Abstract
In this work we address two aspects of eggplant flower biology potentially involved on the efficiency of anther culture: the selection of the best floral stage to extract anthers for culture, and the effect of heterostyly in the identification of suitable buds and anthers. For 12 different accessions, we determined morphological criteria (length ranges) to identify buds and anthers enriched in vacuolate microspores and young bicellular pollen, the stages most responsive to embryogenesis induction. While these microspore/pollen stages were the most responsive when isolated and cultured in liquid medium, we observed that culture of anthers containing these stages is not the best choice. Instead, the highest response was found for younger anthers, containing mostly young and mid microspores. We analyzed eggplant anther walls and found that their particular thickness may be behind this apparent discrepancy, since they may delay the diffusion of inducing factors to the anther locule, reducing their effect over inducible microspores. Thus, the culture of younger anthers would allow for younger microspores to grow up to the inducible stages while factors are entering the locule. We also analyzed the embryogenic response of short and long-styled buds present in Cristal, a heterostylic cultivar. Our results demonstrated that each floral morph produced buds and anthers of different lengths, but equally useful for anther culture, since similar amounts of embryos were produced. The practical application of these results may improve the efficiency of anther culture not only in these cultivars, but in others also presenting thick anther walls and heterostyly.
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References
Alpsoy HC, Seniz V (2007) Researches on the in vitro androgenesis and obtaining haploid plants in some eggplant genotypes. Acta Hortic 729:137–141
Barany I, Gonzalez-Melendi P, Fadón B, Mityko J, Risueño MC, Testillano PS (2005) Microspore-derived embryogenesis in pepper (Capsicum annuum L.): subcellular rearrangements through development. Biol Cell 97:709–722
Beckert M (1998) Genetic analysis of in vitro androgenetic response in maize. In: Chupeau Y, Caboche M, Henry Y (eds) Androgenesis and haploid plants. Springer, Berlin, Heidelberg, pp 24–37
Borgel A, Arnaud M (1986) Progress in eggplant breeding, use of haplomethod. Capsicum Newsl 5:65–66
Dumas de Vaulx R, Chambonnet D (1982) Culture in vitro d’anthères d’aubergine (Solanum melongena L.): stimulation de la production de plantes au moyen de traitements à 35°C associés à de faibles teneurs en substances de croissance. Agronomie 2:983–988
Dunwell JM (2010) Haploids in flowering plants: origins and exploitation. Plant Biotechnol J 8:377–424
Ferrie AMR, Epp DJ, Keller WA (1995) Evaluation of Brassica rapa L genotypes for microspore culture response and identification of a highly embryogenic line. Plant Cell Rep 14:580–584
Gémes Juhasz A, Venczel G, Sagi ZS, Gajdos L, Kristof Z, Vagi P, Zatyko L (2006) Production of doubled haploid breeding lines in case of paprika, spice paprika, eggplant, cucumber, zucchini and onion. Acta Hortic 725:845–854
González-Melendi P, Testillano PS, Ahmadian P, Fadón B, Vicente O, Risueño MC (1995) In situ characterization of the late vacuolate microspore as a convenient stage to induce embryogenesis in Capsicum. Protoplasma 187:60–71
Isouard G, Raquin C, Demarly Y (1979) Obtention de plantes haploides et diploides par culture in vitro d’anthères dáubergine (Solanum melongena L.). C R Acad Sci Paris 288:987–989
Kim M, Kim J, Yoon M, Choi D-I, Lee K-M (2004) Origin of multicellular pollen and pollen embryos in cultured anthers of pepper (Capsicum annuum). Plant Cell Tiss Organ Cult 77:63–72
Kim M, Jang I-C, Kim J-A, Park E-J, Yoon M, Lee Y (2008) Embryogenesis and plant regeneration of hot pepper (Capsicum annuum L.) through isolated microspore culture. Plant Cell Rep 27:425–434
Lichter R (1982) Induction of haploid plants from isolated pollen of Brassica napus. Z Pflanzenphysiol 105:427–434
Matsubara S, Hu KL, Murakami K (1992) Embryoid and callus formation from pollen grains of eggplant and pepper by anther culture. J Jpn Soc Hortic Sci 61:69–77
Miyoshi K (1996) Callus induction and plantlet formation through culture of isolated microspores of eggplant (Solanum melongena L). Plant Cell Rep 15:391–395
Muñoz-Falcón JE, Prohens J, Vilanova S, Nuez F (2008a) Characterization, diversity, and relationships of the Spanish striped (Listada) eggplants: a model for the enhancement and protection of local heirlooms. Euphytica 164:405–419
Muñoz-Falcón JE, Prohens J, Vilanova S, Ribas F, Castro A, Nuez F (2008b) Distinguishing a protected geographical indication vegetable (Almagro eggplant) from closely related materials with selected morphological traits and molecular markers. J Sci Food Agric 89:320–328
