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Potential new genes for resistance to Mycosphaerella graminicola identified in Triticum aestivum × Lophopyrum elongatum disomic substitution lines

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Abstract

Lophopyrum species carry many desirable agronomic traits, including disease resistance, which can be transferred to wheat by interspecific hybridization. To identify potentially new genes for disease and insect resistance carried by individual Lophopyrum chromosomes, 19 of 21 possible wheat cultivar Chinese Spring × Lophopyrum elongatum disomic substitution lines were tested for resistance to barley yellow dwarf virus (BYDV), cereal yellow dwarf virus (CYDV), the Hessian fly Mayetiola destructor, and the fungal pathogens Blumeria graminis and Mycosphaerella graminicola (asexual stage: Septoria tritici). Low resistance to BYDV occurred in some of the disomic substitution lines, but viral titers were significantly higher than those of two Lophopyrum species tested. This suggested that genes on more than one Lophopyrum chromosome are required for complete resistance to this virus. A potentially new gene for resistance to CYDV was detected on wheatgrass chromosome 3E. All of the substitution lines were susceptible to Mayetiola destructor and one strain of B. graminis. Disomic substitution lines containing wheatgrass chromosomes 1E and 6E were significantly more resistant to M. graminicola compared to Chinese Spring. Although neither chromosome by itself conferred resistance as high as that in the wheatgrass parent, they do appear to contain potentially new genes for resistance against this pathogen that could be useful for future plant-improvement programs.

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Acknowledgements

The authors wish to thank Jill Breeden, George Buechley, Brett Roberts, and Bryan Wallace for their technical contributions with the disease screening or molecular marker experiments. This work was supported by Purdue University project 3602-22000-014-03S and USDA-ARS CRIS projects 3602-22000-012-00D, 3602-21220-013-00D, and 3602-21220-008-00D. Support for G.G. was provided by the Hungarian-American Fulbright Commission, Grant No. 1201 102.

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Authors and Affiliations

  1. Crop Production and Pest Control Research Unit, U. S. Department of Agriculture-Agricultural Research Service, Purdue University, 915 West State Street, West Lafayette, IN, 47907-2054, USA

    Joseph M. Anderson, Dennis L. Bucholtz, Christie E. Williams & Stephen B. Goodwin

  2. Department of Entomology, Purdue University, 901 West State St., West Lafayette, IN, 47907-2089, USA

    Nagesh Sardesai

  3. Department of Agronomy, Purdue University, 915 West State St., West Lafayette, IN, 47907-2054, USA

    Judith B. Santini

  4. Institute of Genetics and Biotechnology, HAS-SIU, Saint Stephanus University, Gödöllő, H-2103, Hungary

    Gábor Gyulai

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  1. Joseph M. Anderson
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  2. Dennis L. Bucholtz
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  3. Nagesh Sardesai
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  4. Judith B. Santini
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  5. Gábor Gyulai
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  6. Christie E. Williams
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  7. Stephen B. Goodwin
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Correspondence to Stephen B. Goodwin.

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Names are necessary to report factually on available data. However, the USDA neither guarantees nor warrants the standard of the product, and the use of the name implies no approval of the product to the exclusion of others that also may be suitable.

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Anderson, J.M., Bucholtz, D.L., Sardesai, N. et al. Potential new genes for resistance to Mycosphaerella graminicola identified in Triticum aestivum × Lophopyrum elongatum disomic substitution lines. Euphytica 172, 251–262 (2010). https://doi.org/10.1007/s10681-009-0061-y

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  • DOI: https://doi.org/10.1007/s10681-009-0061-y

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