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Microsatellite mapping of powdery mildew resistance allele Pm5d from common wheat line IGV1-455

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Abstract

The powdery mildew resistance allele Pm5d in the backcross-derived wheat lines IGV1-455 (CI10904/7*Prins) and IGV1-556 (CI10904/7*Starke) shows a wide spectrum of resistance and virulent pathotypes have not yet been detected in Germany. Although this allele may be distinguished from the other documented Pm5 alleles by employing a differential set of Blumeria graminis tritici isolates, the use of linked molecular markers could enhance selection, especially for gene pyramiding. Pm5d was genetically mapped relative to six microsatellite markers in the distal part of chromosome 7BL using 82 F3 families of the cross Chinese Spring × IGV1-455. Microsatellite-based deletion line mapping placed Pm5d in the terminal 14% of chromosome 7BL. The closely linked microsatellite markers Xgwm577 and Xwmc581 showed useful variation for distinguishing the different Pm5 alleles except the ones originating from Chinese wheat germplasm. Their use, however, would be limited to particular crosses because they are not functional markers. The occurrence of resistance genes closely linked to the Pm5 locus is discussed.

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References

  • Anonymous (2006) Beschreibende Sortenliste, Deutscher Landwirtschaftsverlag GmbH, Hannover, Germany

  • Endo TR, Gill BS (1996) The deletion stocks of common wheat. J Hered 87:295–307

    CAS  Google Scholar 

  • Hohmann U, Endo TR, Gill KS, Gill BS (1994) Comparison of genetic and physical maps of group-7 chromosomes from Triticum aestivum L. Mol Gen Genet 245:644–653

    Article  PubMed  CAS  Google Scholar 

  • Hsam SLK, Huang XQ, Zeller FJ (2001) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em. Thell.). 6. Alleles at the Pm5 locus. Theor Appl Genet 102:127–133

    Article  CAS  Google Scholar 

  • Huang XQ, Röder MS (2004) Molecular mapping of powdery mildew resistance genes in wheat: a review. Euphytica 137:203–223

    Article  CAS  Google Scholar 

  • Huang XQ, Hsam SLK, Zeller FJ (1997) Identification of powdery mildew resistance genes in common wheat (Triticum aestivum L.). IX. Cultivars, landraces and breeding lines grown in China. Plant Breed 116:233–238

    Article  Google Scholar 

  • Huang XQ, Hsam SLK, Zeller FJ (2000a) Chromosomal location of powdery mildew resistance genes in Chinese wheat (Triticum aestivum L. em. Thell.) landraces Xiaobaidong and Fuzhuang 30. J Genet Breed 54:311–317

    CAS  Google Scholar 

  • Huang XQ, Zeller FJ, Hsam SLK et al (2000b) Chromosomal location of AFLP markers in common wheat utilizing nulli-tetrasomic stocks. Genome 43:298–305

    Article  PubMed  CAS  Google Scholar 

  • Huang XQ, Hsam SLK, Zeller FJ et al (2000c) Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding. Theor Appl Genet 101:407–414

    Article  CAS  Google Scholar 

  • Huang XQ, Hsam SLK, Zeller FJ (2002) Chromosomal location of genes for resistance to powdery mildew in Chinese wheat lines Jieyan 94-1-1 and Siyan 94-1-2. Hereditas 136:212–218

    Article  PubMed  Google Scholar 

  • Huang XQ, Wang LX, Xu MX, Röder MS (2003) Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.). Theor Appl Genet 106:858–865

    PubMed  CAS  Google Scholar 

  • Ishii T, Yonezawa K (2007) Optimization of the marker-based procedures for pyramiding genes from multiple donor lines: I schedule of crossing between the donor lines. Crop Sci 47:537–546

    Article  Google Scholar 

  • Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugen 12:172–175

    Google Scholar 

  • Law CN, Wolfe MS (1966) Location of genetic factors for mildew resistance and ear emergence time on chromosome 7B of wheat. Can J Genet Cytol 8:462–470