Muñoz-Falcón JE, Prohens J, Vilanova S, Nuez F (2009) Diversity in commercial varieties and landraces of black eggplants and implications for broadening the breeders gene pool. Ann Appl Biol 154:453–465
Palmer CE, Keller WA, Kasha KJ (2005) Haploids in crop improvement II. Springer, Berlin
Prohens J, Rodríguez-Burruezo A, Raigón MD, Nuez F (2007) Total phenolics concentration and browning susceptibility in a collection of different varietal types and hybrids of eggplant: implications for breeding for higher nutritional quality and reduced browning. J Am Soc Hortic Sci 132:638–646
Quagliotti L (1979) Floral biology of Capsicum and Solanum melongena. In: Hawkes JG, Lester RN, Skelding AD (eds) The biology and taxonomy of the Solanaceae. Academic Press for the Linnean Society, London, pp 399–419
Raina SK, Iyer RD (1973) Differentiation of diploid plants from pollen callus in anther cultures of Solanum melongena L. Z Pflanzenzucht 70:275–280
Rotino GL (1996) Haploidy in eggplant. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants. Kluwer Academic Publishers, Dordrecht, pp 115–141
Rotino GL, Falavigna A, Restaino F (1987) Production of anther-derived plantlets of eggplant. Capsicum Newsl 6:89–90
Rotino GL, Restaino F, Gjomarkaj M, Massimo M, Falavigna A, Schiavi M, Vicini E (1991) Evaluation of genetic variability in embryogenetic and androgenetic lines of eggplant. Acta Hortic 300:357–362
Rudolf K, Bohanec B, Hansen M (1999) Microspore culture of white cabbage, Brassica oleracea var capitata L.: Genetic improvement of non-responsive cultivars and effect of genome doubling agents. Plant Breed 118:237–241
Rylski I, Nothmann J, Arcan L (1984) Differential fertility in short-styled eggplant flowers. Sci Hort 22:39–46
Salas P, Prohens J, Seguí-Simarro JM (2011) Evaluation of androgenic competence through anther culture in common eggplant and related species. Euphytica 182:261–274
Sanchez-Mata MC, Yokoyama WE, Hong YJ, Prohens J (2010) α-solasonine and α-solamargine contents of gboma (Solanum macrocarpon L.) and scarlet (Solanum aethiopicum L.) eggplants. J Agric Food Chem 58:5502–5508
Sanguineti MC, Tuberosa R, Conti S (1990) Field evaluation of androgenetic lines of eggplant. Acta Hortic 280:177–182
Seguí-Simarro JM (2010) Androgenesis revisited. Bot Rev 76:377–404
Seguí-Simarro JM, Nuez F (2005) Meiotic metaphase I to telophase II is the most responsive stage of microspore development for induction of androgenesis in tomato (Solanum lycopersicum). Acta Physiol Plant 27:675–685
Seguí-Simarro JM, Nuez F (2008) How microspores transform into haploid embryos: changes associated with embryogenesis induction and microspore-derived embryogenesis. Physiol Plant 134:1–12
Seguí-Simarro JM, Bárány I, Suárez R, Fadón B, Testillano PS, Risueño MC (2006) Nuclear bodies domain changes with microspore reprogramming to embryogenesis. Eur J Histochem 50:35–44
Seguí-Simarro JM, Corral-Martínez P, Parra-Vega V, González-García B (2011) Androgenesis in recalcitrant solanaceous crops. Plant Cell Rep 30:765–778
Sekara A, Bieniasz M (2008) Pollination fertilization and fruit formation in eggplant (Solanum melongena L.). Acta Agrobotanica 61:107–113
Shariatpanahi ME, Bal U, Heberle-Bors E, Touraev A (2006) Stresses applied for the re-programming of plant microspores towards in vitro embryogenesis. Physiol Plant 127:519–534
Supena EDJ, Muswita W, Suharsono S, Custers JBM (2006a) Evaluation of crucial factors for implementing shed-microspore culture of Indonesian hot pepper (Capsicum annuum L.) cultivars. Sci Hort 107:226–232
Supena EDJ, Suharsono S, Jacobsen E, Custers JBM (2006b) Successful development of a shed-microspore culture protocol for doubled haploid production in Indonesian hot pepper (Capsicum annuum L.). Plant Cell Rep 25:1–10
Touraev A, Pfosser M, Heberle-Bors E (2001) The microspore: a haploid multipurpose cell. Adv Bot Res 35:53–109
Touraev A, Forster BP, Jain SM (2009) Advances in haploid production in higher plants. Springer, New York
Tuberosa R, Sanghineti MC, Conti S (1987) Anther culture of eggplant Solanum melongena L lines and hybrids. Genética Agrária 41:267–274
Whalen MD, Costich DE (1986) Andromonoecy in Solanum. In: D’Arcy W,G (ed) Solanaceae Biology and systematics. Columbia University Press, New York, pp 284–302
Acknowledgments
We want to acknowledge Drs. Begoña Renau and Antonio Serrano, and Mrs Nuria Palacios for their excellent technical work, as well as the staff of the COMAV greenhouses for their valuable help. We also thank the anonymous reviewers for their excellent criticisms and the ideas they contributed. This work was supported by grant AGL2010-17895 from Spanish MICINN to JMSS.
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Salas, P., Rivas-Sendra, A., Prohens, J. et al. Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures. Euphytica 184, 235–250 (2012). https://doi.org/10.1007/s10681-011-0569-9
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DOI: https://doi.org/10.1007/s10681-011-0569-9