Skip to main content
SpringerLink
Log in

SCAR and CAPS markers flanking the Brassica oleracea L. Pp523 downy mildew resistance locus demarcate a genomic region syntenic to the top arm end of Arabidopsis thaliana L. chromosome 1

  • Published:
Euphytica Aims and scope Submit manuscript

Abstract

We recently mapped the Pp523 locus that includes a single, dominant gene conferring resistance to downy mildew expressed in adult plants to a 75.1 cm long linkage group on a genetic linkage map of Brassica oleracea L. More recently, we identified a new AFLP marker 2.8 cm downstream from the resistance gene. The five DNA markers within an 8.5 cm region encompassing the Pp523 gene were cloned and sequenced. Three of these markers were transformed into SCARs (sequence characterised amplified regions), however, two among them were monomorphic and were analysed as CAPS (cleaved amplified polymorphic sequence) markers among the mapping population. Searched against genomic databases, the five B. oleracea DNA-marker sequences matched Arabidopsis thaliana L. gene sequences that delimit a conserved syntenic region in the top arm end of chromosome 1 of this last species. Considering the close genetic relatedness between both species, the information on this specific genomic region in A. thaliana is particularly useful for the construction of a fine-scale map of the corresponding genomic region in B. oleracea. The identified SCAR and CAPS markers can be used for marker assisted selection (MAS) in breeding programs aimed at the introgression of the Pp523 resistance locus, allowing the reliable indirect identification of plants harbouring the resistance gene with a margin of error of approximately six in ten-thousand selected plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Agnola B, Silue D, Boury S (2000) Identification of RAPD markers of downy mildew (Peronospora parasitica) resistance gene in broccoli (Brassica oleracea var. italica). In: GJ King (ed) Proceedings of the 3rd ISHS international symposium on Brassicas, Horticulture Research International, Wellesbourne, 5–9 September 2000, p. 64

  • Ayele M, Haas BJ, Kumar N, Wu H, Xiao Y, Van Aken S, Utterback TR, Wortman JR, White OR, Town CD (2005) Whole genome shotgun sequencing of Brassica oleracea and its application to gene discovery and annotation in Arabidopsis. Genome Res 15:487–495

    Article  PubMed  Google Scholar 

  • Babula D, Kaczmarek M, Barakat A, Delseny M, Quiros CF, Sadowski J (2003) Chromosomal mapping of Brassica oleracea based on ESTs from Arabidopsis thaliana: complexity of the comparative map. Mol Genet Genomics 268:656–665

    PubMed  CAS  Google Scholar 

  • Babula D, Misztal LH, Jakubowicz M, Kaczmarek M, Nowak W, Sadowski J (2006) Genes involved in biosynthesis and signalisation of ethylene in Brassica oleracea and Arabidopsis thaliana: identification and genome comparative mapping of specific gene homologues. Theor Appl Genet 112:410–420

    Article  PubMed  CAS  Google Scholar 

  • Bittner-Eddy PD, Crute IR, Holub EB, Beynon JL (2000) RPP13 is a simple locus in Arabidopsis thaliana for alleles that specify downy mildew resistance to different avirulence determinants in Peronospora parasitica. Plant J 21:177–188

    Article  PubMed  CAS  Google Scholar 

  • Coelho PS, Monteiro A (2003a) Inheritance of downy mildew resistance in mature broccoli plants. Euphytica 131:65–69

    Article  Google Scholar 

  • Coelho PS, Monteiro A (2003b) Expression of resistance to downy mildew at cotyledon and adult plant stages in Brassica oleracea L. Euphytica 133:279–284

    Article  Google Scholar 

  • Constantinescu O, Fatehi J (2002) Peronospora-like fungi (Chromista peronosporales) parasitic on Brassicaceae and related hosts. Nova Hedwigia 74:291–334

    Article  Google Scholar 

  • Farinhó M, Coelho P, Carlier J, Svetleva D, Monteiro A, Leitão J (2004) Mapping of a locus for adult plant resistance to downy mildew in broccoli (Brassica oleracea convar. italica). Theor Appl Genet 109:1392–1398

    Article  PubMed  Google Scholar 

  • Farinhó MJ, Coelho PS, Monteiro AA, Leitão JM (2000) RAPD and AFLP markers linked to Peronospora parasitica resistance genes in broccoli. In: King GJ (ed) Proceedings of the 3rd ISHS International Symposium on Brassicas, Horticulture Research International, Wellesbourne, 5–9 September 2000, p75

  • Farnham MW, Wang M, Thomas CE (2002) A single dominant gene for downy mildew resistance in broccoli. Euphytica 128:405–407

    Article  CAS  Google Scholar 

  • Gao M, Li G, Yang B, Farnham M, Quiros C (2003) Search for candidate genes for downy mildew resistance in broccoli. In: abstracts of the plant and animal genomes XI conference, Town and Country Convention Center, San Diego, 11–15 January 2003, p476

  • Gao M, Li G, McCombie W, Quiros C (2005) Comparative analysis of a transposon-rich Brassica oleracea BAC clone with its corresponding sequence in A. thaliana. Theor Appl Genet 111:949–955

