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Genetic analysis reveals a dominant S locus and an S suppressor locus in natural self-compatible Brassica napus

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Abstract

A self-incompatible (SI) line, S-1300, and its maintainer 97-wen135, a self-compatible (SC) line, were used to study the inheritance of maintenance for self-incompatibility in B. napus. The ratio of SI plants to SC plants from S-1300 × 97-wen135 F2 and (S-1300 × 97-wen135) × 97-wen135 was 346:260 and 249:232, fitting the expected ratio of 9:7 and 1:1, respectively. Based on these observations, here we propose a genetic model in which two independent loci, S locus and S suppressor locus (sp), are predicted to control the inheritance of maintenance for self-incompatibility in B. napus. The genotypes of S-1300 and 97-wen135 are S 1300 S 1300 sp 1300 sp 1300 and S 135 S 135 sp 135 sp 135 , respectively. S 135 is dominant to S 1300 , but coexistence of sp 1300 and sp 135 fails to suppress S locus. Both S 1300 and S 135 can be suppressed by sp 135 , while sp 1300 can suppress S 135 but not S 1300 . The model contains two characteristics: that a dominant S locus exists in self-compatible B. napus, and that co-suppression will occur when sp loci are heterozygous. The model has been validated by the segregation of S phenotypes in the (S-1300 × 97-wen135) × S-1300, the progenies of SC S-1300 × 97-wen135 F2 plants and DH population developed from S-1300 × 97-wen135 F1. This is the first study to report co-suppression of S suppressor loci in B. napus. The genetic model will be very useful for developing molecular markers linked to maintenance for self-incompatibility and for dissecting the mechanism of SI/SC in B. napus.

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Acknowledgments

The authors are grateful to Wallace Cowling of the University of Western Australia, and several anonymous reviewers, for helpful suggestions in revising the manuscript. This research was financed by funds from the National Key Basic Research Special Foundation of China (2007CB1090) and supported by the Industry Technology System of Rapeseed in China (NYCYTX-00501).

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  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Chaozhi Ma, Chunyan Li, Yongqiang Tan, Wei Tang, Jianfeng Zhang, Changbin Gao & Tingdong Fu

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  1. Chaozhi Ma
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  2. Chunyan Li
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  3. Yongqiang Tan
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  4. Wei Tang
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  7. Tingdong Fu
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Correspondence to Chaozhi Ma.

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Ma, C., Li, C., Tan, Y. et al. Genetic analysis reveals a dominant S locus and an S suppressor locus in natural self-compatible Brassica napus . Euphytica 166, 123–129 (2009). https://doi.org/10.1007/s10681-008-9846-7

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