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Selection of U and M genome-specific wheat SSR markers using wheat–Aegilops biuncialis and wheat–Ae. geniculata addition lines

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Abstract

We selected wheat SSR markers specific to the U and M genomes of Aegilops species. A total of 108 wheat SSR markers were successfully tested on Ae. biuncialis (2n = 4x = 28, UbUbMbMb), on five wheat–Ae. biuncialis addition lines (2Mb, 3Mb, 7Mb, 3Ub and 5Ub) and on a wheat–Ae. geniculata (1Ug, 2Ug, 3Ug, 4Ug, 5Ug, 7Ug, 1Mg, 2Mg, 4Mg, 5Mg, 6Mg and 7Mg) addition series. Among the markers, 86 (79.6%) were amplified in the Ae. biuncialis genome. Compared with wheat, polymorphic bands of various lengths were detected on Ae. biuncialis for 35 (32.4%) of the wheat microsatellite markers. Three of these (8.6%) exhibited specific PCR products on wheat–Ae. biuncialis or wheat–Ae. geniculata addition lines. The primers GWM44 and GDM61 gave specific PCR products on the 2Mb and 3Mb wheat–Ae. biuncialis addition lines, but not on the 2Mg addition line of Ae. geniculata. A specific band was observed on the 7Ug wheat–Ae. geniculata addition line using the BARC184 primer. These three markers specific to the U and M genomes are helpful for the identification of 2Mb, 3Mb and 7Ug chromosome introgressions into wheat.

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Acknowledgements

The technical assistance of Mrs. J. Bucsi, Mrs. E. Türkösi and Mrs. I. Keserű is gratefully acknowledged. This work was financially supported by the Hungarian National Scientific Research Fund, No. PD75450 and by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences. The Syngene G Box gel documentation system used in this research was purchased with funds from the AGRISAFE Project (No. 203288 EU-FP7-REGPOT 2007-1). We thank Dr. B. Friebe for supplying the wheat–Ae. geniculata addition lines and express our gratitude to B. Harasztos and A. Bacskovszky for linguistic revision of the manuscript.

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  1. Agricultural Research Institute of the Hungarian Academy of Sciences, P.O. Box 19, Martonvásár, 2462, Hungary

    Annamária Schneider, István Molnár & Márta Molnár-Láng

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  1. Annamária Schneider
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  2. István Molnár
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Correspondence to Márta Molnár-Láng.

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Schneider, A., Molnár, I. & Molnár-Láng, M. Selection of U and M genome-specific wheat SSR markers using wheat–Aegilops biuncialis and wheat–Ae. geniculata addition lines. Euphytica 175, 357–364 (2010). https://doi.org/10.1007/s10681-010-0180-5

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