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Characterization of two new Puccinia graminis f. sp. tritici races within the Ug99 lineage in South Africa

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Abstract

Two new races of the wheat (Triticum aestivum L.) stem rust pathogen, representing the fifth and sixth variants described within the Ug99 lineage, were detected in South Africa. Races TTKSP and PTKST (North American notation) were detected in 2007 and 2009, respectively. Except for Sr24 virulence, race TTKSP is phenotypically identical to TTKSF, a commonly detected race of Puccinia graminis f. sp. tritici (Pgt) in South Africa. PTKST is similar to TTKSP except that it produces a lower infection type on the Sr21 differential and has virulence for Sr31. Simple sequence repeat (SSR) analysis confirmed the genetic relationship amongst TTKSF, TTKSP, PTKST and TTKSK (Ug99). TTKSK, PTKST and TTKSF grouped together with 99% similarity, while sharing 88% genetic resemblance with TTKSP. These four races in turn shared only 31% similarity with other South African races. It is proposed that both TTKSP and PTKST represent exotic introductions of Pgt to South Africa.

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References

  • Bandelt H-J, Forster P, Röhl A (1999) Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol 16:37–48

    PubMed  CAS  Google Scholar 

  • Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491

    PubMed  CAS  Google Scholar 

  • Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50

    PubMed  CAS  Google Scholar 

  • Hartl DL, Clark AG (1997) Principles of population genetics, 3rd edn. Sinauer Associates, Sunderland, MA

    Google Scholar 

  • Jaccard P (1908) Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270

    Google Scholar 

  • Jin Y, Singh RP, Ward RW, Wanyera R, Kinyua M, Njau P, Fetch T, Pretorius ZA, Yahyaoui A (2007) Characterization of seedling infection types and adult plant infection responses of monogenic Sr gene lines to race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 91:1096–1099

    Article  Google Scholar 

  • Jin Y, Szabo LJ, Pretorius ZA (2008a) Virulence variation within the Ug99 lineage. In: Appels R, Eastwood R, Lagudah E, Langridge P, Mackay M, McIntyre L, Sharp P (eds) Proceedings of 11th International Wheat Genetics Symposium, Brisbane, Australia. Sydney University Press eScholarship Repository http://ses.library.usyd.edu.au/ paper O02. Accessed 19 April 2010

  • Jin Y, Szabo LJ, Pretorius ZA, Singh RP, Ward R, Fetch T (2008b) Detection of virulence to resistance gene Sr24 within race TTKS of Puccinia graminis f. sp. tritici. Plant Dis 92:923–926

    Article  Google Scholar 

  • Jin Y, Szabo LJ, Rouse MN, Fetch T, Pretorius ZA, Wanyera R, Njau P (2009) Detection of virulence to resistance gene Sr36 within the TTKS race lineage of Puccinia graminis f. sp. tritici. Plant Dis 93:367–370

    Article  CAS  Google Scholar 

  • Le Roux J, Rijkenberg FHJ (1987) Pathotypes of Puccinia graminis f. sp. tritici with increased virulence for Sr24. Plant Dis 71:1115–1119

    Article  Google Scholar 

  • MacKenzie D (2008) Killer wheat fungus threatens starvation of millions. New Sci 2647:14–15

    Google Scholar 

  • Mantel NA (1967) The detection of disease clustering and a generalised regression approach. Cancer Res 27:209–220

    PubMed  CAS  Google Scholar 

  • McIntosh RA, Wellings CR, Park RF (1995) Wheat rusts: an atlas of resistance genes. Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Park RF, Bariana HS (2008) Status of Ug99 resistance in current Australian wheat cultivars and breeding materials. In: Appels R, Eastwood R, Lagudah E, Langridge P, Mackay M, McIntyre L, Sharp P (eds) Proceedings 11th International Wheat Genetics Symposium, Brisbane, Australia. Sydney University Press eScholarship Repository http://ses.library.usyd.edu.au/ paper O07. Accessed 19 April 2010

  • Pretorius ZA, Singh RP, Wagoire WW, Payne TS (2000) Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f. sp. tritici in Uganda. Plant Dis 84:203

    Article  Google Scholar 

  • Pretorius ZA, Pakendorf KW, Marais GF, Prins R, Komen JS (2007) Challenges for sustainable cereal rust control in South Africa. Aust J Agric Res 58:593–601

