Abstract
Fusarium head blight (FHB), or scab, is a devastating wheat disease worldwide, reducing both grain yield and quality. The percentage of Fusarium-damaged kernels (FDK) directly reflects the damage level caused by scab on wheat grains and its variation represents the so-called type IV scab resistance in germplasm. To identify genes governing type IV resistance and investigate its relationship with other scab resistance types, we mapped QTLs associated with percent FDK using data from three different field evaluations of the recombination inbred line (RIL) population derived from the susceptible cultivar Nanda 2419 × the scab-resistant cultivar Wangshuibai. Five QTLs related to percent FDK were identified in at least two different trials, for which Wangshuibai contributed four of the resistance alleles. Most of the FDK-related QTLs, including the three with larger effects, QFdk.nau-2B, QFdk.nau-3B and QFdk.nau-4B, mapped to intervals associated with either type IV resistance or type II resistance. Moreover, most of the major type I and type II resistance QTLs detected previously were associated with type IV resistance, suggesting that resistance to initial infection and disease spread play major roles in conditioning less FDK. Therefore, breeders have options to choose inoculation methods based on their expertise and resources without risking significant loss of information when using percent FDK as the disease index. The most useful scab resistance QTLs for breeding would be those with stable influences on FDK and/or deoxynivalenol (DON) accumulation besides the initial infection and disease spread.
Similar content being viewed by others
References
Anderson JA, Stack RW, Liu S, Waldron BL, Field AD, Coyne C, Moreno-Sevilla B, Mitchell-Fetch J, Song QJ, Cregan PB, Frohberg RC (2001) DNA markers for Fusarium head blight resistance QTLs in two wheat populations. Theor Appl Genet 102:1164–1168
Bai GH, Kolb FL, Shaner G, Domier LL (1999) Amplified fragment length polymorphism markers linked to a major quantitative trait locus controlling scab resistance in wheat. Phytopathology 89:343–348
Bai GH, Shaner GE (2004) Management and resistance in wheat and barley to Fusarium head blight. Annu Rev Phytopathol 42:135–161
Bai GH, Shaner G, Ohm H (2000) Inheritance of resistance to Fusarium graminearum in wheat. Theor Appl Genet 100:1–8
Bai GH, Plattner R, Desjardins A, Kolb F (2001) Resistance to Fusarium head blight and deoxynivalenol in wheat. Plant Breed 120:1–6
Buerstmayr H, Steiner B, Lemmens M, Ruckenbauer P (2000) Resistance to Fusarium head blight in winter wheat: heritability and trait associations. Crop Sci 40:1012–1018
Buerstmayr H, Lemmens M, Hartl L, Doldi L, Steiner B, Stierschneider M, Ruckenbauer P (2002) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (Type II resistance). Theor Appl Genet 104:84–91
Chen J, Griffey CA, Maroof MAS, Stromberg EL, Biyashev RM, Zhao W, Chappell MR, Pridgen TH, Dong Y, Zeng Z (2006) Validation of two major quantitative trait loci for Fusarium head blight resistance in Chinese wheat line W14. Plant Breed 125:99–101
Gervais L, Dedryver F, Morlais JY, Bodusseau V, Negre S, Bilous M, Groos C, Trottet M (2003) Mapping of quantitative trait loci for field resistance to Fusarium head blight in an European winter wheat. Theor Appl Genet 106:961–970
Gilsinger J, Kong L, Shen X, Ohm H (2005) DNA markers associated with low Fusarium head blight incidence and narrow flower opening in wheat. Theor Appl Genet 110:1218–1225
Han FP, Fedak G, Ouellet T, Dan H, Somers DJ (2005) Mapping of genes expressed in Fusarium graminearum-infected heads of wheat cultivar ‘Frontana’. Genome 48:88–96
Lander ES, Botstein D (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121:185–199
