Development of triploid daylily (Hemerocallis) germplasm by embryo rescue

Abstract

Interploidy crosses between diploid daylily (Hemerocallis) cultivars (2n = 22) and tetraploid cultivars (2n = 44) were performed via in vivo hybridization aiming to produce triploid hybrid germplasm (3n = 33). Plant growth regulator-free MS based medium containing 5 levels of sucrose—1, 2, 3, 4 or 5% was used to optimize the embryo rescue medium. It was determined that the medium supplemented with 3% sucrose gave highest rate of immature hybrid embryo rescue. Thirty-seven hypothetically triploid genotypes were subjected to verification of ploidy status by root tip chromosome counting and flow cytometry. Thirty-one of them were confirmed triploids. These genotypes were in vitro propagated, acclimatized to ex vitro conditions, and planted for future field performance evaluation and environmental testing. The newly developed triploid genotypes could open new horizons for further polyploidy breeding.

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References

  1. AHS (2009) Online daylily cultivar database: http://www.daylilydatabase.org/. Am Hemerocallis Soc

  2. Arisumi T (1970) Experiments in breeding for triploid daylilies. Daylily J 24:33–37

    Google Scholar 

  3. Arisumi T (1973) Embryo development and seed set in crosses for triploid day Lilies. Bot Gaz 134:135–139. doi:10.1086/336693

    Article  Google Scholar 

  4. Bennett MD (2004) Perspectives on polyploidy in plants-ancient and neo. Biol J Linn Soc Lond 82:411–423. doi:10.1111/j.1095-8312.2004.00328.x

    Article  Google Scholar 

  5. Brennan JR (1992) Chromosomes of Hemerocallis. Daylily J 47:73–77

    Google Scholar 

  6. Brummer EC, Cazcarro PM, Luth D (1999) Ploidy determination of alfalfa germplasm accessions using flow cytometry. Crop Sci 39:1202–1207

    Article  Google Scholar 

  7. Costich DE, Ortiz R, Meagher TR, Bruederle LP, Vorsa N (1993) Determination of ploidy level and nuclear-DNA content in blueberry by flow-cytometry. Theor Appl Genet 86:1001–1006. doi:10.1007/BF00211053

    Article  CAS  Google Scholar 

  8. Dansi A, Mignouna HD, Pillay M, Zok S (2001) Ploidy variation in the cultivated yams (Dioscorea cayenensis-Dioscorea rotundata complex) from Cameroon as determined by flow cytometry. Euphytica 119:301–307. doi:10.1023/A:1017510402681

    Article  Google Scholar 

  9. Dark SOS (1932) Meiosis in diploid and triploid Hemerocallis. New Phytol 31:310–320. doi:10.1111/j.1469-8137.1932.tb06788.x

    Article  Google Scholar 

  10. Horn W (2002) Breeding for ornamentals. Kluwer Academic Publisher, Netherlands

    Google Scholar 

  11. Levin DA (1983) Polyploidy and Novelty in flowering plants. Am Nat 122:1–25. doi:10.1086/284115

    Article  Google Scholar 

  12. Matsuoka M (1971) Spontaneous occurrence of triploid Hemerocallis in Japan. Jpn J Breed 21:275–284

    Google Scholar 

  13. Morejohn LC, Bureau TE, Molè-Bajer J, Bajer AS, Fosket DE (1987) Oryzalin, a dinitroaniline herbicide, binds to plant tubulin and inhibits microtubule polymerization in vitro. Planta 172:252–264. doi:10.1007/BF00394595

    Article  CAS  Google Scholar 

  14. Munson RW (1989) Hemerocallis, the Daylily Journal. Timber Press, Portland, OR

    Google Scholar 

  15. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x

    Article  CAS  Google Scholar 

  16. Notzuka K, Tsuru T, Shiraishi M (2000) Included ployploidy via in vitro chromosome doubling. J Jpn Soc Hortic Sci 69:543–551

    Article  Google Scholar 

  17. Plodeck J (2002) The origin of the daylily cultivar traits. Hemerocallis Lett. 8:22–28

    Google Scholar 

  18. Raghavan V (2003) One hundred years of zygotic embryo culture investigations. In Vitro Cell Dev Biol Plant 39:437–442. doi:10.1079/IVP2003436

    Article  Google Scholar 

  19. Saito H, Mizunashia K, Tanakaa S, Adachia Y, Nakano M (2003) Ploidy estimation in Hemerocallis species and cultivars by flow cytometry. Sci Hortic (Amsterdam) 97:185–192. doi:10.1016/S0304-4238(02)00150-4

    Article  Google Scholar 

  20. Sakhanokho H, Cheatham C, Pounder JC (2004) Evaluation of techniques to induce polyploid daylilies. South Nurs Assoc Proc 49:591–594

    Google Scholar 

  21. Stout AB (1934) Daylilies: the wild species and garden clones, both old and new, of the genus Hemerocallis. Macmillan Press, New York

    Google Scholar 

  22. Tomkins JP, Wood TC, Barnes LS, Westman A, Wing RA (2001) Evaluation of genetic variation in the daylily (Hemerocallis spp.) using AFLP markers. Theor Appl Genet 102:489–496. doi:10.1007/s001220051672

    Article  Google Scholar 

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Acknowledgements

This project was funded by grants from Virginia Nursery and Landscape Association and the United States Department of Agriculture, as well as through operating funds provided by the Commonwealth of Virginia.

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Correspondence to Rumen Conev.

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Li, Z., Pinkham, L., Campbell, N.F. et al. Development of triploid daylily (Hemerocallis) germplasm by embryo rescue. Euphytica 169, 313–318 (2009). https://doi.org/10.1007/s10681-009-9958-8

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Keywords

  • Polyploidy
  • Cytology
  • Flow cytometry
  • Micropropagation
  • Tissue culture