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Genetic variability of seed-quality traits in gamma-induced mutants of sunflower (Helianthus annuus L.) under water-stressed condition

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Abstract

Sunflower is one of the major annual world crops grown for edible oil and its meal is a potential source of protein for human consumption. It contains tocopherol that decreases potential risk of chronic diseases in human. The objectives of the current research are to assess the genetic variability and to identify AFLP markers and candidate genes associated with seed-quality traits under well-irrigated and water-stressed conditions in gamma-induced mutants of sunflower. Two mutant lines, M8-826-2-1 and M8-39-2-1, with significant increased level of oleic acid were identified that can be used in breeding programs for quality increase high oxidative stability and heart-healthy properties. The significant increased level of tocopherol in mutant lines, M8-862-1N1 and M8-641-2-1, is justified by observed polymorphism for tocopherol pathway-related gene; MCT. The most important marker for total tocopherol content is E33M50_16 which explains 33.9% of phenotypic variance. One of the most important candidate genes involving fatty acid biosynthesis, FAD2 (FAD2-1), is linked to oleic and linoleic acids content and explained more than 53% of phenotypic variance. Common markers associated with different seed-quality traits in well-irrigated and water-stressed conditions could be used for marker-assisted selection (MAS) in both conditions. Other markers, which are specific for one condition whereas linked to different traits or specific for a trait, could be useful for a given water treatment.

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Acknowledgments

This research was supported by Gundishapur project No. 12267RD. The authors wish to thank the French and Iranian governments for providing fellowship for Ph.D. program of the first author.

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Authors and Affiliations

  1. Laboratoire des Interactions Plantes Micro-organismes, UMR CNRS-INRA 2594/441, Chemin de Borde-Rouge Auzeville, BP 52627, 31326, Castanet Tolosan, France

    P. Haddadi

  2. Agronomy and Plant Breeding Department, Faculty of Agriculture, University of Tehran, Karaj, Iran

    P. Haddadi, B. Yazdi-samadi & M. R. Naghavi

  3. Laboratoire de Symbiose et Pathologie des Plantes, SP2, IFR 40, INP-ENSAT, 18 Chemin de Borde Rouge, BP 32607, 31326, Castanet Tolosan, France

    P. Haddadi & A. Sarrafi

  4. UMR 1054 INRA/EIP - Laboratoire d’Agrophysiologie, Ecole d’Ingénieurs de Purpan. 75, Voie du TOEC, BP 57611, 31076, Toulouse Cedex 3, France

    M. Berger & A. Calmon

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  1. P. Haddadi
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  2. B. Yazdi-samadi
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Correspondence to A. Sarrafi.

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Haddadi, P., Yazdi-samadi, B., Berger, M. et al. Genetic variability of seed-quality traits in gamma-induced mutants of sunflower (Helianthus annuus L.) under water-stressed condition. Euphytica 178, 247–259 (2011). https://doi.org/10.1007/s10681-010-0309-6

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