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Identification and genetic mapping of a powdery mildew resistance gene in wild emmer (Triticum dicoccoides) accession IW72 from Israel

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Abstract

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease of wheat (Triticum aestivum) in China and worldwide, causing severe yield losses annually. Wild emmer (T. dicoccoides) accession IW72 collected from Israel is resistant to powdery mildew at the seedling and adult stages. Genetic analysis indicated that the resistance was controlled by a single dominant gene, temporarily designated MlIW72. The F2 population and F3 families derived from a hybrid between IW72 and susceptible durum wheat line Mo75 were used for molecular mapping of the resistance gene. MlIW72 was linked with SSR loci Xgwm344, Xcfa2040, Xcfa2240, Xcfa2257 and Xwmc525 on the long arm of chromosome 7A. In addition, two STS markers, MAG2185 (derived from RFLP marker PSR680) and MAG1759 (developed from EST CD452874), were mapped close to MlIW72. All these markers were physically located in the terminal bin 0.86–1.00 of 7AL. The chromosome location and genetic mapping results suggested that the powdery mildew resistance gene identified in wild emmer accession IW72 might be a new allele at the Pm1 locus or a new locus closely linked to Pm1.

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Acknowledgements

The authors thank Dr. Robert McIntosh for his comprehensive review of this manuscript. This work was financially supported by the National Natural Science Foundation of China (30571151), National Fund for Distinguished Young Scholars (30425039), State High-Tech Programs (2006AA100102, 2006AA10Z1C4, 2006AA10Z1E9) Fok Ying Tung Education Foundation (94021), and Beijing Natural Science Foundation (6061003).

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Authors and Affiliations

  1. College of Agronomy and Biotechnology, State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture, Beijing Key Laboratory of Crop Genetic Improvement, Key Laboratory of Crop Heterosis Research, Untilization, Ministry of Education, China Agricultural University, Beijing, 100094, P.R. China

    Xiaoling Ji, Chaojie Xie, Zhongfu Ni, Tsomin Yang, Zhiyong Liu & Qixin Sun

  2. Institute of Evolution, University of Haifa, Mt. Carmel, Haifa, 31905, Israel

    Eviatar Nevo & Tzion Fahima

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  1. Xiaoling Ji
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  2. Chaojie Xie
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  3. Zhongfu Ni
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  5. Eviatar Nevo
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  6. Tzion Fahima
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  7. Zhiyong Liu
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Correspondence to Chaojie Xie.

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Ji, X., Xie, C., Ni, Z. et al. Identification and genetic mapping of a powdery mildew resistance gene in wild emmer (Triticum dicoccoides) accession IW72 from Israel. Euphytica 159, 385–390 (2008). https://doi.org/10.1007/s10681-007-9540-1

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  • DOI: https://doi.org/10.1007/s10681-007-9540-1

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