Abstract
Four cotton species (genus Gossypium) produce spinable fiber. The two diploid species of Asiatic origin, Gossypium arboreum and G. herbaceum, have been largely replaced by G. hirsutum. However, these diploid species are potentially a rich source of genes for the improvement of G. hrisutum, particularly in terms of providing resistance against biotic and abiotic stresses. As a first step towards understanding the mechanisms of resistance in cotton, we designed 24 non-degenerate primers based on resistance gene analogs (RGAs) cloned from G. hirsutum for screening a number of cotton species with the A and D genomes. Most of these RGAs are conserved on the A genome (G. arboreum), suggesting a bias towards this genome. The amplified RGAs from G. arboreum were cloned and their nucleotide and amino acid sequences compared with RGA sequences available in public databases. The majority of the RGAs identified were homologous to those isolated from G. hirsutum and G. barbadense, but their diversity was greater than expected at both the nucleotide and amino acid levels. These RGAs provide useful tools for the identification of full-length resistance genes from bacterial artificial chromosome and cDNA libraries.
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Acknowledgments
The authors are grateful to Dr. Rob W. Briddon for his critical reading of the manuscript. MTA was supported by PhD fellowships from the Higher Education Commission (HEC), Government of Pakistan. The authors are grateful for the support of NIBGE in conducting this study. Funding was provided by the Ministry of Science and Technology (MoST) and the Ministry of Food, Agriculture (MINFA), Government of Pakistan.
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Azhar, M.T., Amin, I., Bashir, A. et al. Characterization of resistance gene analogs from Gossypium arboreum and their evolutionary relationships with homologs from tetraploid cottons. Euphytica 178, 351–362 (2011). https://doi.org/10.1007/s10681-010-0310-0
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DOI: https://doi.org/10.1007/s10681-010-0310-0