Skip to main content
Log in

Stability of soil plant analytical development (SPAD) chlorophyll meter reading (SCMR) and specific leaf area (SLA) and their association across varying soil moisture stress conditions in groundnut (Arachis hypogaea L.)

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

The complex nature of physiological traits associated with drought tolerance and the difficulties associated with their measurements in segregating populations and large number of genotypes inhibited their use in the past in developing water-use efficient genotypes in breeding programmes. With new knowledge of easily measurable surrogates of transpiration efficiency (TE), a trait associated with drought tolerance—specific leaf area (SLA) and soil plant analytical development (SPAD) chlorophyll meter reading (SCMR), it is now possible to integrate TE through the surrogates in breeding and selection schemes in groundnut (Arachis hypogaea L.). As a noninvasive surrogate of TE, SCMR is easy to operate, reliable, fairly stable and low cost. However, in a large-scale breeding program, it is difficult to complete SCMR observations within a specified time. The present study addressed the issue as to what extent the SCMR measurements can be spread over time by evaluating 18 diverse groundnut genotypes for two physiological traits, SCMR and SLA in two postrainy (Nov–Apr) seasons (2002/2003 and 2003/2004) in India. Observations were recorded at different times during and after the release of moisture deficit stress. There was general agreement in genotype and trait performance in both the seasons. Interaction between SCMR and time of observation was significant in only one season (2002/2003) but its variance relative to genotypes and time of observation was very small. ICGV 99029 and ICR 48, which recorded higher SCMR and lower SLA values in both the seasons, will make good parents for water-use efficiency trait in breeding programmes. Other good parents include ICGS 76, TCGS 647 and TCGP 6. SCMR recorded at three different times under differing soil moisture deficit in each season showed highly significant correlation with each other. Similarly, SLA at different times also correlated significantly with each other. SCMR and SLA were significantly negatively correlated with each other and the relationship was insensitive to time of observation. The results of the present study indicated that SCMR/SLA observations can be recorded at any time after 60 days of crop growth, preferably under moisture deficit conditions. This gives groundnut breeders a large flexibility to record these observations in a large number of segregating populations and breeding lines in the field. Thus, making it easy to incorporate these physiological traits associated with drought tolerance in breeding and selection scheme in groundnut.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bindu Madhava H, Sheshshayee MS, Shankar AG, Prasad TG, Udayakumar M (2003) Use of SPAD chlorophyll meter to assess transpiration efficiency of peanut. In: Cruickshank AW, Rachaputi NC, Wright GC, Nigam SN (eds) Breeding of drought resistant peanuts. ACIAR Proceedings No. 112. Australian Centre for International Agricultural Research, Canberra, pp 3–9

  • Cruickshank AW, Wright GC, Rachaputi NC, Foster S (2003). Multi-environment analysis for Queensland sites. In: Cruickshank AW, Rachaputi NC, Wright GC, Nigam SN (eds) Breeding of drought resistant peanuts. ACIAR Proceedings No. 112. Australian Centre for International Agricultural Research, Canberra, pp 72–76

  • Hebbar KB, Sashidhar VR, Udayakumar M, Devendra R, Nageswara Rao RC (1994) A comparative assessment of water use efficiency in groundnut (Arachis hypogaea) grown in containers and in the field under water-limited conditions. J Agric Sci (Cambridge) 122:429–434

    Google Scholar 

  • Hubick KT, Farquhar GD, Shorter R (1986) Correlation between water-use efficiency and carbon isotope discrimination in diverse peanut germplasm. Aust J Plant Physiol 13:803–816

    CAS  Google Scholar 

  • Lal C, Hariprasanna K, Rathnakumar AL, Samdur MY (2006) High yielding, water use efficient Spanish groundnut (Arachis hypogaea) genotypes for rainfed production system. Indian J Agric Sci 76:148–150

    Google Scholar 

  • Nageswara Rao RC, Talwar HS, Wright GC (2001) Rapid assessment of specific leaf area and leaf N in peanut (Arachis hypogaea L.) using chlorophyll meter. J Agron Crop Sci 189:175–182