Lin F, Kong ZX, Zhu HL, Xue SL, Wu JZ, Tian DG, Wei JB, Zhang CQ, Ma ZQ (2004) Mapping QTL associated with resistance to Fusarium head blight in the Nanda 2419 × Wangshuibai population. I. Type II resistance. Theor Appl Genet 109:1504–1511
Lin F, Xue SL, Zhang ZZ, Zhang CQ, Kong ZX, Yao GQ, Tian DG, Zhu HL, Li CJ, Cao Y, Wei JB, Luo QY, Ma ZQ (2006) Mapping QTL associated with resistance to Fusarium head blight in the Nanda 2419 × Wangshuibai population. II: Type I resistance. Theor Appl Genet 112:528–535
Ma HX, Zhang KM, Gao L, Bai GH, Chen HG, Cai ZX, Lu WZ (2006) Quantitative trait loci for resistance to Fusarium head blight and deoxynivalenol accumulation in Wangshuibai wheat under field conditions. Plant Pathol 55:739–745
Ma ZQ, Zhao DM, Zhang CQ, Zhang ZZ, Xue SL, Lin F, Kong ZX, Tian DG, Luo QY (2007) Genetic analysis of five spike-related traits in wheat using RIL and immortalized F2 populations. Mol Genet Genomics 277:31–42
McMullen M, Jones R, Gallenberg D (1997) Scab of wheat and barley: a re-emerging disease of devastating impact. Plant Dis 81:1340–1348
Mesterhazy A (1995) Types and components of resistance to Fusarium head blight of wheat. Plant Breed 114:377–386
Miller JD, Young JC, Sampson DR (1985) Deoxynivalenol and Fusarium head blight resistance in spring cereals. J Phytopathol 113:359–367
Paillard S, Schnurbusch T, Tiwari R, Messmer M, Winzeler M, Keller B, Schachermayr G (2004) QTL analysis of resistance to Fusarium head blight in Swiss winter wheat (Triticum aestivum L.). Theor Appl Genet 109:323–332
Paul PA, Lipps PE, Madden LV (2005) Relationship between visual estimates of Fusarium head blight intensity and deoxynivalenol accumulation in harvested wheat grain: a meta-analysis. Phytopathogy 95:1225–1236
Schmolke M, Zimmermann G, Buerstmayr H, Schweizer G, Miedaner T, Korzun V, Ebmeyer E, Hartl L (2005) Molecular mapping of Fusarium head blight resistance in the winter wheat population Dream/Lynx. Theor Appl Genet 111:747–756
Schroeder HW, Christensen JJ (1963) Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53:831–838
Schuhmacher R, Krska R, Weingaertner J, Grasserbauer M (1997) Interlaboratory comparison study for the determination of the Fusarium mycotoxins deoxynivalenol in wheat and zearalenone in maize using different methods. Fresenius J Anal Chem 359:510–515
Somers DJ, Fedak G, Savard M (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat. Genome 46:555–564
Steiner B, Lemmens M, Griesser M, Scholz U, Schondelmaier J, Buerstmayr H (2004) Molecular mapping of resistance to Fusarium head blight in the spring wheat cultivar Frontana. Theor Appl Genet 109:215–224
Yang ZP, Gilbert J, Somers DJ, Fedak G, Procunier JD, McKenzie IH (2003) Marker assisted selection of Fusarium head blight resistance genes in two doubled haploid populations of wheat. Mol Breed 12:309–317
Yang ZP, Gilbert J, Fedak G, Somers DJ (2005) Genetic characterization of QTL associated with resistance to Fusarium head blight in a doubled haploid spring wheat population. Genome 48:187–196
Zhou WC, Kolb FL, Bai G, Shaner G, Domier LL (2002) Genetic analysis of scab resistance QTL in wheat with microsatellite and AFLP markers. Genome 45:719–727
Acknowledgements
This project was partially supported by NFSC program (30671295, 30270807, 30430440), NSFC-outstanding youth fund, ‘973’ program, and ‘863’ program (2002AA224161, 2003AA207100). We thank all the lab staff and graduate students who participated in the work.
Author information
Authors and Affiliations
Corresponding author
Additional information
Chunjun Li and Huilan Zhu contributed equally to this work.
Rights and permissions
About this article
Cite this article
Li, C., Zhu, H., Zhang, C. et al. Mapping QTLs associated with Fusarium-damaged kernels in the Nanda 2419 × Wangshuibai population. Euphytica 163, 185–191 (2008). https://doi.org/10.1007/s10681-007-9626-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10681-007-9626-9