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Vitamin C, flower color and ploidy variation of hybrids from a ploidy-unbalanced Actinidia interspecific cross and SSR characterization

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Abstract

Seedlings derived from an Actinidia interspecific cross between the hexaploid Actinidia chinensis var. deliciosa ‘Jinkui’ and the diploid male A. eriantha × A. chinensis var. chinensis ‘Chaohong’ hybrid were analyzed using flow cytometry, SSR markers and phenotypic observations. The results show that the leaf vitamin C content of this hybrid population has a mid-parent heterosis. Separation of flower color in the progeny was also observed, progeny with red flowers lighter than ‘Chaohong’, white flowers as in ‘Jinkui’ and intermediate types with a red base to the petals and white margins were all present. Flow cytometry analysis confirmed that most of the progeny were tetraploids, and molecular marker data showed that most of these tetraploid progeny had three alleles from the hexaploid parent and one allele from the diploid parent. UPGMA analysis based on the SSR markers showed that the diploid parent was completely separated from the hexaploid parent and all the progeny.

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Acknowledgements

The authors gratefully thank Dr. A. Ross Ferguson from Plant & Food Research, New Zealand, Dr. Raffaele Testolin from University of Udine, and Dr. Norman C. Ellstrand from University of California, Riverside for their critical review of the manuscript and helpful suggestions, Zongzhou Xie from Huazhong Agricultural University for helping in FCM analysis. This research was partially supported by the National Natural Science Foundation of China (Grant no. 30671433), the Knowledge Innovation Program (KSCX2-YW-N-061 and KSCX2-YW-Z-052) of the Chinese Academy of Sciences, and the Directional Project of Chinese Academy of Sciences for Zuozhou Li.

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  1. Key Laboratory of Plant Germplasm Enhancement and Speciality Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China

    Lei Zhang, Zuozhou Li, Yanchang Wang, Zhengwang Jiang, Shengmei Wang & Hongwen Huang

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Lei Zhang

  3. South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China

    Hongwen Huang

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  1. Lei Zhang
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Zhang, L., Li, Z., Wang, Y. et al. Vitamin C, flower color and ploidy variation of hybrids from a ploidy-unbalanced Actinidia interspecific cross and SSR characterization. Euphytica 175, 133–143 (2010). https://doi.org/10.1007/s10681-010-0194-z

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