Molecular cytogenetic characterization and SSR marker analysis of a leaf rust resistant wheat line carrying a 6G(6B) substitution from Triticum timopheevii (Zhuk.)
A disease (powdery mildew, leaf rust) resistant line was selected from the progenies of a Triticum aestivum × Triticum timopheevii amphiploid produced at Martonvásár. This line was previously identified with C-banding as a 6G(6B) substitution. In order to detect the 6G chromosome in a wheat background, fluorescence in situ hybridization (FISH) and microsatellite marker analysis were used. Ten microsatellite markers of the 43 tested generated PCR products that were polymorphic between chromosomes 6B and 6G, and four showed length-polymorphism. The FISH hybridization pattern of 6G from T. timopheevii was identified using a combination of four repetitive DNA probes (Afa-family, pSc119.2, pTa71, (GAA)7). Genomic in situ hybridization (GISH) technique, capable of labelling the At and G genomes separately, was used on the same slides to differentiate the At and G genomes in T. timopheevii. The At and G genomes of T. timopheevii were grouped on the basis of the GISH patterns and a cyclic intergenomic translocation involving 6At-1G-4G was detected in T. timopheevii accession TRI667. The presence of 6G in the substitution line was demonstrated using FISH with the four repetitive DNA probes. Chromosome 6G was clearly identified and its FISH pattern was different from that of 6B in the parental wheat cultivar Fleischmann-481. According to field tests, the 6G(6B) substitution line has resistance to leaf rust.
KeywordsTriticum aestivum T. timopheevii Leaf rust resistance Fluorescence in situ hybridization Microsatellite marker
Thanks are due to Dr. István Molnár for his help with GISH examinations and to Mrs. B. Harasztos for revising the manuscript linguistically. This study was supported by grants from the Hungarian Ministry of Education (OM188/2007), the European Union (EU-FP7-REGPOT-2007-1, AGRISAFE No.203288) and the National Scientific Research Fund (K 75381).
- Badaeva ED, Badaev NS, Enno TM, Zeller FJ, Peusha HO (1995) Chromosome substitution in progeny of hybrids Triticum aestivum × Triticum timopheevii, resistant to brown rust and powdery mildew. Russ J Genet 31:75–77Google Scholar
- Belea A (1961) Cercetari privind amfidiploidul Triticum aestivotimopheevi in F2 si in generatiieleurmatoare. Probl Agric 8:1–21Google Scholar
- Enno T, Peusha H, Timofeyeva L, Tohver M, Yakobson I, Priilinn O (1998) Identification of chromosomal translocations in common wheat, derivative of Triticum timopheevii. Acta Agron Hung 46:209–216Google Scholar
- McIntosh RA (1983) Genetic and cytogenetic studies involving Lr18 for resistance to Puccinia recondita. In: Sakamoto S (ed) Proceedings of the sixth international wheat genetics symposium, Faculty of Agriculture, Kyoto University, Japan, pp 777–783Google Scholar
- McIntosh RA (1988) Catalogue of gene symbols for wheat. In: Miller TE, Koebner RMD (eds) Proceedings of the 7th international wheat genetics symposium, Cambridge, UK, pp 1225–1320Google Scholar
- McIntosh RA, Gyarfas J (1971) Triticum timopheevii as a source of resistance to wheat stem rust. Z Pflanzenzücht 66:240–248Google Scholar
- Molnár-Láng M, Kőszegi B, Linc G, Sutka J (1996) Detection of wheat (Triticum aestivum L./Triticum timopheevii Zhuk. addition and substitution and wheat/rye translocation by C-banding and in situ hybridization. [Búza (Triticum aestivum L.)/Triticum timopheevii Zhuk. addíció, szubsztitució és búza/rozs transzlokáció kimutatása C-sávozással és in situ hibridizációval]. Növénytermelés 45:237–245Google Scholar
- Peusha HO, Stephan U, Hsam SLK, Felsenstein FG, Enno TM, Zeller FJ (1995) Identification of genes for resistance to powdery mildew in common wheat (Triticum aestivum L.) IV. Breeding lines derived from wide crosses of russian cultivars with species T. timopheevii Zhuk., T. militinae Zhuk., et Migush., T. dicoccum (Schrank.) Schuebl., Aegilops speltoides Taush. Russ J Genet 31:1–7Google Scholar
- Peusha HO, Enno TM, Priilinn O (1996) Genetic analysis of disease resistance in wheat hybrids, derivatives of Triticum timopheevii and Triticum militinae. Acta Agron Hung 44:237–244Google Scholar
- Rayburn AL, Gill BS (1985) Use of biotin-labeled probes to map specific DNA sequences on wheat chromosomes. J Hered 76:78–81Google Scholar
- Schwarzacher T, Leitch AR, Bennett MD, Heslop-Harrison JS (1989) In situ localization of parental genomes in a wild hybrid. Ann Bot (Lond) 64:315–324Google Scholar
- Yang ZJ, Ren ZL (1997) Expression of gene Pm8 for resistance to powdery mildew in wheat for Sichuan. J Sichuan Agric Univ 15:452–456Google Scholar