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Genetic variability among sugarcane genotypes based on polymorphisms in sucrose metabolism and drought tolerance genes

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Abstract

Target region amplification polymorphism (TRAP) markers were used to estimate the genetic similarity (GS) among 53 sugarcane varieties and five species of the Saccharum complex. Seven fixed primers designed from candidate genes involved in sucrose metabolism and three from those involved in drought response metabolism were used in combination with three arbitrary primers. The clustering of the genotypes for sucrose metabolism and drought response were similar, but the GS based on Jaccard’s coefficient changed. The GS based on polymorphism in sucrose genes estimated in a set of 46 Brazilian varieties, all of which belong to the three Brazilian breeding programs, ranged from 0.52 to 0.9, and that based on drought data ranged from 0.44 to 0.95. The results suggest that genetic variability in the evaluated genes was lower in the sucrose metabolism genes than in the drought response metabolism ones.

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Abbreviations

Aqua:

Aquaporin

Arb:

Arbitrary primer

DBF:

Dehydration binding factor

DirH:

Dirigent protein related to high sugar content

DirL:

Dirigent protein related to low sugar content

LEA:

Late embryogenesis abundant protein

PIC:

Polymorphism information content

SAI:

Soluble acid invertase

SPS:

Sucrose phosphate synthase

Susy:

Sucrose synthase

Sut4 and Sut:

Sugar transporters

TRAP:

Target region amplified polymorphism

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Acknowledgments

This project was funded by Fundação de Amparo à Pesquisa do Estado de São Paulo (2005/56711-7) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (fellowship to Klaus A. G. Accoroni). The authors would like to give very special thanks to Vicente Eugênio de Rosa Junior, PhD (in memoriam), who directly participated in the choice of genes used to develop the work herewith.

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Authors and Affiliations

  1. Centro de Cana, Instituto Agronômico de Campinas, Anel Viário Contorno Sul, Km 321, CP 206, Ribeirão Preto, SP, 14001-970, Brazil

    Silvana Creste, Klaus A. G. Accoroni, Luciana R. Pinto, Mauro A. Xavier & Marcos G. A. Landell

  2. Departamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Av. Pádua Dias, 11, CP 83, Piracicaba, SP, 13400-970, Brazil

    Roland Vencovsky

  3. Embrapa Recursos Genéticos e Biotecnologia (CENARGEN), SAIN, PqEB, CP 02372, Brasília, CEP 70770-900, DF, Brazil

    Marcos A. Gimenes

Authors
  1. Silvana Creste
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  2. Klaus A. G. Accoroni
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  3. Luciana R. Pinto
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  4. Roland Vencovsky
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Correspondence to Silvana Creste.

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Creste, S., Accoroni, K.A.G., Pinto, L.R. et al. Genetic variability among sugarcane genotypes based on polymorphisms in sucrose metabolism and drought tolerance genes. Euphytica 172, 435–446 (2010). https://doi.org/10.1007/s10681-009-0078-2

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  • DOI: https://doi.org/10.1007/s10681-009-0078-2

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