    Google Scholar 

  • Lebsock KL, Briggle LW (1974) Gene Pm5 for resistance to Erysiphe graminis f. sp. tritici in Hope wheat. Crop Sci 14:561–563

    Article  Google Scholar 

  • Miranda LM, Murphy JP, Marshall D, Leath S (2006) Pm34: a new powdery mildew resistance gene transferred from Aegilops tauschii Coss. to common wheat (Triticum aestivum L). Theor Appl Genet 113:1497–1504

    Article  PubMed  CAS  Google Scholar 

  • Mohler V, Zeller FJ, Wenzel G, Hsam SLK (2005) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.). 9. Gene MlZec1 from the Triticum dicoccoides-derived wheat line Zecoi-1. Euphytica 142:161–167

    Article  CAS  Google Scholar 

  • Shi AN, Leath S, Murphy JP (1998) A major gene for powdery mildew resistance transferred to common wheat from wild einkorn wheat. Phytopathology 88:144–147

    Article  CAS  PubMed  Google Scholar 

  • Singrün C, Hsam SLK, Zeller FJ et al (2004) Localization of a novel recessive powdery mildew resistance gene from common wheat line RD30 in the terminal region of chromosome 7AL. Theor Appl Genet 109:210–214

    Article  PubMed  CAS  Google Scholar 

  • Srnić G, Murphy JP, Lyerly JH et al (2005) Inheritance and chromosomal assignment of powdery mildew resistance genes in two winter wheat germplasm lines. Crop Sci 45:1578–1586

    Article  CAS  Google Scholar 

  • Sun XL, Liu D, Zhang HQ et al (2006) Identification and mapping of two new genes conferring resistance to powdery mildew from Aegilops tauschii (Coss.) Schmal. J Integr Plant Biol 48:1204–1209

    Article  CAS  Google Scholar 

  • Van Ooijen JW, Voorrips RE (2001) Joinmap 30, software for the calculation of genetic linkage maps. Plant Research International, Wageningen, The Netherlands

    Google Scholar 

  • Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78

    Article  PubMed  CAS  Google Scholar 

  • Werner JE, Endo TR, Gill BS (1992) Toward a cytogenetically based physical map of the wheat genome. Proc Natl Acad Sci USA 89:11307–11311

    Article  PubMed  CAS  Google Scholar 

  • Yahiaoui N, Brunner S, Keller B (2006) Rapid generation of new powdery mildew resistance genes after wheat domestication. Plant J 47:85–98

    Article  PubMed  CAS  Google Scholar 

  • Yao G, Zhang JL, Yang LL et al (2007) Genetic mapping of two powdery mildew resistance genes in einkorn (Triticum monococcum L.) accessions. Theor Appl Genet 114:351–358

    Article  PubMed  CAS  Google Scholar 

  • Zeller FJ, Huang XQ, Paderina EV et al (1998) Identification of powdery mildew resistance genes in common wheat (Triticum aestivum L. em. Thell.). XII. Cultivars and landraces grown in Mediterranean countries. Plant Genet Resour Newsl 116:5–8

    Google Scholar 

  • Zhu ZD, Zhou RH, Kong XY et al (2005) Microsatellite markers linked to 2 powdery mildew resistance genes introgressed from Triticum carthlicum accession PS5 into common wheat. Genome 48:585–590

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

The authors would like to thank Prof. Bob McIntosh for substantially improving the manuscript.

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Correspondence to Volker Mohler.

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Ghazaleh Nematollahi and Volker Mohler equally contributed to this work.

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Nematollahi, G., Mohler, V., Wenzel, G. et al. Microsatellite mapping of powdery mildew resistance allele Pm5d from common wheat line IGV1-455. Euphytica 159, 307–313 (2008). https://doi.org/10.1007/s10681-007-9494-3

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  • DOI: https://doi.org/10.1007/s10681-007-9494-3

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