Interspecific hybridization is a useful technique to introduce characteristics from wild species into crops. Garden asparagus (Asparagus officinalis, 2n = 2x = 20) is an economically important vegetable that is native to the Mediterranean region but widely cultivated in many countries. The genus Asparagus is comprised of over 100 species. Asparagus kiusianus (2n = 2x = 20) is a wild asparagus species endemic to Japan. This species occurs on the coast and is likely to be a salt-resistant species. Although the geographic distribution of these two species is not close, molecular phylogenetic analysis demonstrated that these two species are closely related. In this study, a reciprocal cross between A. officinalis and A. kiusianus was carried out by hand pollination, and progeny were obtained from both crossings. These progeny exhibited a morphologically intermediate phenotype in terms of flower shape, and restriction fragment length polymorphism analysis confirmed that these were indeed interspecific hybrids. The interspecific hybrids were fertile, and backcross progeny with garden asparagus was also generated. These interspecific hybrids are expected to supply novel traits to garden asparagus.
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Alexander MP (1969) Diferential staining of aborted and nonaborted pollen. Stain Technol 44:117–122
Bailey LH (1944) Asparagus. In: Bailey LH (ed) The standard cyclopedia of horticulture. The Macmillan Company, New York, pp 406–411
Chittenden FJ (1956) Asparagus. In: Chittenden FJ (ed) Dictionary of gardening. Clarendon Press, Oxford, pp 193–196
Clifford HT, Conran JG (1987) Asparagaceae. In: George AS (ed) Flora of Australia. Australian Government Publishing Service, Canberra, pp 159–164
Dahlgren RMT, Clifford HT, Yeo PF (1985) Asparagaceae. In: Dahlgren RMT, Clifford HT, Yeo PF (eds) The families of the monocotyledons. Springer-Verlag, Berlin, pp 140–142
Falavigna A, Alberti P, Casali PE, Toppino L, Huaisong W, Mennella G (2008) Interspecific hybridization for asparagus breeding in Italy. Acta Hort 776:291–297
Flory WS Jr (1932) Genetic and cytological investigations on Asparagus officinalis L. Genetics 17:432–467
Fukuda T, Song IJ, Nakamura T, Nakada M, Kanno A, Kameya T, Yamaji H, Terabayashi A, Honda H, Hirai A (1990) A simple and efficient method for identification of hybrids using nonradioactive rDNA as probe. Jpn J Breed 40:339–348
Fukuda T, Ashizawa H, Suzuki R, Nakamura T, Ochiai T, Kanno A, Kameya T, Yokoyama J (2005) Molecular phylogeny of the genus Asparagus (Asparagaceae) inferred from plastid petB intron and petD-rpoA intergenic spacer sequences. Plant Species Biol 20:121–132
Honda H, Hirai A (1990) A simple and efficient method for identification of hybrids using nonradioactive rDNA probe. Jap J Breed 40:339–348
Ito T, Ochiai T, Ashizawa H, Shimodate T, Sonoda T, Fukuda T, Yokoyama J, Kameya T, Kanno A (2007) Production and analysis of reciprocal hybrids between Asparagus officinalis L. and A. schoberioides Kunth. Genet Resour Crop Evol 54:1063–1071
Kanno A, Kameya T (1999) Cloning and variation of ribosomal DNA from Asparagus officinalis L. Acta Hort 479:365–372
Kar DK, Sen S (1985) Chromosome characteristics of Asparagus-Sapogenin yielding plant. Cytologia 50:147–155
Komarov VL (1968) Asparagus L. In: Komarov VL (ed) Flora of the USSR vol IV. Israel Program for Scientific Translations, Jerusalem, pp 325–339
Kubituki K, Rudall PJ (1998) Asparagaceae. In: Kubituki K (ed) The families and genera of vascular plants, vol 3. Springer-Verlag, Berlin, pp 125–128
Kunitake H, Nakashima T, Mori K, Tanaka M, Saito A, Mii M (1996) Production of interspecific somatic hybrid plants between Asparagus officinalis and A. macowanii through electrofusion. Plant Sci 116:213–222
Lee YO, Kanno A, Kameya T (1997) Phylogenetic relationships in the genus Asparagus based on the restriction enzyme analysis of the chloroplast DNA. Breed Sci 47:375–378
Marcellán ON, Camadro EL (1996) Self- and cross-incompatibility in Asparagus officinalis and Asparagus densiflorus cv. Sprengeri. Can J Bot 74:1621–1625
Marcellán ON, Camadro EL (1999) Formation and development of embryo and endosperm in intra- and inter-specific cross of Asparagus officinalis and A. densiflorus cv. Sprengeri. Sci Hort 81:1–11
Ochiai T, Sonoda T, Kanno A, Kameya T (2002) Interspecific hybrids between Asparagus schoberioides Kunth and A. officinalis L. Acta Hort 589:225–229
Ohwi J (1965) Asparagus L. In: Meyer FG, Walker EH (eds) Flora of Japan. Smithsonian Institution, Washington DC, p 300
Stajner N, Bohance B, Jakse M (2002a) In vitro propagation of Asparagus maritimus—a rare Mediterranean salt-resistant species. Plant Cell Tissue Organ Cult 70:269–274
Stajner N, Bohance B, Javornik B (2002b) Genetic variability of economically important Asparagus species as revealed by genome size analysis and rDNA ITS polymorphisms. Plant Sci 162:931–937
Valdes B (1964) Asparagus L. In: Tutin TG, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM, Webb DA (eds) Flora Europaea. Cambridge University Press, Cambridge, pp 71–73
We thank all our colleagues for providing assistance and advice. This study was supported in part by a grant to the Itoh Science Foundation, a Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan and by a Research Promotion for the Graduate School in Life Sciences, Tohoku University.
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Ito, T., Konno, I., Kubota, S. et al. Production and characterization of interspecific hybrids between Asparagus kiusianus Makino and A. officinalis L.. Euphytica 182, 285 (2011). https://doi.org/10.1007/s10681-011-0508-9
- Flow cytometry
- Morphological comparison
- Restriction fragment length polymorphism (RFLP)
- Interspecific hybrid
- Pollen fertility