    Article  Google Scholar 

  • Pretorius ZA, Bender CM, Visser B, Terefe T (2010) First report of a Puccinia graminis f. sp. tritici race virulent to the Sr24 and Sr31 wheat stem rust resistance genes in South Africa. Plant Dis 94:784

    Article  Google Scholar 

  • Rohlf FJ (1998) NTSYS-pc: numerical taxonomy and multivariate analysis system. Version 2.02. Exeter Software, Setauket, New York

    Google Scholar 

  • Rowell JB, Romig RW (1966) Detection of urediospores of wheat rusts in spring rains. Phytopathology 56:807–811

    Google Scholar 

  • Saari EE, Prescott JM (1985) World distribution in relation to economic loss. In: Roelfs AP, Bushnell WR (eds) The cereal rusts volume II: diseases distribution epidemiology and control. Academic Press, Orlando, pp 259–289

    Google Scholar 

  • Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location and population dynamics. Proc Nat Acad Sci USA 81:8014–8018

    Article  PubMed  CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniates T (1989) Molecular cloning, a laboratory manual, 2nd edn. Cold Spring Harbour Laboratory Press, New York

    Google Scholar 

  • Singh RP, McIntosh RA (1987) Genetics of resistance to Puccinia graminis tritici in “Chris” and “W3746” wheats. Theor Appl Genet 73:846–855

    Article  Google Scholar 

  • Singh RP, Hodson DP, Jin Y, Huerta-Espino J, Kinyua MG, Wanyera R, Njau P, Ward RW (2006) Current status, likely migration and strategies to mitigate the threat to wheat production from race Ug99 (TTKS) of stem rust pathogen. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources 1:1–13

    CAS  Google Scholar 

  • Singh RP, Hodson DP, Huerta-Espino J, Jin Y, Njau P, Wanyera R, Herrera-Foessel SA, Ward RW (2008) Will stem rust destroy the world’s wheat crop? Adv Agron 98:271–309

    Article  CAS  Google Scholar 

  • Sokal RR, Michener CD (1958) A statistical method for evaluating systematic relationships. Univ Kans Sci Bull 38:1409–1438

    Google Scholar 

  • Stakman EC, Stewart DM, Loegering WQ (1962) Identification of physiologic races of Puccinia graminis var. tritici. USDA-ARC E167

  • Szabo LJ (2007) Development of simple sequence repeat markers for the plant pathogenic rust fungus, Puccinia graminis. Mol Ecol Notes 7:92–94

    Article  CAS  Google Scholar 

  • Terefe TG, Pretorius ZA, Paul I, Mebalo J, Meyer L, Naicker K (2010) Occurrence and pathogenicity of Puccinia graminis f. sp. tritici on wheat in South Africa during 2007 and 2008. S Afr J Plant Soil 27:163–167

    Google Scholar 

  • Visser B, Herselman L, Pretorius ZA (2009) Genetic comparison of Ug99 with selected South African races of P. graminis f. sp. tritici. Mol Plant Pathol 10:213–222

    Article  PubMed  CAS  Google Scholar 

  • Wanyera R, Kinyua MG, Jin Y, Singh RP (2006) The spread of stem rust caused by Puccinia graminis f. sp. tritici with virulence on Sr31 in Eastern Africa. Plant Dis 90:113

    Article  Google Scholar 

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Acknowledgments

The Winter Cereal Trust is acknowledged for funding this project.

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Authors and Affiliations

  1. Department of Plant Sciences, University of the Free State, P.O. Box 339, Bloemfontein, 9300, South Africa

    Botma Visser, Liezel Herselman, Cornelia M. Bender & Zacharias A. Pretorius

  2. Plant Breeding Institute Cobbitty, The University of Sydney, Private Mail Bag 11, Camden, NSW, 2570, Australia

    Robert F. Park & Haydar Karaoglu

Authors
  1. Botma Visser
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  2. Liezel Herselman
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  3. Robert F. Park
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  4. Haydar Karaoglu
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  5. Cornelia M. Bender
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  6. Zacharias A. Pretorius
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Correspondence to Botma Visser.

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Visser, B., Herselman, L., Park, R.F. et al. Characterization of two new Puccinia graminis f. sp. tritici races within the Ug99 lineage in South Africa. Euphytica 179, 119–127 (2011). https://doi.org/10.1007/s10681-010-0269-x

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  • DOI: https://doi.org/10.1007/s10681-010-0269-x

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