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Euphytica

, Volume 177, Issue 3, pp 383–392 | Cite as

Expression of Bruguiera gymnorhiza BgARP1 enhances salt tolerance in transgenic Arabidopsis plants

  • Masashi Miyama
  • Yuichi TadaEmail author
Article

Abstract

We have previously reported that expression of salt-responsive genes, including Bruguiera gymnorhiza ankyrin repeat protein 1 (BgARP1), enhances salt tolerance in both Agrobacterium tumefaciens and Arabidopsis. In this report, we further characterized BgARP1-expressing Arabidopsis to elucidate the role of BgARP1 in salt tolerance. BgARP1-expressing plants exhibited more vigorous growth than wild-type plants on MS plates containing 125–175 mM NaCl. Real-time PCR analysis showed enhanced induction of osmotin34 in the 2-week-old transformants under 125 mM NaCl. It was also showed that induction of typical salt-responsive genes, including RD29A, RD29B, and RD22, was blunted and delayed in the 4-week-old transformants during 24 h after 200 mM NaCl treatment. Ion content analysis showed that transgenic plants contained more K+, Ca2+, and NO3 , and less NH4 +, than wild-type plants grown in 200 mM NaCl. Our results suggest that BgARP1-expressing plants may reduce salt stress by up-regulating osmotin34 gene expression and maintaining K+ homeostasis and regulating Ca2+ content. These results indicate that BgARP1 is functional on a heterogeneous background.

Keywords

Bruguiera gymnorhiza Salt tolerance BgARP1 Ankyrin repeat protein Transgenic Arabidopsis Mangrove 

Notes

Acknowledgment

This work was partially supported by Grant-in Aid for Scientific Research (C) for YT (20580008) and Grant-in Aid for Scientific Research (B) for YT (21380002).

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  1. 1.School of Bioscience and BiotechnologyTokyo University of TechnologyHachiojiJapan
  2. 2.Research Center of Advanced BionicsAdvanced Industrial Science and TechnologyHachiojiJapan

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