    Article  PubMed  CAS  Google Scholar 

  • Giovannelli JL, Farnham MW, Wang M (2002) Development of sequence characterized amplified region markers linked to downy mildew resistance in broccoli. J Am Soc Hortic Sci 127:597–601

    CAS  Google Scholar 

  • Huang L, Brooks SA, Li W, Fellers JP, Trick HN, Gill BS (2003) Map-Based cloning of leaf rust resistance gene Lr21 from the large and polyploid genome of bread wheat. Genetics 164:655–664

    PubMed  CAS  Google Scholar 

  • Jensen BD, Hockenhull J, Munk L (1999) Seedling and adult plant resistance to downy mildew (Peronospora parasitica) in cauliflower (Brassica oleracea convar. botrytis var. botrytis). Plant Pathol 48:604–612

    Article  Google Scholar 

  • Konieczny A, Ausubel FM (1993) A procedure for mapping Arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J 4:403–410

    Article  PubMed  CAS  Google Scholar 

  • Lan TH, Delmonte TA, Reischmann KP, Hyman J, Kowalski S, McFerson J, Kresovich S, Paterson AH (2000) EST-enriched comparative map of Brassica oleracea and Arabidopsis thaliana. Genome Res 10:776–788

    Article  PubMed  CAS  Google Scholar 

  • Lukens L, Zou F, Lydiate D, Parkin I, Osborn T (2003) Comparison of a Brassica oleracea genetic map with the genome of Arabidopsis thaliana. Genetics 164:359–372

    PubMed  CAS  Google Scholar 

  • Mahajan V, Gill HS, More TA (1995) Inheritance of downy mildew resistance in Indian cauliflower (group III). Euphytica 86:1–3

    Article  Google Scholar 

  • Martin GB, Bogdanove AJ, Sessa G (2003) Understanding the functions of plant disease resistance proteins. Annu Rev Plant Biol 54:23–61

    Article  PubMed  CAS  Google Scholar 

  • Mayerhofer R, Wilde K, Mayerhofer M, Lydiate D, Bansal VK, Good AG, Parkin I (2005) Complexities of chromosome landing in a highly duplicated genome: Toward Map-based cloning of a gene controlling blackleg resistance in Brassica napus. Genetics 171:1977–1988

    Article  PubMed  CAS  Google Scholar 

  • Natti JJ, Dickson MH, Atkin JD (1967) Resistance of Brassica oleracea varieties to downy mildew. Phytopathology 57:144–147

    Google Scholar 

  • Paran I, Michelmore RW (1993) Development of reliable PCR based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85:985–993

    Article  CAS  Google Scholar 

  • Parker JE, Coleman MJ, Szabò V, Frost LN, Schmidt R, van der Biezen EA, Moores T, Dean C, Daniels M, Jones JDG (1997) The Arabidopsis downy mildew gene RPP5 shares similarity to the Toll and Interleukin-1 receptors with N and L6. Plant Cell 9:879–894

    Article  PubMed  CAS  Google Scholar 

  • Parkin IA, Gulden SM, Sharpe AG, Lukens L, Trick M, Osborn TC, Lydiate DJ (2005) Segmental structure of the Brassica napus genome based on comparative analysis with Arabidopsis thaliana. Genetics 171: 765–781

    Article  PubMed  CAS  Google Scholar 

  • Staal J, Kaliff M, Bohman S, Dixelius C (2006) Transgressive segregation reveals two Arabidopsis TIR-NB-LRR resistance genes effective against Leptosphaeria maculans, causal agent of blackleg disease. Plant J 46:218–230

    Article  PubMed  CAS  Google Scholar 

  • Van Ooijen JW, Voorrips RE (2001) JoinMap 3.0, Software for the calculation of genetic linkage maps. Plant Research International, Wageningen, The Netherlands

    Google Scholar 

Download references

Acknowledgements

This research was supported by the Ministério para a Ciência e a Tecnologia - Fundação para a Ciência e a Tecnologia (MCES-FCT) POCTI/2000 – AGR/33309/99 “Genetic and molecular characterisation of resistance to downy mildew in Brassica oleracea”. Mário Farinhó was the recipient of the Ph.D. fellowship GGPXXI/BD/3834/96 awarded by MCES-FCT. José Leitão was the recipient of the sabbatical fellowship Proj. 250/03 awarded by Fundação Luso-Americana para o Desenvolvimento.

Author information

Authors and Affiliations

  1. FERN, CDCTPV, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal

    Mário Farinhó & José Leitão

  2. Instituto Nacional de Investigação Agrária, Estação Agronómica Nacional, 2780-505, Oeiras, Portugal

    Paula Coelho

  3. Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Tapada da Ajuda, 1349-017, Lisboa, Portugal

    António Monteiro

Authors
  1. Mário Farinhó
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Paula Coelho
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. António Monteiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. José Leitão
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to José Leitão.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Farinhó, M., Coelho, P., Monteiro, A. et al. SCAR and CAPS markers flanking the Brassica oleracea L. Pp523 downy mildew resistance locus demarcate a genomic region syntenic to the top arm end of Arabidopsis thaliana L. chromosome 1. Euphytica 157, 215–221 (2007). https://doi.org/10.1007/s10681-007-9414-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10681-007-9414-6

Keywords

Search

Navigation