazzeri P, Gale M (2002) Transcripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species. Plant Biol 99:10203–10208
Milligan AS, Lopato S, Langridge P (2004) Functional genomics studies of seed development in cereals. In: Gupta PK, Varshney RK (eds) Cereal genomics. Kluwer Academic Publishers, Dordrecht, pp 447–482
Nakamura S, Toyama T (2001) Isolation of a VP1 homologue from wheat and analysis of its expression in embryos of dormant and non-dormant cultivars. J Exp Bot 52:875–876
Suzuki T, Matsuura T, Kawakami N, Noda K (2000) Accumulation and leakage of abscisic acid during embryo development and seed dormancy in wheat. Plant Growth Regul 30:253–260
Utsugi S, Nakamura S, Noda K, Maekawa M (2008) Structural and functional properties of Viviparous1 genes in dormant wheat. Genes Genet Syst 83:153–166. doi:10.1266/ggs.83.153
Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3:research0034–research0034.11. doi:10.1186/gb-2002-3-7-research0034
Walker-Simmons M (1987) ABA levels and sensitivity in developing wheat embryos of sprouting resistant and susceptible cultivars. Plant Physiol 84:61–66
Wilkinson M, Lenton JR, Holdsworth M (2005) Transcripts of Vp-1 homoeologues are alternatively spliced within the Triticeae tribe. Euphytica 143:243–246. doi:10.1007/s10681-005-7856-2
Wilson I, Barker G, Lu C, Coghill J, Beswick R, Lenton J, Edwards K (2005) Alteration of the embryo transcriptome of hexaploid winter wheat (Triticum aestivum cv. Mercia) during maturation and germination. Funct Integr Genomics 5:144–154
Xia L, Yang Y, Ma Y, Chen X, He Z, Röder M, Jones H, Shewry P (2009) What can the Viviparous-1 gene tell us about wheat pre-harvest sprouting? Euphytica 168:385–394
Yang Y, Ma YZ, Xu ZS, Chen XM, He ZH, Yu Z, Wilkinson M, Jones HD, Shewry PR, Xia LQ (2007a) Isolation and characterization of Viviparous-1 genes in wheat cultivars with distinct ABA sensitivity and pre-harvest sprouting tolerance. J Exp Bot 58:2863–2871. doi:10.1093/jxb/erm073
Yang Y, Zhao XL, Xia LQ, Chen XM, Xia XC, Yu Z, He ZH, Roeder M (2007b) Development and validation of a Viviparous-1 STS marker for pre-harvest sprouting tolerance in Chinese wheats. Theor Appl Genet 115:971–980. doi:10.1007/s00122-007-0624-z
Acknowledgments
The research work of Sarah De Laethauwer was funded by the Research Funding of the University College Ghent. We also thank the colleagues of the Institute for Agricultural and Fisheries Research, Plant Unit, for their technical assistance and expertise.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
10681_2011_613_MOESM1_ESM.tiff
Supplementary material 1 (TIFF 4,105 kb). Online Resource 1 Evolution of dry matter content (%) during kernel development (in days post anthesis) in a pre-harvest sprouting (PHS) tolerant (T, filled square) and a PHS susceptible (S, open diamond) genotype of wheat (a), triticale (b) and rye (c). Arrows indicate physiological maturity (PM)
10681_2011_613_MOESM2_ESM.tiff
Supplementary material 2 (TIFF 1,239 kb). Online Resource 2 Confirming data on Fig. 2a achieved in an additional experiment and representing calibrated and normalized relative Vp1 gene expression (CNRQ) during kernel development as measured by kernel dry matter content (%) of a pre-harvest sprouting (PHS) tolerant (T, filled square) and a PHS susceptible (S, open diamond) genotype of wheat. The insert shows the evolution of dry matter content (%) during kernel development (in days post anthesis) and arrows indicate physiological maturity (PM)
10681_2011_613_MOESM3_ESM.tiff
Supplementary material 3 (TIFF 1,014 kb). Online Resource 3 Visualization of the Vp1 transcripts obtained through RT-qPCR on samples of triticale genotypes AUS20844 (pre-harvest sprouting tolerant) and Ticket (pre-harvest sprouting susceptible) which were separated on 8% polyacrylamide gel. Dry matter percentages of the samples (loaded in duplicate) are indicated at the bottom of the lanes. Lanes without indication: 100 bp DNA ladder (Fermentas)
Rights and permissions
About this article
Cite this article
De Laethauwer, S., Reheul, D., De Riek, J. et al. Vp1 expression profiles during kernel development in six genotypes of wheat, triticale and rye. Euphytica 188, 61–70 (2012). https://doi.org/10.1007/s10681-011-0613-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10681-011-0613-9