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Reciprocal differences in DNA sequence and methylation status of the pollen DNA between F1 hybrids of Solanum tuberosum × S. demissum

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Abstract

Reciprocal differences, mostly caused by cytoplasmic effects, are frequently observed in interspecific hybrids. Previously, we found that crosses onto Solanum demissum were much successful with the pollen of interspecific hybrids between S. tuberosum as female and S. demissum as male (TD hybrids) than the reciprocal ones (DT hybrids). To elucidate this reciprocally different crossability, we analyzed the pollen DNA of TD and DT using methylation-sensitive amplified polymorphism (MSAP) analysis. Using 126 primer combinations, MSAP analysis revealed 57 different bands between bulked pollen DNA samples of TD and DT. Individual examination of 16 TD and 9 DT plants disclosed eight bands uniformly different between TD and DT. Their sequencing results revealed two pairs of bands to be identical to each other, resulting in six distinct sequences. As expected, one band shared high homology with chloroplast DNA, and another one with mitochondrial DNA. However, one band that was apparently different at DNA sequence level and maternally transmitted from S. demissum, showed no homology with any known sequence. The remaining three bands were of DNA methylation level differences with no or uncertain homology to known sequences. To our knowledge, this is the first report detecting reciprocal differences in DNA sequence or DNA methylation other than those in cytoplasmic DNA.

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Acknowledgments

We thank the US Potato Genebank (NRSP-6), Sturgeon Bay, Wisconsin, for providing seeds of S. demissum. This study was carried out at the Food Resources Education and Research Center, Kobe University, and supported partly by Grant-in-Aid for Scientific Research (C) (18580005), MEXT, and Calbee Potato, Inc.

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  1. Graduate School of Agricultural Science, Kobe University, Nada, Kobe, Hyogo, 657-8501, Japan

    Rena Sanetomo

  2. Calbee Potato Inc., East Memuro, Memuro, Hokkaido, 082-0006, Japan

    Rena Sanetomo

  3. National Agricultural Research Center for Hokkaido Region, Shinsei, Memuro, Hokkaido, 082-0081, Japan

    Kazuyoshi Hosaka

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  1. Rena Sanetomo
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Correspondence to Kazuyoshi Hosaka.

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Sanetomo, R., Hosaka, K. Reciprocal differences in DNA sequence and methylation status of the pollen DNA between F1 hybrids of Solanum tuberosum × S. demissum . Euphytica 182, 219–229 (2011). https://doi.org/10.1007/s10681-011-0444-8

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