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Selection for protein content in soybean from single F2 seed by near infrared reflectance spectroscopy

Selection protein by NIR in soybean

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Abstract

Soybean seed averages about 40% protein and is a dominant source of protein in animals and human foods. Increasing protein in soybeans is a goal in soybean breeding programs. Initial selection for protein among progeny from breeding populations is often based on non-destructive Near Infrared Reflectance (NIR) spectroscopy analysis of a 5 g or more seed sample in the F3 or later generations. NIR spectroscopy analysis for protein on single F2 seed would allow selection at the earliest possible time and improve selection efficiency for protein content; however this practice has not been evaluated. The objective of this study was to test effectiveness of NIR spectroscopy analysis of single F2 seeds to select for increased protein content from two populations developed from high and average protein content parents. F2 seeds from a single F1 plant of each population showed a normal distribution with transgressive segregation for protein concentration. In both populations, F3 seeds produced from plants from single F2 seeds with either low or high protein content were analyzed for protein by NIR spectroscopy. Protein means and ranges of F3 seed selected from high protein F2 seeds were higher in protein than F3 seed from low protein F2 seeds which produced low means and ranges in protein content. This shows that analysis of single F2 seed for protein content using NIR spectroscopy was effective in selecting for increased protein in the F3 generation. Analysis of single F2 seeds from breeding populations will improve breeding efficiency for protein in soybean breeding programs.

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Authors and Affiliations

  1. Division of Plant Biosciences, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Jeong-Dong Lee

  2. Division of Plant Sciences, University of Missouri-Delta Center, Portegeville, MO, 63873, USA

    J. Grover Shannon

  3. Department of Herbal Medicine Resource, Kangwon National University-Samcheok, Samcheok, 245-711, Republic of Korea

    Myoung-Gun Choung

Authors
  1. Jeong-Dong Lee
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  2. J. Grover Shannon
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  3. Myoung-Gun Choung
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Correspondence to Myoung-Gun Choung.

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Lee, JD., Shannon, J.G. & Choung, MG. Selection for protein content in soybean from single F2 seed by near infrared reflectance spectroscopy. Euphytica 172, 117–123 (2010). https://doi.org/10.1007/s10681-009-0067-5

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