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Utilization of SSR and cDNA markers for screening known QTLs for late blight (Phytophthora infestans) resistance in potato

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Abstract

All published QTLs and genes for Phytophthora resistance were projected onto the bins of a high-density reference map of potato. Further, a transcriptome map containing around 700 cDNA-AFLP (TDF) markers was anchored to this map. We have analysed cDNA markers which are co-located with these resistance QTLs by cloning, sequencing and by performing homology searches in public sequence databases. Several interesting homologies were detected with typical resistance and stress response genes. On the other hand, we have screened all known QTL locations on the 12 potato chromosomes via linked SSR markers for the presence of a selectable QTL for Phytophthora resistance in four genetic backgrounds. Progenies descended from different Solanum wild species as resistance sources. Leaf and tuber resistances were analysed. In all case studies, several selectable QTLs were detected which descended from either parent. Tuber and leaf QTLs varied from progeny to progeny and between leaves and tubers. In none of the progenies were resistance levels of leaf and tuber blight correlated.

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Acknowledgments

Part of this work was financed within the frame of EU projects QLK5-CT02-01849 (Apophys), FOOD-CT-2005-513959 (Bioexploit) and INIA project RTA2008-00045-C02-01.

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Authors and Affiliations

  1. NEIKER, Instituto Vasco de Investigación y Desarrollo Agrario, Aptdo. 46, Vitoria, Spain

    Enrique Ritter, Jose Ignacio Ruiz de Galarreta, Mónica Hernandez, Leire Barandalla, Raquel Lopez & Isbene Sanchez

  2. PROINPA, Fundación Promoción e Investigación de Productos Andinos, P.O. Box 4285, Cochabamba, Bolivia

    Giovanna Plata & Julio Gabriel

Authors
  1. Enrique Ritter
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  2. Jose Ignacio Ruiz de Galarreta
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  3. Mónica Hernandez
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  4. Giovanna Plata
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  5. Leire Barandalla
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  6. Raquel Lopez
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  7. Isbene Sanchez
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  8. Julio Gabriel
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Correspondence to Jose Ignacio Ruiz de Galarreta.

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Ritter, E., Ruiz de Galarreta, J.I., Hernandez, M. et al. Utilization of SSR and cDNA markers for screening known QTLs for late blight (Phytophthora infestans) resistance in potato. Euphytica 170, 77–86 (2009). https://doi.org/10.1007/s10681-009-9986-4

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  • DOI: https://doi.org/10.1007/s10681-009-9986-4

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