    Article  Google Scholar 

  • Nageswara Rao RC, Williams JH, Wadia KDR, Hubick KT, Farquhar GD (1993) Crop growth, water-use efficiency and carbon isotope discrimination in groundnut (Arachis hypogaea L.) genotypes under end-of-season drought conditions. Ann Appl Biol 122:357–367

    Article  Google Scholar 

  • Nageswara Rao RC, Wright GC (1994). Stability of the relationship between specific leaf area and carbon isotope discrimination across environments in peanut. Crop Sci 34:98–103

    Article  Google Scholar 

  • Nigam SN, Chandra S, Rupa Sridevi K, Manohar B, Reddy AGS, Nageswara Rao RC, Wright GC, Reddy PV, Deshmukh MP, Mathur RK, Basu MS, Vasundhara S, Vindhiya Varman P, Nagda AK (2005) Efficiency of physiological trait-based and empirical selection approaches for drought tolerance in groundnut. Ann Appl Biol 146:433–439

    Article  Google Scholar 

  • Passioura JB (1977) Grain yield, harvest index and water use of wheat. J Aust Inst Agric Sci 43:117–120

    Google Scholar 

  • Richardson AD, Duigan SP, Berlyn GP (2002) An evaluation of noninvasive methods to estimate foliar chlorophyll content. New Phytol 153:185–194

    Article  CAS  Google Scholar 

  • Reddy PV, Sudhakar P, Sujatha D, Babitha M, Latha P (2004) Relationship of SPAD chlorophyll meter reading with specific leaf area, leaf total chlorophyll and pod yield in groundnut (Arachis hypogaea L.). In: Reddy PV, Rao JSP, Reddy KB, Sudhakar P, Rama Rao G, Babitha M, Latha P, Jyotsna MK (eds) Proceedings of the national seminar on physiological interventions for improved crop productivity and quality: opportunities and constraints. Indian Society for Plant Physiology, Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, India, pp 24–27

  • Serraj R, Krishnamurthy L, Devi MJ, Reddy MJV, Nigam SN (2004) Variation in transpiration efficiency and related traits in a groundnut mapping population. Int Arachis Newsl 24:42–45

    Google Scholar 

  • Sheshshayee MS, Bindumadhava H, Rachaputi NR, Prasad TG, Udayakumar M, Wright GC, Nigam SN (2006) Leaf chlorophyll concentration relates to transpiration efficiency in peanut. Ann Appl Biol 148:7–15

    Article  CAS  Google Scholar 

  • Upadhyaya HD (2005) Variability for drought resistance related traits in the mini core collection of peanut. Crop Sci 45:1432–1440

    Article  Google Scholar 

  • Winer BJ (1962) Statistical principles in experimental design, 2nd edn. McGraw-Hill, New York

    Google Scholar 

  • Wright GC, Hubick KT, Farquhar GD (1988) Discrimination in carbon isotopes of leaves correlates with water-use efficiency of field grown peanut cultivars. Aust J Plant Physiol 15:815–825

    Article  Google Scholar 

  • Wright GC, Hubick KT, Farquhar GD, Nageswara Rao RC (1993) Genetic and environmental variation in transpiration efficiency and its correlation with carbon isotope discrimination and specific leaf area in peanut. In: Ehleringer JR, Hall AE, Farquhar GD (eds) Stable isotopes and plant carbon-water relations. Academic Press Inc., San Diego, pp 247–267

    Google Scholar 

  • Wright GC, Nageswara Rao RC, Farquhar GD (1994) Water-use efficiency and carbon isotope discrimination under water deficit conditions. Crop Sci 34:92–97

    Article  Google Scholar 

Download references

Acknowledgements

The financial assistance provided by the Australian Centre for International Agricultural Research under the project, ‘Selection for peanut varieties with low aflatoxin risk (PHT/2000/080)’ is gratefully acknowledged. The technical assistance in recording observations and day to day management of trials by M Bhukta and AGS Reddy is appreciated.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Shyam Narayan Nigam.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nigam, S.N., Aruna, R. Stability of soil plant analytical development (SPAD) chlorophyll meter reading (SCMR) and specific leaf area (SLA) and their association across varying soil moisture stress conditions in groundnut (Arachis hypogaea L.). Euphytica 160, 111–117 (2008). https://doi.org/10.1007/s10681-007-9581-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-007-9581-5

Keywords

Navigation